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Spectrophotometry and Chromatography Basics

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Spectrophotometry and Chromatography Basics

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Questions and Answers

What is the purpose of a photomultiplier tube in a spectrophotometer?

To split light into different wavelengths

To amplify the signal (correct)

To measure temperature

To generate light

How does a spectrophotometer detect the amount of light absorbed by a sample?

By measuring the light scattered by the sample

By measuring the light reflected off the sample

By measuring the light transmitted through the sample (correct)

By measuring the light emitted by the sample

What is the role of a detector in a spectrophotometer?

To convert light into an electrical signal (correct)

To heat the sample

To adjust the wavelength

To analyze the color of the sample

Which type of spectrophotometer is used to analyze the interaction between light and matter?

UV-Vis spectrophotometer Signup and view all the answers

What is chromatography primarily used for?

Separating and analyzing mixtures Signup and view all the answers

Which component in a spectrophotometer splits light into different wavelengths?

Monochromator Signup and view all the answers

Study Notes

Spectrophotometry

The purpose of a photomultiplier tube in a spectrophotometer is to amplify the signal.
A spectrophotometer detects the amount of light absorbed by a sample by measuring the light transmitted through the sample.
The role of a detector in a spectrophotometer is to convert light into an electrical signal.

Types of Spectrophotometers

UV-Vis spectrophotometer is used to analyze the interaction between light and matter.

Chromatography

Chromatography is primarily used for separating and analyzing mixtures.

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Description

Learn about the principles and components of spectrophotometers, including photomultiplier tubes and detectors, as well as the basics of chromatography.