Exercises for Absorbance, Concentration and Transmittance PDF

Exercises for Absorbance, concentration and Transmittance Lecture 3  Exercise 1 A manual creatinine assay was performed using the Jaffe method. A 55% T reading was obtained for the cuvette containing the level 1 creatinine control and reagent. What is the ?absor...

Exercises for Absorbance, concentration and Transmittance Lecture 3  Exercise 1 A manual creatinine assay was performed using the Jaffe method. A 55% T reading was obtained for the cuvette containing the level 1 creatinine control and reagent. What is the ?absorbance value  Exercise 2 Given a transmittance ratio of 0.40, what is the ?absorbance value  Exercise 3 Given an absorbance value of 0.835, what is ?the %T value  Exercise 4 The intensity of the incident light is 0.50w/m2, and the intensity of the light entering the.detector is 0.36w/m2.Calculate the transmittance.1.Calculate the absorbance.2  Exercise 5 A 0.25M solution with a path length of 1cm,.has an absorbance of 0.075 at 560nm What is the molar absorptivity of the.1 ?solution What will be the absorbance if the.2 ?concentration is 0.65M What is the concentration if the absorbance is.3 ?0.450  Exercise 6 The table below shows the relationship between absorbance and concentration of a.solution given a path length of 1cm Concentration (M) Absorbance 0.150 0.072 0.351 0.168 0.617 0.296 0.995 0.458 1.421 0.682  …Cont Write a linear equation that fits the data in a.1.reasonable degree What is the absorbance when the.2 ?concentration is 0.482M What is the concentration when the.3 ?absorbance is 0.521