Spectrophotometry Fundamentals
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Questions and Answers

What is the purpose of a reference cuvette in spectrophotometry?

  • To dilute the samples
  • To clean the samples
  • To calibrate the instrument (correct)
  • To heat the samples
  • In spectrophotometry, what does the Beer-Lambert law describe?

  • The relationship between pressure and weight
  • The relationship between light intensity and absorbance (correct)
  • The relationship between temperature and volume
  • The relationship between color and density
  • What is the purpose of a monochromator in a spectrophotometer?

  • To adjust the temperature
  • To amplify the signal
  • To mix different wavelengths
  • To filter out unwanted light (correct)
  • Which type of sample is unsuitable for analysis using a spectrophotometer?

    <p>Opaque</p> Signup and view all the answers

    What is the relationship between absorbance and concentration in spectrophotometry?

    <p>Directly proportional</p> Signup and view all the answers

    What is one function that a spectrophotometer cannot perform?

    <p>Determine sample temperature</p> Signup and view all the answers

    Study Notes

    Absorbance

    • In spectrophotometry, a reference cuvette is used to calibrate the instrument.
    • The Beer-Lambert law describes the relationship between light intensity and absorbance.
    • A monochromator in a spectrophotometer filters out unwanted light.
    • Opaque samples are unsuitable for analysis using a spectrophotometer.
    • Absorbance and concentration have a direct proportional relationship in spectrophotometry.

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    Description

    Test your understanding of spectrophotometry concepts, including the purpose of reference cuvettes and the Beer-Lambert law.

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