10 Questions
What are the differences between cationic and anionic stains?
Cationic stains have a positive charge associated with them, while anionic stains carry a negative charge.
Give examples of cationic stains and anionic stains mentioned in the text.
Examples of cationic stains include crystal violet, safranin, basic fuschin, & methylene blue. Examples of anionic stains include eosin, nigrosin, & congo red.
What are the characteristics of cationic stains and where are they excellent in binding?
Cationic stains have a positive charge associated with them and are excellent in binding with negatively charged molecular structures such as bacterial cell walls and lifeinte.
What are the characteristics of anionic stains and what are they often used for?
Anionic stains carry a negative charge and are often used to stain the slide background, which leaves the microbe transparent.
What is the process for removing excess stains and allowing better viewing of the cells?
Stains require a short exposure time to their target followed by a brief, light rinse with deionized (DI) water to remove any excess stain and allow better viewing of the cells that carry the stain.
What is the main difference between cationic and anionic stains?
Cationic stains have a positive charge, while anionic stains carry a negative charge
What are examples of cationic stains mentioned in the text?
Crystal violet, safranin, basic fuchsin, & methylene blue
What are acid dyes often used for in staining?
Staining the slide background
What is the process for removing excess stains and allowing better viewing of the cells?
Short exposure time to the target followed by a brief, light rinse with deionized water
What are examples of anionic stains mentioned in the text?
Eosin, nigrosin, & congo red
Test your knowledge of simple staining techniques with this quiz on the differences between positively and negatively charged stains, their mechanisms of action, and the factors influencing their absorption of light.
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