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Questions and Answers
Which bacterium is able to undergo spore formation according to the text?
Which bacterium is able to undergo spore formation according to the text?
- Clostridium sporogenes ATCC 11437 (correct)
- Bacillus cereus ATCC 10876
- Geobacillus stearothermophilus ATCC 12980
- Bacillus subtilis ATCC 6051
What should be added to the agar medium to stimulate sporulation?
What should be added to the agar medium to stimulate sporulation?
- Zinc sulfate
- Manganese sulphate (correct)
- Calcium chloride
- Potassium nitrate
How long should the plates be incubated depending on the growth pace of an organism?
How long should the plates be incubated depending on the growth pace of an organism?
- 5-7 days
- 2-5 days (correct)
- 7-10 days
- 1-2 days
What should be used to dislodge the growth from the plates?
What should be used to dislodge the growth from the plates?
How much of 0.5% Tween 20 in MRD should be used to flood the plates upon seeing visible growth?
How much of 0.5% Tween 20 in MRD should be used to flood the plates upon seeing visible growth?
How many spread plates of a neat liquid culture should be prepared using microbeads from Hygiena culture collection?
How many spread plates of a neat liquid culture should be prepared using microbeads from Hygiena culture collection?
What should be prepared and autoclaved before flooding the plates with 0.5% Tween 20 in MRD?
What should be prepared and autoclaved before flooding the plates with 0.5% Tween 20 in MRD?
How should the obtained cell suspension from each plate be transferred?
How should the obtained cell suspension from each plate be transferred?
Which of the following is the correct temperature for heat shocking the organism?
Which of the following is the correct temperature for heat shocking the organism?
How many times should the process of transferring the supernatant and resuspending in 0.5% Tween-20 MRD be repeated?
How many times should the process of transferring the supernatant and resuspending in 0.5% Tween-20 MRD be repeated?
What is the starting dilution of the yeast cells in sporulation media?
What is the starting dilution of the yeast cells in sporulation media?
How many days should the yeast cells be allowed to sporulate?
How many days should the yeast cells be allowed to sporulate?
What is the concentration of Tween 20 in the autoclaved MRD?
What is the concentration of Tween 20 in the autoclaved MRD?
How many plates should be flooded with MRD with 0.05% Tween 20?
How many plates should be flooded with MRD with 0.05% Tween 20?
What should be used to dislodge the spores from the plate to the solution?
What should be used to dislodge the spores from the plate to the solution?
How many universals should be labelled with the microorganism name and number?
How many universals should be labelled with the microorganism name and number?
Which of the following is the correct procedure for counting spores using a hemocytometer?
Which of the following is the correct procedure for counting spores using a hemocytometer?
What is the recommended dilution for preparing the spore suspensions for counting spores using a hemocytometer?
What is the recommended dilution for preparing the spore suspensions for counting spores using a hemocytometer?
How long should the spores settle in the hemocytometer chamber before counting?
How long should the spores settle in the hemocytometer chamber before counting?
How many cells should be present in the central square of the hemocytometer for accurate counting?
How many cells should be present in the central square of the hemocytometer for accurate counting?
What should be done to avoid contamination and counting error when using a hemocytometer?
What should be done to avoid contamination and counting error when using a hemocytometer?
What is the purpose of centrifuging the universals with spores at room temperature for 10 minutes at 4000rpm?
What is the purpose of centrifuging the universals with spores at room temperature for 10 minutes at 4000rpm?
How many times should the procedure of adding fresh MRD with 0.05% Tween 20 and repeating be repeated?
How many times should the procedure of adding fresh MRD with 0.05% Tween 20 and repeating be repeated?
What is the alternative and faster way of assessing the spore counts in 1ml of spore suspension?
What is the alternative and faster way of assessing the spore counts in 1ml of spore suspension?
What is the formula for calculating the cell density using the spore counts obtained?
What is the formula for calculating the cell density using the spore counts obtained?
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