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Questions and Answers
What is the purpose of the holder method mentioned in the text?
What is the purpose of the holder method mentioned in the text?
- To ensure sterility by boiling for 10 minutes
- To kill Coxiella burnetti at 63-66°C for 30 minutes (correct)
- To kill vegetative organisms at 100°C for 10 minutes
- To destroy germinated spores on each of 3 successive days
What is the main purpose of Tyndallization as described in the text?
What is the main purpose of Tyndallization as described in the text?
- Killing germinated spores over a period of 3 successive days (correct)
- Ensuring sterility by steaming for 90 minutes at 100°C
- Precautionary measure for filtering heat-labile materials
- Destroying vegetative organisms and spores in a single day
Which physical method is primarily used for sterilization of heat-labile materials?
Which physical method is primarily used for sterilization of heat-labile materials?
- Tyndallization
- Boiling at 100°C
- The flash method
- Filtration through mechanical sieving (correct)
What temperature and duration are typically used in the flash method for sterilization?
What temperature and duration are typically used in the flash method for sterilization?
In the context of vaccine preparation, what temperature is used and for how long?
In the context of vaccine preparation, what temperature is used and for how long?
What is the main means of dry heat sterilization?
What is the main means of dry heat sterilization?
Which term refers to the removal of all viable micro-organisms, including bacterial and fungal spores?
Which term refers to the removal of all viable micro-organisms, including bacterial and fungal spores?
What is the term for killing bacteria?
What is the term for killing bacteria?
Which process involves total destruction by burning?
Which process involves total destruction by burning?
What does a bacteriostatic substance do?
What does a bacteriostatic substance do?
Which substance is used on living tissues for inhibiting or destroying microorganisms?
Which substance is used on living tissues for inhibiting or destroying microorganisms?
What is the purpose of using cotton wool plug in tubes during air filtration?
What is the purpose of using cotton wool plug in tubes during air filtration?
Which type of radiation is used to sterilize plastics, pharmaceutical products, and preserve foods?
Which type of radiation is used to sterilize plastics, pharmaceutical products, and preserve foods?
What is the purpose of using Seitz filter (compressed asbestos) and sintered glass filter (Berkefield) during filtration?
What is the purpose of using Seitz filter (compressed asbestos) and sintered glass filter (Berkefield) during filtration?
What is the effect of ultraviolet light on nucleic acids?
What is the effect of ultraviolet light on nucleic acids?
Which type of radiation absorbs water molecules and releases microwave energy as heat?
Which type of radiation absorbs water molecules and releases microwave energy as heat?
How does gamma radiation and X-rays affect proteins and nucleic acids?
How does gamma radiation and X-rays affect proteins and nucleic acids?
What is the primary method used in the sterilization of surgical instruments employing Infra-Red radiation?
What is the primary method used in the sterilization of surgical instruments employing Infra-Red radiation?
What is the temperature range typically used in Hot Air Sterilizers?
What is the temperature range typically used in Hot Air Sterilizers?
In Autoclaving, what is the temperature required for sterilization at 15 lbs pressure for 15 minutes?
In Autoclaving, what is the temperature required for sterilization at 15 lbs pressure for 15 minutes?
Which method ensures the destruction of non-spore forming organisms like Mycobacteria?
Which method ensures the destruction of non-spore forming organisms like Mycobacteria?
What method can be employed to monitor autoclave sterilization through a dark color change indicator?
What method can be employed to monitor autoclave sterilization through a dark color change indicator?
What type of autoclave is designed for placement on a table-top?
What type of autoclave is designed for placement on a table-top?
Which agent denatures proteins by altering the pH of the organism's environment?
Which agent denatures proteins by altering the pH of the organism's environment?
What is the main purpose of using ethanol in sterilizing skin and disinfecting clinical thermometers?
What is the main purpose of using ethanol in sterilizing skin and disinfecting clinical thermometers?
Which agent is bactericidal specifically for Neisseria gonorrhoeae?
Which agent is bactericidal specifically for Neisseria gonorrhoeae?
Why are alcohols like ethanol considered ineffective against viruses?
Why are alcohols like ethanol considered ineffective against viruses?
Which agent is commonly used as an antiseptic for cuts and burns?
Which agent is commonly used as an antiseptic for cuts and burns?
