Mastering Blood Collection Methods
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Questions and Answers

Which method of blood collection is commonly used to obtain capillary blood?

  • Venipuncture
  • Arterial puncture
  • Microtechniques
  • Skin puncture (correct)

    • What is the usual site for skin puncture in adults?

  • Tip of the 1st or 2nd finger
  • Antecubital fossa
  • Tip of the 3rd or 4th finger (correct)
  • Earlobe

    • Which method of blood collection is essential for tests that require anticoagulation and when a large quantity of blood is needed?

  • Venipuncture (correct)
  • Skin puncture
  • Arterial puncture
  • Microtechniques

    • What is the purpose of wiping off the first drop of blood during skin puncture?

    <p>To prevent contamination</p> Signup and view all the answers

    Which method of blood collection is not included in current medical technology practice in the Philippines?

    <p>Arterial puncture</p> Signup and view all the answers

    What can be done to relieve difficulty in obtaining blood during skin puncture?

    <p>Putting warm compress on the site</p> Signup and view all the answers

    Which veins are usually used for obtaining venous blood?

    <p>The median cubital, the cephalic, and the basilic veins</p> Signup and view all the answers

    What are the two methods for performing venipuncture?

    <p>The syringe method and the evacuated tube method</p> Signup and view all the answers

    What is the recommended method for the whole blood clotting time technique?

    <p>The two-syringe method</p> Signup and view all the answers

    What is the purpose of a central venous access device (CVAD)?

    <p>To access the bloodstream for the delivery of therapies</p> Signup and view all the answers

    What are the two types of CVAD available?

    <p>Catheter and port</p> Signup and view all the answers

    What is the push-pull method used for in blood collection from CVAD?

    <p>To collect the required amount of blood</p> Signup and view all the answers

    Which of the following is the correct order of draw for evacuated tubes used in a multi-draw collection?

    <p>Lavender, Green, Red</p> Signup and view all the answers

    What is the correct angle of insertion for arterial puncture?

    <p>35°</p> Signup and view all the answers

    What is the recommended time to apply pressure on the puncture site after arterial puncture?

    <p>5 minutes</p> Signup and view all the answers

    What is the main disadvantage of skin puncture?

    <p>Limited blood volume</p> Signup and view all the answers

    What are the causes of hematoma?

    <p>All of the above</p> Signup and view all the answers

    What is hemoconcentration?

    <p>Increased concentration of red blood cells in the blood</p> Signup and view all the answers

    What is thrombophlebitis?

    <p>Formation of blood clots in the veins</p> Signup and view all the answers

    What is a hematoma?

    <p>Collection of blood in the tissues</p> Signup and view all the answers

    Which of the following is the correct measurement of each tertiary square in the central secondary square?

    <p>0.2 mm</p> Signup and view all the answers

    How many smaller squares are there in each tertiary square?

    <p>16</p> Signup and view all the answers

    What is the total number of smaller squares in the central secondary square?

    <p>400</p> Signup and view all the answers

    How many tertiary squares are counted for RBs?

    <p>5</p> Signup and view all the answers

    What is the total number of smaller squares counted for RBs?

    <p>80</p> Signup and view all the answers

    What is the purpose of a thick coverslip in the counting chamber?

    <p>To create a space between the coverslip and the platform</p> Signup and view all the answers

    How is the Neubauer counting chamber different from the Improved Neubauer counting chamber?

    <p>The Neubauer counting chamber has a smaller ruled area</p> Signup and view all the answers

    What is the principal use of the Fuchs-Rosenthal counting chamber?

    <p>To perform low cell count tests</p> Signup and view all the answers

    How many squares are there in each ruled area of the Speirs-Levy counting chamber?

    <p>10</p> Signup and view all the answers

    What is the depth of the Speirs-Levy counting chamber?

    <p>0.2 mm</p> Signup and view all the answers

    Which type of blood cells can be counted using a hemacytometer?

    <p>Red blood cells (RBCs), white blood cells (WBCs), and platelets</p> Signup and view all the answers

    What are the basic parts of a hemacytometer?

    <p>Counting chamber, WBC pipette, RBC pipette, suction device, and thick coverslip</p> Signup and view all the answers

    What is the purpose of the improved Neubauer counting chamber?

    <p>To count both red blood cells (RBCs) and white blood cells (WBCs)</p> Signup and view all the answers

    What is the ruling pattern of the improved Neubauer counting chamber?

    <p>Secondary squares measuring 1x1 mm</p> Signup and view all the answers

    Which secondary squares of the improved Neubauer counting chamber are used for white blood cell (WBC) count?

    <p>W1, W2, W3, and W4</p> Signup and view all the answers

    What is the material used to make the improved Neubauer counting chamber?

    <p>Heavy colorless glass</p> Signup and view all the answers

    How many parallel platforms are there on the improved Neubauer counting chamber?

