Preparation of Specimen for Light Microscopy and Staining Techniques

To highlight the entire microorganism so that cellular shapes and basic structures are visible.

Gram-positive bacteria retain the crystal violet stain and appear purple, while gram-negative bacteria do not retain the crystal violet stain and appear pink after counterstaining with safranin.

Mordants increase the affinity of a stain for a biological specimen.

<p>Acid Fast Staining is used to identify Mycobacterium species and some species of Nocardia.</p> Signup and view all the answers

<p>Gram-positive bacteria retain the crystal violet stain and appear purple.</p> Signup and view all the answers

<p>Gram-positive bacteria appear purple, while gram-negative bacteria appear pink.</p> Signup and view all the answers

<p>Blue</p> Signup and view all the answers

<p>Hans Christian Gram</p> Signup and view all the answers

<p>To intensify the primary stain</p> Signup and view all the answers

<p>Step 3: Decolorizing agent Alcohol</p> Signup and view all the answers

<p>To give a contrasting color to decolorized bacteria</p> Signup and view all the answers

<p>Purple</p> Signup and view all the answers