Shigella Infections Quiz
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Questions and Answers

What is the primary mode of transmission for Shigella infection?

  • Direct contact with skin
  • Airborne transmission
  • Fecal-oral route (correct)
  • Vector-borne transmission
  • Which Shigella species is most commonly found in developing countries?

  • Shigella boydii
  • Shigella dysenteriae
  • Shigella flexneri (correct)
  • Shigella sonnei
  • What is a characteristic symptom of shigellosis?

  • Nausea followed by vomiting
  • Severe abdominal cramps without diarrhea
  • Watery diarrhea followed by dysentery (correct)
  • High fever and rash
  • What is the incubation period for Shigella infections?

    <p>1-3 days (B)</p> Signup and view all the answers

    Which characteristic is NOT true for Shigella species?

    <p>They are motile gram-negative bacteria (B)</p> Signup and view all the answers

    What is the primary cause of mortality in infants due to diarrhea in developing countries?

    <p>Shigella infection (B)</p> Signup and view all the answers

    Which of the following accurately describes the early stage of Shigellosis?

    <p>Watery diarrhea and fever (C)</p> Signup and view all the answers

    What is one of the primary virulence factors of Shigella?

    <p>Invasiveness and adherence to host cells (A)</p> Signup and view all the answers

    What type of diarrhea does Shiga toxin primarily cause in the early stage of infection?

    <p>Watery diarrhea (B)</p> Signup and view all the answers

    Which diagnostic test is indicated for the presence of polymorphonuclear leukocytes (PMNs) in feces?

    <p>Methylene blue staining of feces (C)</p> Signup and view all the answers

    What treatment approach is primarily emphasized for managing Shigella infections?

    <p>Supportive treatment for dehydration (D)</p> Signup and view all the answers

    Which of the following statements about Shiga toxin is correct?

    <p>It has both enterotoxic and cytotoxic properties. (D)</p> Signup and view all the answers

    What enhances the growth of Vibrio cholerae in laboratory settings?

    <p>1% salt addition (A)</p> Signup and view all the answers

    What is the primary route of transmission for cholera?

    <p>Oral fecal route (D)</p> Signup and view all the answers

    What characterizes the stool of a cholera patient?

    <p>Rice-water appearance (A)</p> Signup and view all the answers

    Which virulence determinant is crucial for cholera toxin's effectiveness?

    <p>Bacterial colonization (D)</p> Signup and view all the answers

    What percentage of body weight loss indicates moderate dehydration in cholera?

    <p>Up to 10% (B)</p> Signup and view all the answers

    What type of bacterium is Helicobacter pylori?

    <p>Helical and microaerophilic (C)</p> Signup and view all the answers

    Why is it difficult to develop a vaccine for cholera?

    <p>Unstable strains of Vibrio cholerae (C)</p> Signup and view all the answers

    What does the 'TCP' in TCP pili stand for?

    <p>Toxin-Capture Proteins (C)</p> Signup and view all the answers

    How does Vibrio cholerae tolerate salt in its growth medium?

    <p>Through osmotic regulation (C)</p> Signup and view all the answers

    Which characteristic is NOT true for the Enterobacteriaceae family?

    <p>All species ferment lactose (C)</p> Signup and view all the answers

    Which pairing of disease and bacterium is incorrectly matched?

    <p>Enterobacter - Tuberculosis (C)</p> Signup and view all the answers

    What is a general characteristic of Salmonella?

    <p>H2S producing from thiosulfate (B)</p> Signup and view all the answers

    How are Salmonella species classified according to the Kauffman-White schema?

    <p>Using O and H antigens (D)</p> Signup and view all the answers

    What is the prototype disease for enteric fever caused by Salmonella?

    <p>Typhoid fever (A)</p> Signup and view all the answers

    Which species is categorized as a non-pathogen in the Salmonella genus?

    <p>Salmonella bongori (B)</p> Signup and view all the answers

    Which statement about Salmonella’s biochemical properties is accurate?

    <p>They are resistant to bile salts (D)</p> Signup and view all the answers

    Which of the following Salmonella species is most commonly associated with food poisoning?

    <p>Salmonella enteritidis (A)</p> Signup and view all the answers

    What is the primary result of enteric infections caused by Salmonella?

    <p>Acute gastroenteritis (D)</p> Signup and view all the answers

    Flashcards

    Shigella dysenteriae

    Gram-negative, enteric, facultatively anaerobic rod-shaped prokaryote that causes bacillary dysentery, often found in water contaminated with human feces.

    Shigella

    A genus of bacteria that includes four species: S. sonnei, S.flexneri, S. boydii, and S. dysenteriae. All species can cause bacillary dysentery.

    Bacillary dysentery (Shigellosis)

    A disease caused by Shigella bacteria, characterized by bloody diarrhea, intestinal pain, and fever. It's often referred to as bacillary dysentery.

    Shigella sonnei

    The most common type of Shigella found in the industrial world.

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    Shigella flexneri

    The most common type of Shigella found in developing countries.

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    Enterotoxin

    A type of toxin produced by bacteria that specifically targets the intestines, leading to watery diarrhea and other symptoms.

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    Dysentery

    Inflammation of the intestines, particularly the colon, characterized by severe abdominal cramps, straining to defecate, and bloody, mucus-filled stools.

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    Bacillary dysentery

    A type of dysentery caused by a bacterial infection, often involving invasion of the intestinal lining and production of toxins.

