Part 1

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Questions and Answers

What is the recommended maximum depth for a skin puncture on an infant?

2.0 mm (correct)
3.0 mm
2.5 mm
3.5 mm

Why is the first drop of blood discarded during a skin puncture?

To eliminate excess anticoagulant
To prevent hemolysis
To remove tissue fluid contamination (correct)
To ensure adequate sample volume

What is the most common site for venipuncture?

Superficial vein of the antecubital fossa (correct)
Saphenous vein
Femoral vein
Dorsal vein of the hand

What angle should the needle be inserted at during venipuncture?

30 degrees (C) Signup and view all the answers

What is the maximum recommended time for tourniquet application during venipuncture?

1 minute (D) Signup and view all the answers

Prolonged tourniquet application can cause:

Hemoconcentration (B) Signup and view all the answers

Which of the following is the first choice vein for venipuncture, according to the 'N' shaped pattern?

Median cubital vein (B) Signup and view all the answers

Which of the following can result from repeatedly pumping the fist during blood collection?

Increased potassium (D) Signup and view all the answers

Which of the following is a cause of specimen hemolysis?

Prolonged tourniquet application (B) Signup and view all the answers

What is the function of sodium fluoride in blood collection tubes?

To inhibit glycolysis (B) Signup and view all the answers

Which of the following additives is commonly found in a 'Hema' tube?

EDTA (D) Signup and view all the answers

What is the mechanism of action of EDTA as an anticoagulant?

Chelates calcium (D) Signup and view all the answers

For what duration should a CBC sample, collected in EDTA, be analyzed if stored at room temperature?

Within 6 hours (B) Signup and view all the answers

Following venipuncture, a patient develops a large bruise at the site. What immediate action should be taken?

Apply direct pressure (D) Signup and view all the answers

A patient feels faint during venipuncture. What is the most appropriate first action?

Lower the patient’s head and loosen any tight clothing. (D) Signup and view all the answers

What is the recommended course of action if a patient experiences a seizure during venipuncture?

Stop the extraction and protect the patient from injury (B) Signup and view all the answers

How does cortisol level fluctuation during the day?

Decrease ACTH, Decrease Cortisol (A) Signup and view all the answers

How does lipemia affect blood test results?

Interfere with certain assays (B) Signup and view all the answers

According to the information, which component is used to operate valves and move the sample through the system in hematology analyzers?

Pneumatics (C) Signup and view all the answers

What is the most common methodology used in automated cell counting and sizing?

Electrical impedance (C) Signup and view all the answers

In electrical impedance, what does the size (height) of the pulse correlate to?

Volume of cells (C) Signup and view all the answers

Which parameter is NOT directly measured by the electronic cell counting?

Hemoglobin (A) Signup and view all the answers

What is the normal value of the Mean Platelet Volume (MPV)?

6.5 - 12 fL (C) Signup and view all the answers

A WBC count is considered valid in electronic cell counting if the count is at least how many fL?

45 fL (B) Signup and view all the answers

In an RBC histogram generated by an automated cell counter, approximately what range (in fL) corresponds to the normal volume range?

36-360 fL (B) Signup and view all the answers

Which type of specimen nature can be counted falsely elavated WBC?

Abnormal RBC (D) Signup and view all the answers

What is the primary method for identifying different cell populations using flow cytometry?

Monoclonal antibodies (D) Signup and view all the answers

Which lineage is associated with the markers CD34, CD117, and TDT?

Immature (C) Signup and view all the answers

Which of the following markers is NOT associated with T Lymphocytes?

CD19 (D) Signup and view all the answers

Flashcards

Skin Puncture

Obtaining small quantities of blood via puncturing the skin.

Skin Puncture Devices

Capillary tubes and microcollection tubes are used.