How do heavy metals like silver nitrate affect proteins and other organic molecules?
How do heavy metals like silver nitrate affect proteins and other organic molecules?
What is the main purpose of using cotton wool plugs in tubes during air filtration?
What is the main purpose of using cotton wool plugs in tubes during air filtration?
How does microwave radiation contribute to sterilization?
How does microwave radiation contribute to sterilization?
What is the primary function of a Seitz filter (compressed asbestos) and sintered glass filter (Berkefield) during filtration?
What is the primary function of a Seitz filter (compressed asbestos) and sintered glass filter (Berkefield) during filtration?
What is the effect of ultraviolet light on microorganisms?
What is the effect of ultraviolet light on microorganisms?
How do Gamma radiation and X-rays primarily contribute to sterilization?
How do Gamma radiation and X-rays primarily contribute to sterilization?
What is the primary function of a syringe filter in the filtration process?
What is the primary function of a syringe filter in the filtration process?
What is the main difference between Infra-Red radiation and Hot Air Sterilization?
What is the main difference between Infra-Red radiation and Hot Air Sterilization?
Why is autoclaving considered a widely used method for sterilization?
Why is autoclaving considered a widely used method for sterilization?
What is the purpose of autoclave control mechanisms like temperature record and indicators?
What is the purpose of autoclave control mechanisms like temperature record and indicators?
What is the key difference between a satisfactory run and an unsatisfactory run in autoclaving?
What is the key difference between a satisfactory run and an unsatisfactory run in autoclaving?
Why is pasteurization typically carried out?
Why is pasteurization typically carried out?
Which method ensures the destruction of non-spore forming organisms like Mycobacteria?
Which method ensures the destruction of non-spore forming organisms like Mycobacteria?
What is the main difference between sterilization and disinfection?
What is the main difference between sterilization and disinfection?
What is the primary purpose of asepsis?
What is the primary purpose of asepsis?
Which method of dry heat sterilization involves total destruction by burning?
Which method of dry heat sterilization involves total destruction by burning?
What is the temperature range typically used in Hot Air Sterilizers?
What is the temperature range typically used in Hot Air Sterilizers?
Which substance is specifically used on living tissues for inhibiting or destroying microorganisms?
Which substance is specifically used on living tissues for inhibiting or destroying microorganisms?
Which of the following agents is NOT effective against viruses?
Which of the following agents is NOT effective against viruses?
What heavy metal is widely used as an antiseptic for its ability to precipitate proteins and organic molecules?
What heavy metal is widely used as an antiseptic for its ability to precipitate proteins and organic molecules?
Which agent is specifically effective against Neisseria gonorrhoeae?
Which agent is specifically effective against Neisseria gonorrhoeae?
Which oxidizing agent is commonly used as a sporicidal and effective disinfectant?
Which oxidizing agent is commonly used as a sporicidal and effective disinfectant?
Which alkali is known to kill vegetative forms?
Which alkali is known to kill vegetative forms?
'Water is essential for activity' is a characteristic associated with which type of agent?
'Water is essential for activity' is a characteristic associated with which type of agent?
Which disinfectant is 10 times more effective than formaldehyde and is used as a 2% concentration?
Which disinfectant is 10 times more effective than formaldehyde and is used as a 2% concentration?
Why are alkylating agents like glutaraldehyde and formaldehyde preferred for disinfection in invasive procedures?
Why are alkylating agents like glutaraldehyde and formaldehyde preferred for disinfection in invasive procedures?
What makes high-level disinfection methods like activated glutaraldehyde, hydrogen peroxide, and formaldehyde the method of choice for critical items?
What makes high-level disinfection methods like activated glutaraldehyde, hydrogen peroxide, and formaldehyde the method of choice for critical items?
What characteristic makes glutaraldehyde different from formaldehyde in terms of disinfection effectiveness?
What characteristic makes glutaraldehyde different from formaldehyde in terms of disinfection effectiveness?
Why is formaldehyde commonly used for preserving tissues and as an embalming fluid?
Why is formaldehyde commonly used for preserving tissues and as an embalming fluid?
What makes glutaraldehyde suitable for disinfecting medical instruments like endoscopes that cannot be sterilized by steam?
What makes glutaraldehyde suitable for disinfecting medical instruments like endoscopes that cannot be sterilized by steam?
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