31 Questions
What is the main purpose of gel electrophoresis?
To separate different components of a mixture based on their size and charge
Which molecules are typically separated using gel electrophoresis?
Strands of DNA, RNA, or different proteins
What properties are taken advantage of in gel electrophoresis experiments?
Size, charge, and shape
Which biochemical techniques are primarily developed to diagnose illnesses and abnormalities?
ELISA, radioimmunoassay, blotting, PCR, cell culture, hybridoma, and cloning processes
What is the purpose of applying an electric field across the gel in gel electrophoresis?
To make the molecules travel through the gel
Which property determines the movement of molecules during gel electrophoresis?
Size and charge
In SDS-PAGE, what does sodium dodecyl sulfate (SDS) do to the proteins?
It denatures the proteins and adds negative charges
Why do smaller strings travel faster than longer strings in SDS-PAGE?
Because smaller strings are less aerodynamic
What is the main factor that determines the speed at which different strands of DNA and RNA move?
Size and shape
Why is protein separation more complicated than nucleic acid in types of SDS-PAGE?
Due to differences in charge distribution and 3D shape of proteins
What effect does the presence of disulfide bonds have on the speed at which a protein travels in Reducing SDS-PAGE?
It decreases the speed of protein travel
What is the main factor that determines how quickly different proteins move in SDS-PAGE?
Both charge distribution and size and shape
Why does the larger protein travel faster in nucleic acid separation despite being more negatively charged?
The negative charge density is spread out more
What does the addition of SDS to proteins achieve before running them on the gel in SDS-PAGE?
It denatures the proteins and adds negative charges
What is one reason for using SDS-PAGE to separate proteins just by their size?
To eliminate differences in charge distribution and 3D shape
Gel electrophoresis separates different components of a mixture based on their size and charge, but not shape.
False
The main purpose of gel electrophoresis is to separate proteins based on their charge.
False
ELISA and PCR are primarily developed to diagnose illnesses and abnormalities.
True
The presence of disulfide bonds has no effect on the speed at which a protein travels in Reducing SDS-PAGE.
False
Chromatography is used to gain knowledge of biomolecules such as proteins, lipids, carbohydrates, and nucleic acids.
True
Spectrophotometry is mainly used to diagnose illnesses and malfunctions.
False
Proteins with a higher negative charge will always travel towards the positively charged anode more quickly in gel electrophoresis.
False
In gel electrophoresis, charge density is the only factor that determines the speed at which different proteins move.
False
SDS-PAGE is primarily used to separate proteins based on their charge distribution and 3D shape.
False
Gel electrophoresis can be used to separate molecules based solely on their aerodynamics.
False
In reducing SDS-PAGE, the presence of disulfide bonds generally increases the speed at which a protein travels through the gel.
False
In SDS-PAGE, the addition of SDS causes proteins to maintain their natural 3D structure during the separation process.
False
Gel electrophoresis is primarily used for diagnosing illnesses and abnormalities in biochemical techniques.
False
The primary purpose of gel electrophoresis is to separate molecules based solely on their size.
False
Nucleic acid separation is solely dependent on charge, not affected by factors such as size and shape.
False
Proteins with fewer negative side chains will always travel faster in SDS-PAGE due to their smaller size.
False
Explore the fundamental biochemical techniques used to study biomolecules and their functions, including spectrophotometry, chromatography, ELISA, radioimmunoassay, blotting, PCR, and cell culture. Gain insight into the recent progress in understanding gene structure and expression.
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