Introduction to Biochemistry Lab Techniques
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Questions and Answers

What is the main purpose of gel electrophoresis?

  • To combine different components of a mixture based on their size and charge
  • To gain knowledge of gene structure and expression
  • To separate different components of a mixture based on their size and charge (correct)
  • To analyze the biochemical techniques such as spectrophotometry and chromatography

Which molecules are typically separated using gel electrophoresis?

  • Malfunctions and abnormalities
  • Strands of DNA, RNA, or different proteins (correct)
  • Nucleic acids and spectrophotometry
  • Carbohydrates and lipids

What properties are taken advantage of in gel electrophoresis experiments?

  • Size, charge, and shape (correct)
  • Temperature, pressure, and volume
  • Color, texture, and weight
  • Density, viscosity, and concentration

Which biochemical techniques are primarily developed to diagnose illnesses and abnormalities?

<p>ELISA, radioimmunoassay, blotting, PCR, cell culture, hybridoma, and cloning processes (A)</p> Signup and view all the answers

What is the purpose of applying an electric field across the gel in gel electrophoresis?

<p>To make the molecules travel through the gel (A)</p> Signup and view all the answers

Which property determines the movement of molecules during gel electrophoresis?

<p>Size and charge (A)</p> Signup and view all the answers

In SDS-PAGE, what does sodium dodecyl sulfate (SDS) do to the proteins?

<p>It denatures the proteins and adds negative charges (C)</p> Signup and view all the answers

Why do smaller strings travel faster than longer strings in SDS-PAGE?

<p>Because smaller strings are less aerodynamic (C)</p> Signup and view all the answers

What is the main factor that determines the speed at which different strands of DNA and RNA move?

<p>Size and shape (D)</p> Signup and view all the answers

Why is protein separation more complicated than nucleic acid in types of SDS-PAGE?

<p>Due to differences in charge distribution and 3D shape of proteins (B)</p> Signup and view all the answers

What effect does the presence of disulfide bonds have on the speed at which a protein travels in Reducing SDS-PAGE?

<p>It decreases the speed of protein travel (A)</p> Signup and view all the answers

What is the main factor that determines how quickly different proteins move in SDS-PAGE?

<p>Both charge distribution and size and shape (A)</p> Signup and view all the answers

Why does the larger protein travel faster in nucleic acid separation despite being more negatively charged?

<p>The negative charge density is spread out more (B)</p> Signup and view all the answers

What does the addition of SDS to proteins achieve before running them on the gel in SDS-PAGE?

<p>It denatures the proteins and adds negative charges (C)</p> Signup and view all the answers

What is one reason for using SDS-PAGE to separate proteins just by their size?

<p>To eliminate differences in charge distribution and 3D shape (C)</p> Signup and view all the answers

Gel electrophoresis separates different components of a mixture based on their size and charge, but not shape.

<p>False (B)</p> Signup and view all the answers

The main purpose of gel electrophoresis is to separate proteins based on their charge.

<p>False (B)</p> Signup and view all the answers

ELISA and PCR are primarily developed to diagnose illnesses and abnormalities.

<p>True (A)</p> Signup and view all the answers

The presence of disulfide bonds has no effect on the speed at which a protein travels in Reducing SDS-PAGE.

<p>False (B)</p> Signup and view all the answers

Chromatography is used to gain knowledge of biomolecules such as proteins, lipids, carbohydrates, and nucleic acids.

<p>True (A)</p> Signup and view all the answers

Spectrophotometry is mainly used to diagnose illnesses and malfunctions.

<p>False (B)</p> Signup and view all the answers

Proteins with a higher negative charge will always travel towards the positively charged anode more quickly in gel electrophoresis.

<p>False (B)</p> Signup and view all the answers

In gel electrophoresis, charge density is the only factor that determines the speed at which different proteins move.

<p>False (B)</p> Signup and view all the answers

SDS-PAGE is primarily used to separate proteins based on their charge distribution and 3D shape.

<p>False (B)</p> Signup and view all the answers

Gel electrophoresis can be used to separate molecules based solely on their aerodynamics.

<p>False (B)</p> Signup and view all the answers

In reducing SDS-PAGE, the presence of disulfide bonds generally increases the speed at which a protein travels through the gel.

<p>False (B)</p> Signup and view all the answers

In SDS-PAGE, the addition of SDS causes proteins to maintain their natural 3D structure during the separation process.

<p>False (B)</p> Signup and view all the answers

Gel electrophoresis is primarily used for diagnosing illnesses and abnormalities in biochemical techniques.

<p>False (B)</p> Signup and view all the answers

The primary purpose of gel electrophoresis is to separate molecules based solely on their size.

<p>False (B)</p> Signup and view all the answers

Nucleic acid separation is solely dependent on charge, not affected by factors such as size and shape.

<p>False (B)</p> Signup and view all the answers

Proteins with fewer negative side chains will always travel faster in SDS-PAGE due to their smaller size.

<p>False (B)</p> Signup and view all the answers

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