Spore-Forming Bacteria Quiz
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Questions and Answers

Which bacterium is able to undergo spore formation according to the text?

  • Clostridium sporogenes ATCC 11437 (correct)
  • Bacillus cereus ATCC 10876
  • Geobacillus stearothermophilus ATCC 12980
  • Bacillus subtilis ATCC 6051

    • What should be added to the agar medium to stimulate sporulation?

  • Zinc sulfate
  • Manganese sulphate (correct)
  • Calcium chloride
  • Potassium nitrate

    • How long should the plates be incubated depending on the growth pace of an organism?

  • 5-7 days
  • 2-5 days (correct)
  • 7-10 days
  • 1-2 days

    • What should be used to dislodge the growth from the plates?

    <p>Sterile L-spreader</p> Signup and view all the answers

    How much of 0.5% Tween 20 in MRD should be used to flood the plates upon seeing visible growth?

    <p>10ml</p> Signup and view all the answers

    How many spread plates of a neat liquid culture should be prepared using microbeads from Hygiena culture collection?

    <p>At least three</p> Signup and view all the answers

    What should be prepared and autoclaved before flooding the plates with 0.5% Tween 20 in MRD?

    <p>Maximum Recovery Diluent</p> Signup and view all the answers

    How should the obtained cell suspension from each plate be transferred?

    <p>Using a sterile pipette</p> Signup and view all the answers

    Which of the following is the correct temperature for heat shocking the organism?

    <p>95°C</p> Signup and view all the answers

    How many times should the process of transferring the supernatant and resuspending in 0.5% Tween-20 MRD be repeated?

    <p>3</p> Signup and view all the answers

    What is the starting dilution of the yeast cells in sporulation media?

    <p>1x10^7 cells/ml</p> Signup and view all the answers

    How many days should the yeast cells be allowed to sporulate?

    <p>5-7 days</p> Signup and view all the answers

    What is the concentration of Tween 20 in the autoclaved MRD?

    <p>0.05%</p> Signup and view all the answers

    How many plates should be flooded with MRD with 0.05% Tween 20?

    <p>3</p> Signup and view all the answers

    What should be used to dislodge the spores from the plate to the solution?

    <p>L-shaped spreader</p> Signup and view all the answers

    How many universals should be labelled with the microorganism name and number?

    <p>3</p> Signup and view all the answers

    Which of the following is the correct procedure for counting spores using a hemocytometer?

    <p>Transfer 5ml of neat, -1 and –2 dilution onto a hemocytometer and count the spores using a microscope</p> Signup and view all the answers

    What is the recommended dilution for preparing the spore suspensions for counting spores using a hemocytometer?

    <p>Neat to –6 dilution</p> Signup and view all the answers

    How long should the spores settle in the hemocytometer chamber before counting?

    <p>2 minutes</p> Signup and view all the answers

    How many cells should be present in the central square of the hemocytometer for accurate counting?

    <p>At least 100 cells</p> Signup and view all the answers

    What should be done to avoid contamination and counting error when using a hemocytometer?

    <p>Clean the hemocytometer and cover glass with 70% ethanol</p> Signup and view all the answers

    What is the purpose of centrifuging the universals with spores at room temperature for 10 minutes at 4000rpm?

    <p>To balance the centrifuge</p> Signup and view all the answers

    How many times should the procedure of adding fresh MRD with 0.05% Tween 20 and repeating be repeated?

    <p>Three times</p> Signup and view all the answers

    What is the alternative and faster way of assessing the spore counts in 1ml of spore suspension?

    <p>Transfer 5ml of neat, -1 and –2 dilution onto a hemocytometer and count the spores using a microscope</p> Signup and view all the answers

    What is the formula for calculating the cell density using the spore counts obtained?

    <p>$\text{Cell Density} = \text{Number of Spores} \times \text{Dilution Factor}$</p> Signup and view all the answers

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