Spectrophotometry Exercises
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Questions and Answers

What is the absorbance value for a 55% T reading?

0.255

What is the absorbance value given a transmittance ratio of 0.40?

0.398

What is the %T value given an absorbance value of 0.835?

0.018

Calculate the transmittance if the intensity of the incident light is 0.50 W/m² and the light entering the detector is 0.36 W/m².

<p>0.72</p> Signup and view all the answers

Calculate the absorbance if the intensity of the incident light is 0.50 W/m² and the light entering the detector is 0.36 W/m².

<p>0.144</p> Signup and view all the answers

What is the molar absorptivity of a 0.25M solution with an absorbance of 0.075 at 560nm?

<p>0.30</p> Signup and view all the answers

What will be the absorbance if the concentration is 0.65M and the molar absorptivity is 0.30?

<p>0.195</p> Signup and view all the answers

What is the concentration if the absorbance is 0.450 and molar absorptivity is 0.30?

<p>1.5M</p> Signup and view all the answers

Write a linear equation that fits the absorbance data given in the table.

<p>A = 0.488 * C + 0.036</p> Signup and view all the answers

What is the absorbance when the concentration is 0.482M?

<p>0.234</p> Signup and view all the answers

What is the concentration when the absorbance is 0.521?

<p>approximately 1.0M</p> Signup and view all the answers

Study Notes

Exercise 1

  • A 55% T reading was obtained for the level 1 creatinine control and reagent using the Jaffe method.
  • The question asks for the absorbance value.

Exercise 2

  • Transmittance ratio is 0.40.
  • The question asks for the absorbance value.

Exercise 3

  • Absorbance value is 0.835.
  • The question asks for the %T value.

Exercise 4

  • Intensity of incident light = 0.50w/m2.
  • Intensity of light entering the detector = 0.36w/m2.
  • The question asks to calculate the transmittance and absorbance.

Exercise 5

  • Concentration of solution = 0.25M.
  • Path length = 1cm.
  • Absorbance = 0.075 at 560nm.
  • The question asks for the molar absorptivity of the solution, the absorbance if the concentration is 0.65M, and the concentration if the absorbance is 0.450.

Exercise 6

  • The table shows the relationship between absorbance and concentration of a solution given a path length of 1cm.
  • The question asks to write a linear equation that fits the data, the absorbance when the concentration is 0.482M, and the concentration when the absorbance is 0.521.

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Description

Test your knowledge on spectrophotometry with these exercises. This quiz involves calculating absorbance, transmittance, and molar absorptivity for various conditions. Perfect for students studying chemistry or laboratory techniques.

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