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Questions and Answers
What do all the mentioned techniques measure?
What do all the mentioned techniques measure?
- Concentration of a sample
- Chirality of a sample
- Types of polarization
- Light in different configurations (correct)
At what angle is UV-Vis absorbance transmitted?
At what angle is UV-Vis absorbance transmitted?
- 90º
- 180º (correct)
- 135º
- 45º
What is measured when circularly polarized light is used as input?
What is measured when circularly polarized light is used as input?
- Fluorescence emission
- Concentration of a sample
- UV-Vis absorbance
- Circular Dichroism (CD) (correct)
What property of a sample does Circular Dichroism (CD) measure?
What property of a sample does Circular Dichroism (CD) measure?
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At what angle is fluorescence emission measured?
At what angle is fluorescence emission measured?
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Study Notes
Measuring Light Configurations
- There are different methods to measure light in various configurations:
- UV-Vis absorbance measures light transmitted at 180º.
- Fluorescence emission measures light at 90º.
- Circular Dichroism (CD) measures the chirality or "handedness" of a sample using circularly polarized light as input.
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