Spore-Forming Bacteria Quiz

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Questions and Answers

Which bacterium is able to undergo spore formation according to the text?

Clostridium sporogenes ATCC 11437 (correct)
Bacillus cereus ATCC 10876
Geobacillus stearothermophilus ATCC 12980
Bacillus subtilis ATCC 6051

What should be added to the agar medium to stimulate sporulation?

Zinc sulfate
Manganese sulphate (correct)
Calcium chloride
Potassium nitrate

How long should the plates be incubated depending on the growth pace of an organism?

5-7 days
2-5 days (correct)
7-10 days
1-2 days

What should be used to dislodge the growth from the plates?

Sterile L-spreader (D) Signup and view all the answers

How much of 0.5% Tween 20 in MRD should be used to flood the plates upon seeing visible growth?

10ml (C) Signup and view all the answers

How many spread plates of a neat liquid culture should be prepared using microbeads from Hygiena culture collection?

At least three (B) Signup and view all the answers

What should be prepared and autoclaved before flooding the plates with 0.5% Tween 20 in MRD?

Maximum Recovery Diluent (A) Signup and view all the answers

How should the obtained cell suspension from each plate be transferred?

Using a sterile pipette (C) Signup and view all the answers

Which of the following is the correct temperature for heat shocking the organism?

95°C (A) Signup and view all the answers

How many times should the process of transferring the supernatant and resuspending in 0.5% Tween-20 MRD be repeated?

3 (D) Signup and view all the answers

What is the starting dilution of the yeast cells in sporulation media?

1x10^7 cells/ml (C) Signup and view all the answers

How many days should the yeast cells be allowed to sporulate?

5-7 days (D) Signup and view all the answers

What is the concentration of Tween 20 in the autoclaved MRD?

0.05% (B) Signup and view all the answers

How many plates should be flooded with MRD with 0.05% Tween 20?

3 (A) Signup and view all the answers

What should be used to dislodge the spores from the plate to the solution?

L-shaped spreader (D) Signup and view all the answers

How many universals should be labelled with the microorganism name and number?

3 (A) Signup and view all the answers

Which of the following is the correct procedure for counting spores using a hemocytometer?

Transfer 5ml of neat, -1 and –2 dilution onto a hemocytometer and count the spores using a microscope (A) Signup and view all the answers

What is the recommended dilution for preparing the spore suspensions for counting spores using a hemocytometer?

Neat to –6 dilution (D) Signup and view all the answers

How long should the spores settle in the hemocytometer chamber before counting?

2 minutes (B) Signup and view all the answers

How many cells should be present in the central square of the hemocytometer for accurate counting?

At least 100 cells (C) Signup and view all the answers

What should be done to avoid contamination and counting error when using a hemocytometer?

Clean the hemocytometer and cover glass with 70% ethanol (B) Signup and view all the answers

What is the purpose of centrifuging the universals with spores at room temperature for 10 minutes at 4000rpm?

To balance the centrifuge (D) Signup and view all the answers

How many times should the procedure of adding fresh MRD with 0.05% Tween 20 and repeating be repeated?

Three times (A) Signup and view all the answers

What is the alternative and faster way of assessing the spore counts in 1ml of spore suspension?

Transfer 5ml of neat, -1 and –2 dilution onto a hemocytometer and count the spores using a microscope (B) Signup and view all the answers

What is the formula for calculating the cell density using the spore counts obtained?

$\text{Cell Density} = \text{Number of Spores} \times \text{Dilution Factor}$ (A) Signup and view all the answers

Flashcards

Spore-forming bacterium

Clostridium sporogenes ATCC 11437 is capable of forming spores.

Stimulating sporulation

Manganese sulphate should be added to the agar medium.