High Performance Liquid Chromatography Detectors
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Questions and Answers

In HPLC, what is the term used for the ability to collect a fraction of the eluate containing a purified compound?

  • Explanatory chromatography
  • Descriptive chromatography
  • Analytical chromatography
  • Preparative chromatography (correct)
  • What type of detectors are most commonly employed in UV/V spectrophotometers?

  • Diode array detectors (correct)
  • Mass spectrometers
  • Differential refractometers
  • Fluorescence detectors
  • Which type of detectors may also be used in addition to diode array detectors in spectrophotometry?

  • Radioactivity detectors
  • Light scattering detectors
  • Differential refractometers
  • Electrochemical detectors (correct)
  • What is done with the mobile phase after it exits the detector in chromatography?

    <p>Sent to waste</p> Signup and view all the answers

    In HPLC, what does the term 'eluate' refer to?

    <p>Compounds separated by the column</p> Signup and view all the answers

    What is the purpose of using a detector in HPLC?

    <p>To measure the concentration of compounds in the mobile phase</p> Signup and view all the answers

    What term is used to describe the process of collecting a purified compound from the eluate in HPLC?

    <p>Preparative chromatography</p> Signup and view all the answers

    What is the purpose of using a differential refractometer as a detector in HPLC?

    <p>To measure the difference in refractive index between the mobile phase and the sample</p> Signup and view all the answers

    What happens to the mobile phase after it exits the detector in HPLC?

    <p>It is sent to waste or collected, as desired</p> Signup and view all the answers

    Which of the following detectors is NOT mentioned in the text as being potentially used in HPLC?

    <p>Infrared detector</p> Signup and view all the answers

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