6 Questions
What is the purpose of a reference cuvette in spectrophotometry?
To calibrate the instrument
In spectrophotometry, what does the Beer-Lambert law describe?
The relationship between light intensity and absorbance
What is the purpose of a monochromator in a spectrophotometer?
To filter out unwanted light
Which type of sample is unsuitable for analysis using a spectrophotometer?
Opaque
What is the relationship between absorbance and concentration in spectrophotometry?
Directly proportional
What is one function that a spectrophotometer cannot perform?
Determine sample temperature
Study Notes
Absorbance
- In spectrophotometry, a reference cuvette is used to calibrate the instrument.
- The Beer-Lambert law describes the relationship between light intensity and absorbance.
- A monochromator in a spectrophotometer filters out unwanted light.
- Opaque samples are unsuitable for analysis using a spectrophotometer.
- Absorbance and concentration have a direct proportional relationship in spectrophotometry.
Test your understanding of spectrophotometry concepts, including the purpose of reference cuvettes and the Beer-Lambert law.
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