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High Performance Liquid Chromatography Detectors

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High Performance Liquid Chromatography Detectors

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Questions and Answers

In HPLC, what is the term used for the ability to collect a fraction of the eluate containing a purified compound?

Explanatory chromatography

Descriptive chromatography

Analytical chromatography

Preparative chromatography (correct)

What type of detectors are most commonly employed in UV/V spectrophotometers?

Diode array detectors (correct)

Mass spectrometers

Differential refractometers

Fluorescence detectors

Which type of detectors may also be used in addition to diode array detectors in spectrophotometry?

Radioactivity detectors

Light scattering detectors

Differential refractometers

Electrochemical detectors (correct)

What is done with the mobile phase after it exits the detector in chromatography?

Sent to waste Signup and view all the answers

In HPLC, what does the term 'eluate' refer to?

Compounds separated by the column Signup and view all the answers

What is the purpose of using a detector in HPLC?

To measure the concentration of compounds in the mobile phase Signup and view all the answers

What term is used to describe the process of collecting a purified compound from the eluate in HPLC?

Preparative chromatography Signup and view all the answers

What is the purpose of using a differential refractometer as a detector in HPLC?

To measure the difference in refractive index between the mobile phase and the sample Signup and view all the answers

What happens to the mobile phase after it exits the detector in HPLC?

It is sent to waste or collected, as desired Signup and view all the answers

Which of the following detectors is NOT mentioned in the text as being potentially used in HPLC?

Infrared detector Signup and view all the answers