Preparation of Specimen for Light Microscopy and Staining Techniques
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Questions and Answers

What is the primary purpose of a simple stain?

To highlight the entire microorganism so that cellular shapes and basic structures are visible.

What is the difference between gram-positive and gram-negative bacteria in Gram Staining?

Gram-positive bacteria retain the crystal violet stain and appear purple, while gram-negative bacteria do not retain the crystal violet stain and appear pink after counterstaining with safranin.

What is the purpose of a mordant in staining?

Mordants increase the affinity of a stain for a biological specimen.

What is the purpose of Acid Fast Staining?

<p>Acid Fast Staining is used to identify Mycobacterium species and some species of Nocardia.</p> Signup and view all the answers

How are gram-positive bacteria visually identified in Gram Staining?

<p>Gram-positive bacteria retain the crystal violet stain and appear purple.</p> Signup and view all the answers

What is the key difference in staining results between gram-positive and gram-negative bacteria?

<p>Gram-positive bacteria appear purple, while gram-negative bacteria appear pink.</p> Signup and view all the answers

What color do non-acid-fast bacteria appear after being treated with methylene blue following staining with carbolfuchsin and acid-alcohol?

<p>Blue</p> Signup and view all the answers

Who developed the Gram stain in 1884?

<p>Hans Christian Gram</p> Signup and view all the answers

What is the purpose of the mordant in the Gram staining process?

<p>To intensify the primary stain</p> Signup and view all the answers

Which step in the Gram staining process involves the use of a decolorizing agent?

<p>Step 3: Decolorizing agent Alcohol</p> Signup and view all the answers

What is the purpose of the counterstain in Gram staining?

<p>To give a contrasting color to decolorized bacteria</p> Signup and view all the answers

What color do gram-positive cells appear after the completion of the Gram staining process?

<p>Purple</p> Signup and view all the answers

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