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Questions and Answers
What characteristics distinguish rare strains of aureus that require specific growth conditions?
What characteristics distinguish rare strains of aureus that require specific growth conditions?
They are fastidious, requiring CO2, hemin, or menadione for growth and form small colonies with 1/10 the size of wild type strains after 48 hours.
Describe the microscopic appearance of aureus in terms of cell arrangement.
Describe the microscopic appearance of aureus in terms of cell arrangement.
Aureus appears as spherical cells that can be found singly, in pairs, or in clusters.
What are the cultural characteristics of aureus colonies on BAP agar plates?
What are the cultural characteristics of aureus colonies on BAP agar plates?
Aureus colonies appear creamy, white, or light gold, typically measuring 4mm-8mm, with a buttery texture.
How does aureus react to lysostaphin compared to other staphylococci species?
How does aureus react to lysostaphin compared to other staphylococci species?
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Explain the significance of aureus being B-hemolytic and its implications on antibiotic resistance.
Explain the significance of aureus being B-hemolytic and its implications on antibiotic resistance.
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What are the newly suggested strains of Staphylococci mentioned in the content?
What are the newly suggested strains of Staphylococci mentioned in the content?
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Describe the morphological characteristics of Staphylococcus aureus.
Describe the morphological characteristics of Staphylococcus aureus.
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What role does the peptidoglycan layer play in Staphylococcus cell structure?
What role does the peptidoglycan layer play in Staphylococcus cell structure?
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What type of hemolysis is observed with Staphylococcus schleiferi on sheep blood agar?
What type of hemolysis is observed with Staphylococcus schleiferi on sheep blood agar?
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Which selective media can be used to identify lactose fermentation in Staphylococcus species?
Which selective media can be used to identify lactose fermentation in Staphylococcus species?
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What metabolic conditions are preferred for the growth of some Staphylococcus strains?
What metabolic conditions are preferred for the growth of some Staphylococcus strains?
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What is the major protein component of the Staphylococcus cell wall?
What is the major protein component of the Staphylococcus cell wall?
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What type of colonies appear on Mac Conkey agar when lactose is fermented?
What type of colonies appear on Mac Conkey agar when lactose is fermented?
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What is the optimum temperature range for extensive exfoliative dermatitis?
What is the optimum temperature range for extensive exfoliative dermatitis?
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Which demographic is primarily affected by extensive exfoliative dermatitis?
Which demographic is primarily affected by extensive exfoliative dermatitis?
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Describe the appearance of colonies observed on nutrient agar for the relevant condition.
Describe the appearance of colonies observed on nutrient agar for the relevant condition.
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What is the significance of the pH range 7.4-7.6 in relation to exfoliative dermatitis?
What is the significance of the pH range 7.4-7.6 in relation to exfoliative dermatitis?
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What is toxic shock syndrome (TSS) associated with, particularly among women?
What is toxic shock syndrome (TSS) associated with, particularly among women?
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What is the role of the epidermolytic toxin in the condition discussed?
What is the role of the epidermolytic toxin in the condition discussed?
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What characteristic appearance is noted on nutrient slope media?
What characteristic appearance is noted on nutrient slope media?
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What pigment is produced by the bacteria in this context, and what is its characteristic?
What pigment is produced by the bacteria in this context, and what is its characteristic?
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What is the purpose of the slide method in detecting coagulase positive colonies?
What is the purpose of the slide method in detecting coagulase positive colonies?
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Describe the role of hemolysin as a cytotoxin.
Describe the role of hemolysin as a cytotoxin.
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What are the types of hemolysins and their primary actions?
What are the types of hemolysins and their primary actions?
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How does beta-hemolysin exhibit enhanced hemolytic activity?
How does beta-hemolysin exhibit enhanced hemolytic activity?
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What is the procedure for the tube coagulase test, and what does a positive result indicate?
What is the procedure for the tube coagulase test, and what does a positive result indicate?
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What should be done if no clot appears in the tube coagulase test after 4 hours?
What should be done if no clot appears in the tube coagulase test after 4 hours?
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What is the significance of coagulase production in necrotizing skin infections?
What is the significance of coagulase production in necrotizing skin infections?
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Which organisms are noted as other slide coagulase positive species?
Which organisms are noted as other slide coagulase positive species?
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What is the significance of mannitol salt agar in identifying MRSA?
What is the significance of mannitol salt agar in identifying MRSA?
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What genetic element is responsible for MRSA's resistance to penicillinase-resistant penicillin?
What genetic element is responsible for MRSA's resistance to penicillinase-resistant penicillin?
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What is a significant risk factor for acquiring infections from Staphylococcus epidermidis?
What is a significant risk factor for acquiring infections from Staphylococcus epidermidis?
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What gene is associated with methicillin resistance in Staphylococcus epidermidis?
What gene is associated with methicillin resistance in Staphylococcus epidermidis?
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How does a positive Chromogenic test indicate the presence of MRSA?
How does a positive Chromogenic test indicate the presence of MRSA?
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What morphological feature distinguishes MRSA from species like S.saprophyticus on MSA?
What morphological feature distinguishes MRSA from species like S.saprophyticus on MSA?
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Name two common infections caused by Staphylococcus epidermidis.
Name two common infections caused by Staphylococcus epidermidis.
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What is the primary mechanism through which Staphylococcus epidermidis adheres to medical devices?
What is the primary mechanism through which Staphylococcus epidermidis adheres to medical devices?
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What role does the Durham tube play in the identification of staphylococci?
What role does the Durham tube play in the identification of staphylococci?