    <p>Three</p> Signup and view all the answers

    What is the depth difference between the lateral platforms and the central platform of the improved Neubauer counting chamber?

    <p>0.1 mm</p> Signup and view all the answers

    What is the purpose of the transverse groove on the central platform of the improved Neubauer counting chamber?

    <p>To divide the central platform into halves</p> Signup and view all the answers

    What is the area of the primary square on the improved Neubauer counting chamber?

    <p>9 mm²</p> Signup and view all the answers

    Which part of the counting chamber holds the glass cover slip at 0.1 mm above the surface of the counting grid?

    <p>The side ridges of the chamber</p> Signup and view all the answers

    What is the area of each big square in the counting grid?

    <p>9 mm^2</p> Signup and view all the answers

    What is the area of the red square in the counting grid?

    <p>1 mm^2</p> Signup and view all the answers

    How many small squares are there in each corner square of the counting grid?

    <p>16</p> Signup and view all the answers

    What is the volume of diluted blood in 4 small squares of the counting grid?

    <p>0.4 mm^3</p> Signup and view all the answers

    What is the area of each small corner square in the central square of the counting grid?

    Signup and view all the answers

    How many smallest squares are there in each smaller square of the central square of the counting grid?

    <p>16</p> Signup and view all the answers

    What is the volume of diluted blood in 5 small squares of the counting grid?

    <p>0.02 mm^3</p> Signup and view all the answers

    What is the purpose of the rolling bead in the bulb of the diluting pipettes?

    <p>To mix the blood with diluting fluids</p> Signup and view all the answers

    What is the difference in calibrations between the RBC and WBC pipettes?

    <p>0.5, 1.0 &amp; 11 for RBC and 0.5, 1.0 &amp; 101 for WBC</p> Signup and view all the answers

    Which of the following is the dilution factor for WBC counting in the improved Neubauer counting chamber?

    <p>20</p> Signup and view all the answers

    What is the dilution factor for RBC counting in the improved Neubauer counting chamber?

    <p>200</p> Signup and view all the answers

    What is the recommended magnification for WBC counting in the improved Neubauer counting chamber?

    <p>10X</p> Signup and view all the answers

    What is the recommended magnification for RBC counting in the improved Neubauer counting chamber?

    <p>40X</p> Signup and view all the answers

    What is the counting rule for cells in the improved Neubauer counting chamber?

    <p>Count the cells touching the triple lines of the left side and on the top of the square in the same square. Count the cells touching Bottom line, Right line in adjacent square.</p> Signup and view all the answers

    What is the ideal charging of the chamber in the improved Neubauer counting chamber?

    <p>The chamber should be completely filled with diluted blood over the counting grid under the cover slip.</p> Signup and view all the answers

    What is the consequence of over charging the chamber in the improved Neubauer counting chamber?

    <p>Diluted blood flows into the side grooves.</p> Signup and view all the answers

    What is the consequence of under charging the chamber in the improved Neubauer counting chamber?

    <p>The diluted blood drop is insufficient to cover the counting grid or any air bubble.</p> Signup and view all the answers

    How many tertiary squares are counted for WBCs in the improved Neubauer counting chamber?

    <p>9</p> Signup and view all the answers

    How many tertiary squares are counted for RBCs in the improved Neubauer counting chamber?

    <p>16</p> Signup and view all the answers

    Which of the following is the correct definition of hemocytometry?

    <p>A technique used to count the total number of cells in the blood or other biological body fluids</p> Signup and view all the answers

    What is the purpose of hemocytometry?

    <p>To find out normal and abnormal count of the cells</p> Signup and view all the answers

    What is the principle of cell counting in hemocytometry?

    <p>The blood sample is diluted to a known degree and then the cells are counted under a microscope</p> Signup and view all the answers

    What is a hemocytometer?

    <p>A special type of microscope slide used for counting cells in blood or fluid</p> Signup and view all the answers

    Who invented the hemocytometer?

    <p>Louis-Charles Malassez</p> Signup and view all the answers

    What is the correct calculation to determine the number of cells in undiluted blood using hemocytometry?

    <p>Number of cells counted in diluted blood / Dilution factor</p> Signup and view all the answers

    What is the unit of measurement for cell count in hemocytometry?

    <p>Cells per cubic mm</p> Signup and view all the answers

    What can be determined by counting the cells in the blood or body fluids?

    <p>If an individual is normal or not</p> Signup and view all the answers

    How is the blood sample prepared for cell counting using a hemocytometer?

    <p>The blood sample is diluted in a special pipette and the diluted blood drop is then placed under a cover slip on a counting chamber</p> Signup and view all the answers

    What is the difference between a hemocytometer and an electronic cell counter?

    <p>A hemocytometer is used for manual cell counting under a microscope, while an electronic cell counter automatically counts the cells</p> Signup and view all the answers

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