Skin Tumors Quiz
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Questions and Answers

Explain the etiology of squamous cell carcinoma (SCC) of the skin.

SCC of the skin is primarily caused by exposure to ultraviolet radiation (UVR), such as sunlight and tanning. Other factors include older age, history of excessive sun exposure, phototherapy, and occupational exposure, particularly in outdoor workers like farmers.

What is the incidence of SCC in the Continental United States?

12 per 100,000 white men; 7 per 100,000 white women.

What role does human papillomavirus (HPV) play in the development of squamous cell carcinoma?

Human papillomavirus, particularly the oncogenic types, can contribute to the development of SCC.

Who is more susceptible to the development of SCC, males or females?

<p>Males are more susceptible than females.</p> Signup and view all the answers

What are the occupational risk factors associated with the development of SCC?

<p>Persons working outdoors, particularly farmers, are at higher risk of developing SCC due to prolonged sun exposure.</p> Signup and view all the answers

Describe the steps of Gram staining a skin specimen.

<p>The steps of Gram staining a skin specimen involve applying crystal violet, iodine, alcohol, and safranin to the specimen, followed by observation under a microscope.</p> Signup and view all the answers

Interpret the findings of a KOH Mount.

<p>The findings of a KOH Mount involve observing the specimen under a microscope for the presence of fungal elements, such as hyphae and spores.</p> Signup and view all the answers

Describe the setting up of a fungal culture.

<p>Setting up a fungal culture involves inoculating the specimen onto appropriate fungal culture media, such as Sabouraud dextrose agar, and then incubating it at the appropriate temperature.</p> Signup and view all the answers

Describe the collection and transport of anaerobic samples.

<p>The collection of anaerobic samples involves using a syringe and needle to aspirate the specimen into a sterile container, which is then transported anaerobically to the laboratory.</p> Signup and view all the answers

Describe the handling of anaerobic specimen in the laboratories.

<p>The handling of anaerobic specimens in the laboratories involves processing the samples in an anaerobic chamber or using anaerobic techniques to maintain the viability of anaerobic organisms.</p> Signup and view all the answers

Explain the collection of skin and soft tissue specimen in bacterial infections.

<p>Skin and soft tissue specimens can be collected from open wounds or broken skin. Swabs can be taken from the infected area. However, it is important to note that surface wounds are often colonized with environmental bacteria, and swab samples often do not reflect the true cause of the infectious process.</p> Signup and view all the answers

Describe the steps in the preparation of a KOH mount.

<p>The steps in the preparation of a KOH mount involve placing a small amount of the specimen on a slide, adding a drop of 10-20% potassium hydroxide (KOH), covering it with a coverslip, and examining it under a microscope.</p> Signup and view all the answers

What are the morphological features of common anaerobes as seen on Gram stain?

<p>Common anaerobes as seen on Gram stain may appear as gram-positive or gram-variable rods, cocci, or pleomorphic forms. They may also exhibit various arrangements.</p> Signup and view all the answers

Explain the interpretation of the findings of a gram stain of a skin specimen.

<p>The interpretation of the findings of a gram stain of a skin specimen involves observing the presence and characteristics of bacterial cells, such as their shape, arrangement, and staining properties (gram-positive or gram-negative). This information can help in identifying the potential causative agents of the infection.</p> Signup and view all the answers

Describe the handling of anaerobic specimen in the laboratories.

<p>Anaerobic specimens in the laboratories should be handled carefully to maintain their anaerobic environment. This includes minimizing exposure to oxygen, using appropriate transport media, and ensuring prompt processing to maintain the viability of anaerobic organisms.</p> Signup and view all the answers

Explain the steps for collecting skin and soft tissue specimens in bacterial infections.

<p>The steps for collecting skin and soft tissue specimens in bacterial infections involve swabbing the infected area, but it is important to note that surface wounds are often colonized with environmental bacteria and swab samples may not reflect the true cause of the infectious process. For open wounds or broken skin, swabs can be taken, while for closed wounds or abscesses, different methods may be necessary.</p> Signup and view all the answers

Describe the process of Gram staining a skin specimen.

<p>The process of Gram staining a skin specimen involves the application of crystal violet stain, followed by iodine, alcohol, and safranin. This helps to differentiate and classify bacteria into Gram-positive and Gram-negative categories based on their cell wall structure.</p> Signup and view all the answers

What are the key considerations for interpreting the findings of a Gram stain of a skin specimen?

<p>The key considerations for interpreting the findings of a Gram stain of a skin specimen include observing the color and morphology of the stained bacteria, as well as identifying whether they are Gram-positive or Gram-negative. This information can provide important clues about the nature of the infectious process.</p> Signup and view all the answers

Explain the process for collecting skin specimens in fungal infections.

<p>In fungal infections, the collection of skin specimens involves scraping the infected area to obtain a sample for further analysis. This sample is then used for preparing a KOH mount and setting up a fungal culture to identify the presence of fungi.</p> Signup and view all the answers

Describe the steps involved in preparing a KOH mount for fungal analysis.

<p>The steps involved in preparing a KOH mount for fungal analysis include obtaining a skin specimen, placing it on a slide, adding a drop of 10-20% KOH solution, covering it with a coverslip, and examining it under a microscope. The KOH helps to dissolve human tissue, leaving fungal elements visible for identification.</p> Signup and view all the answers

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