10 Questions
In HPLC, what is the term used for the ability to collect a fraction of the eluate containing a purified compound?
Preparative chromatography
What type of detectors are most commonly employed in UV/V spectrophotometers?
Diode array detectors
Which type of detectors may also be used in addition to diode array detectors in spectrophotometry?
Electrochemical detectors
What is done with the mobile phase after it exits the detector in chromatography?
Sent to waste
In HPLC, what does the term 'eluate' refer to?
Compounds separated by the column
What is the purpose of using a detector in HPLC?
To measure the concentration of compounds in the mobile phase
What term is used to describe the process of collecting a purified compound from the eluate in HPLC?
Preparative chromatography
What is the purpose of using a differential refractometer as a detector in HPLC?
To measure the difference in refractive index between the mobile phase and the sample
What happens to the mobile phase after it exits the detector in HPLC?
It is sent to waste or collected, as desired
Which of the following detectors is NOT mentioned in the text as being potentially used in HPLC?
Infrared detector
Learn about different types of detectors used in High Performance Liquid Chromatography (HPLC) including UV/V is spectrophotometers, fluorescence spectrophotometers, and mass spectrometers. Explore how compounds are detected and separated with HPLC.
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