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Questions and Answers
What is the purpose of cleaning in the context of decontamination?
What is the purpose of cleaning in the context of decontamination?
- To destroy all living forms of microbes including spores.
- To remove foreign material like dirt and organic matter before disinfection and sterilization. (correct)
- To apply chemical compounds topically on animate surfaces like skin.
- To reduce pathogenic microbes to a safe level for handling and disposal.
What is the primary difference between disinfection and sterilization?
What is the primary difference between disinfection and sterilization?
- Disinfection destroys most but not necessarily all pathogenic microbes, while sterilization kills all living forms of microbes including spores. (correct)
- Disinfection is a process that removes foreign material, while sterilization involves applying antiseptics topically on animate surfaces.
- Disinfection is done with soap and water, while sterilization is achieved by using detergents.
- Disinfection involves the use of chemical compounds, while sterilization involves physical methods like heat.
What are antiseptics primarily used for?
What are antiseptics primarily used for?
- To be applied topically on animate surfaces like skin. (correct)
- To achieve the destruction of most pathogenic microbes or their spores.
- To remove foreign material before disinfection and sterilization.
- To reduce pathogenic microbes to a safe level for handling and disposal.
Why is safety in the laboratory mentioned as an importance of sterilization and disinfection?
Why is safety in the laboratory mentioned as an importance of sterilization and disinfection?
Which physical method is mentioned for achieving sterilization?
Which physical method is mentioned for achieving sterilization?
What is the key difference between antiseptics and disinfectants?
What is the key difference between antiseptics and disinfectants?
Which method of sterilization is used for heat sensitive fluids like serum, CHO, and urea?
Which method of sterilization is used for heat sensitive fluids like serum, CHO, and urea?
At what temperature is milk heated during the process of pasteurization for disinfection?
At what temperature is milk heated during the process of pasteurization for disinfection?
What is the purpose of using a hot air oven in sterilization?
What is the purpose of using a hot air oven in sterilization?
Which method of sterilization involves exposure to irradiation that kills microbes by coagulation and denaturation of proteins and enzymes?
Which method of sterilization involves exposure to irradiation that kills microbes by coagulation and denaturation of proteins and enzymes?
What is the purpose of tyndallization in sterilization?
What is the purpose of tyndallization in sterilization?
What is the temperature requirement for steaming as a method of sterilization?
What is the temperature requirement for steaming as a method of sterilization?
In which method of sterilization are objects exposed to heat to kill microbes by coagulation of proteins, denaturation of enzymes, and oxidation?
In which method of sterilization are objects exposed to heat to kill microbes by coagulation of proteins, denaturation of enzymes, and oxidation?
What is the purpose of boiling as a method of disinfection?
What is the purpose of boiling as a method of disinfection?
Study Notes
Decontamination and Sterilization
- Cleaning is an essential step in decontamination to remove organic matter and reduce microbial load.
Disinfection and Sterilization
- Disinfection reduces the number of microorganisms, but does not eliminate all microorganisms, whereas sterilization eliminates all microorganisms.
Antiseptics
- Antiseptics are primarily used to disinfect skin and mucous membranes, reducing the number of microorganisms on living tissues.
Laboratory Safety
- Safety in the laboratory is crucial, as sterilization and disinfection help prevent the transmission of infectious diseases and protect laboratory personnel.
Sterilization Methods
- Heat is a physical method used for achieving sterilization.
- Filtration is used for heat-sensitive fluids like serum, CHO, and urea.
- Hot air oven is used for sterilization, particularly for dry heat sterilization.
- Ionizing radiation is used for sterilization, involving exposure to irradiation that kills microbes by coagulation and denaturation of proteins and enzymes.
- Tyndallization is used for sterilization, involving intermittent sterilization to kill highly resistant spores.
- Autoclaving, a type of steaming, is used for sterilization, requiring a temperature of 121°C.
Pasteurization
- Milk is heated to 63°C for 30 minutes during pasteurization for disinfection.
Boiling
- Boiling is a method of disinfection, killing microorganisms by coagulation of proteins, denaturation of enzymes, and oxidation.
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Description
This quiz covers the concepts of decontamination, cleaning, disinfection, and sterilization in the context of reducing pathogenic microbes to a safe level for handling and disposal. It includes key information about the procedures involved in each process.