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How does Staphylococcus saprophyticus differ from other coagulase-negative staphylococci regarding novobiocin resistance?
How does Staphylococcus saprophyticus differ from other coagulase-negative staphylococci regarding novobiocin resistance?
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Why do isolates of oxacillin-resistant S.aureus get classified as MRSA?
Why do isolates of oxacillin-resistant S.aureus get classified as MRSA?
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How does the high percentage of resistance among coagulase-negative staphylococci affect treatment options?
How does the high percentage of resistance among coagulase-negative staphylococci affect treatment options?
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What type of bacteria are coagulase-negative staphylococci (CoNS), and how are they typically identified?
What type of bacteria are coagulase-negative staphylococci (CoNS), and how are they typically identified?
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What is indicated by the jet black colonies formed on tellurite glycine agar?
What is indicated by the jet black colonies formed on tellurite glycine agar?
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In what clinical scenarios might Staphylococcus epidermidis be particularly virulent?
In what clinical scenarios might Staphylococcus epidermidis be particularly virulent?
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What type of colonies does Staphylococcus epidermidis produce on Blood Agar Plates (BAP)?
What type of colonies does Staphylococcus epidermidis produce on Blood Agar Plates (BAP)?
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Study Notes
Staphylococcus
- Derived from Greek words: "staphle" (bunches of grapes) and "kokkos" (berry)
- Gram-positive cocci in grape-like clusters
- Belong to the family Micrococcaceae
- Normal inhabitants of skin, mucous membranes, and intestines
- Common cause of suppurative infections
- Catalase-producing, facultatively anaerobic (except S. saccharolyticus)
Differences between Staphylococcus and Micrococcus
- Morphology: Staphylococci form clusters; Micrococci form tetrads or pairs
- Clinical Significance: Staphylococci are more important clinically than Micrococci
- Lysostaphin Sensitivity: Staphylococci are lysed by lysostaphin; Micrococci are resistant
- Furazolidone Sensitivity: Staphylococci are susceptible; Micrococci are resistant
- Bacitracin Sensitivity: Staphylococci are resistant to higher concentrations; Micrococci susceptible
- Microdase Test: Staphylococci are negative; Micrococci are positive
- Oxygen Requirements: Staphylococci are facultative anaerobes; Micrococci are obligate aerobes
- Carbohydrate Utilization: Staphylococci fermentatively; Micrococci oxidatively or not at all
Current Classification of Staphylococci
- ~32 species
- Coagulase-positive: S. aureus, S. intermedius, S. pseudintermedius, S. hyicus, S. delphini, S. lutrae, S. agnetis
- Coagulase-negative: S. epidermidis, S.hemolyticus, S. saprophyticus, S. hominis, S. capitis
Staphylococcus aureus
- Morphology: Gram-positive cocci in clusters, often pairs or short chains
- Virulence Factors: Enterotoxins, toxic shock syndrome toxin 1 (TSST-1), exfoliative toxins, cytolytic toxins, enzymes, and Protein A
- Clinical Significance: Important pathogen responsible for many types of infections
- Culture Characteristics: Grows at 10-42°C (optimum 37°C), pH 7.4-7.6. Creates large, circular, smooth, shiny, opaque, and easily emulsifiable colonies. Yellow-pigmented colonies which do not diffuse into the medium, the pigment is carotene-like lipoprotein. Optimally, this pigment production occurs at 22C or when specific substances are added.
- Biochemical Reactions: Catalase +, Indole -, VP +, MR +, Urease +, Nitrate reduction +, Phosphatase +, Mannitol fermentation +
Additional Staphylococcus aureus Information
- Most virulent species. Coagulase (+)
- Colonies appear golden yellow
- B-hemolytic on blood agar
- Can be cultivated in media with 7.5-10% NaCl
- Predominantly responsible for skin, wound, and deep tissue infections
Staphylococcus aureus: Related Infections and Diseases
- Food Poisoning: Associated with enterotoxins (A, B, and D)
- Scalded Skin Syndrome (SSS): Epidermolytic toxins A and B cause exfoliation of the epidermis
- Toxic Shock Syndrome (TSS): TSST-1 induces an extensive, potentially fatal, systemic reaction.
- Deep Infections: Osteomyelitis, septic arthritis
- Cutaneous infections: Folliculitis, furuncles (boils), carbuncles, impetigo, abscess
Additional Staphylococcal Information (from other pages)
- Deoxyribonuclease (DNase) & Phosphatase: Lowers exudate viscosity; destroys DNA
- B-Lactamase: Breaks down penicillin; common in clinical isolates
- Enterotoxin (Heat-stable): Vomiting stimulator caused by vagus nerve stimulation
- Leukocidin / Panton-Valentine Leukocidin (PVL): Lethal to neutrophils; pore-forming exotoxin
- Hemolysin (Cytotoxin): Causes anemia; involved in iron acquisition, including types alpha, beta, and gamma
- Exfoliatin Serotypes A & B: Disrupt epidermal cell junctions
- Tests for Identification: Coagulase, mannitol fermentation, catalase, novobiocin susceptibility, and more.
- Methicillin-Resistant Staphylococcus aureus (MRSA) : Resistant to common antibiotics, commonly hospital-acquired
- Coagulase-negative Staphylococcus (CONS): Do not produce exotoxins causing TSS
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Description
Test your knowledge on Staphylococcus and Micrococcus! This quiz covers their morphological differences, clinical significance, and sensitivity to various agents. Explore the vital role these bacteria play in human health and disease.