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Stem Cells in Transplant and Regenerative Medicine

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Stem Cells in Transplant and Regenerative Medicine

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Questions and Answers

What is the primary role of Nanog in embryonic stem cells (ESCs)?

Maintaining pluripotency in concert with other factors (correct)

Promoting cell division and proliferation

Controlling the expression levels of Oct4 and Sox2

Regulating endoderm formation during differentiation

What happens when Nanog is knocked down or depleted in ESCs?

ESCs remain in an undifferentiated state

ESCs differentiate into parietal endoderm-like cells (correct)

ESCs differentiate into various cell types

ESCs undergo apoptosis or cell death

What is the significance of the precise expression level of Oct4 in ESCs?

It regulates the expression of Nanog and Sox2

It is required for self-renewal and pluripotency maintenance (correct)

It controls the forward and side scatter properties of ESCs

It determines the fate of ESC differentiation

What is the primary principle behind flow cytometry?

Measuring the size and complexity of cells using light scatter Signup and view all the answers

What is the purpose of transfecting cells with fluorescent proteins in flow cytometry?

To quantify the expression levels of specific proteins Signup and view all the answers

Which of the following statements about ESCs is true?

ESCs can maintain pluripotency indefinitely in culture Signup and view all the answers

What is the primary advantage of flow cytometry over other cell analysis techniques?

It can analyze a large number of cells rapidly Signup and view all the answers

Which of the following statements about the transcription factors Oct4, Sox2, and Nanog is correct?

Their knockdown causes ESCs to lose pluripotency and differentiate Signup and view all the answers

What does the forward scatter (FSC) parameter in flow cytometry measure?

The relative size of the cell Signup and view all the answers

What is the purpose of using a buffered salt-based solution in flow cytometry?

To maintain the viability of cells during analysis Signup and view all the answers