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    Shigellosis

    A bacterial infection characterized by a two-stage disease process:

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    Shiga toxin

    A potent toxin produced by Shigella bacteria, responsible for both enterotoxic (affecting intestines) and cytotoxic (damaging cells) effects. Similar to Shiga-like toxin produced by E. coli.

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    Methylene blue staining

    A diagnostic method for Shigella infections that involves staining stool samples with methylene blue and examining them under a microscope.

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    Supportive treatment

    The primary treatment for Shigellosis, focusing on replacing lost fluids and electrolytes to combat dehydration.

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    What are the general characteristics of Enterobacteriaceae?

    Gram-negative, non-spore-forming bacilli that are facultative anaerobes. They are oxidase negative and reduce nitrates to nitrites. While they all ferment glucose, not all species ferment lactose.

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    What is Shigella?

    A genus of bacteria that includes four species: S. sonnei, S.flexneri, S. boydii, and S. dysenteriae. All species can cause bacillary dysentery.

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    What is bacillary dysentery?

    A disease caused by Shigella bacteria, characterized by bloody diarrhea, intestinal pain, and fever.

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    What is salmonellosis?

    A generic term for any illness caused by Salmonella bacteria, including enteric fever, food poisoning, and septicemia.

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    What are the two species of Salmonella, based on DNA homology?

    Salmonella enterica (six subspecies) and Salmonella bongori

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    What is Shigella sonnei?

    The most common type of Shigella found in the industrial world.

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    What is Shigella flexneri?

    The most common type of Shigella found in developing countries.

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    What is a characteristic feature of the Salmonella bacteria?

    Salmonella - rod prokaryote (dividing); note the flagella. Causes salmonellosis (food poisoning).

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    What is typhoid fever?

    A severe and dangerous form of salmonellosis caused by Salmonella Typhi, characterized by fever, headache, intestinal bleeding, and potential liver damage.

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    V. cholerae

    A specific type of Vibrio bacteria that causes cholera, a severe diarrheal disease. It is characterized by its ability to produce cholera toxin, which disrupts the normal function of intestinal cells, leading to severe dehydration.

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    Cholera toxin

    A toxic protein produced by V. cholerae that binds to intestinal cells, causing them to release large amounts of fluids and electrolytes, leading to severe diarrhea and dehydration.

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    TCP pili

    A type of bacterial adhesion, a protein found on the surface of V. cholerae, that helps the bacteria attach to the lining of the small intestine, facilitating colonization and toxin production.

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    Bacterial colonization

    The ability of V. cholerae to multiply and establish itself within the intestinal tract. This is essential for the bacterium to produce enough cholera toxin to cause severe disease.

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    Dehydration

    A state of severe fluid loss, often due to cholera infection, characterized by sunken eyes, loss of skin elasticity, and extreme weakness.

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    Rice-water stool

    A type of diarrhea associated with cholera, characterized by large volumes of watery, rice-like stools.

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    Rehydration therapy

    A type of treatment strategy for cholera and other diarrheal diseases that involves providing fluids and electrolytes orally or intravenously to replace lost fluids and prevent dehydration.

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    Prevention of cholera

    An important strategy for preventing cholera, based on maintaining a healthy environment, access to clean water, and sanitation practices.

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    Cholera vaccine

    A type of intervention used to protect against cholera, involving the administration of a vaccine that stimulates the immune system to fight the disease. But, there are several constraints to effective vaccination.

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    Study Notes

    Gram-Negative Rods: Enterobacteriaceae & Vibrio

    • Gram-negative bacteria are rod-shaped.
    • Enterobacteriaceae and Vibrio are families of gram-negative rods.
    • The diagram shows a labeled cross-section of the digestive tract. It includes organs like the pharynx, oral cavity, esophagus, stomach, liver, gallbladder, pancreas, small intestine, large intestine (colon, cecum, appendix), rectum, and anus.

    Shigella

    • Shigella are gram-negative, nonmotile, facultative anaerobes.
    • Four species are discussed: Shigella sonnei, Shigella flexneri, Shigella boydii, and Shigella dysenteriae.
    • Shigella sonnei is most common in developed countries, and Shigella flexneri is more common in developing countries.
    • Shigella are non-lactose fermenting and do not produce gas from glucose.
    • They are resistant to bile salts.
    • Shigella dysenteriae is a prokaryote, enteric, and facultatively anaerobic rod, frequently found in water contaminated by human feces.
    • Shigella cause bacillary dysentery or shigellosis, characterized by bloody feces and abdominal pain.
    • The infection invades the intestinal epithelium but doesn't penetrate.
    • This leads to dysentery symptoms appearing 2-3 days post-infection, with mucus, blood, and leukocytes (pus) in the stool.
    • Watery diarrhea can occur alongside dysentery.
    • Shigella have 4 groups according to O antigens: A, B, C, and D.
    • Transmission occurs via the fecal-oral route.
    • Clinical syndromes include shigellosis and its two stages (early and second).

    Salmonella

    • Salmonella are gram-negative, motile, facultative anaerobes, non-lactose fermenting, and resistant to bile salts.
    • They produce hydrogen sulfide (H₂S) from thiosulfate.
    • Salmonella can produce acid only or acid and gas.
    • Salmonella causes salmonellosis, a generic term for disease that can include enteric fever (typhoid fever, less severe paratyphoid fever), salmonella food poisoning, septicemia, and asymptomatic carriage.
    • The incubation period for gastroenteritis (acute gastroenteritis) is 12 hours-3 days and 10 to 14 days for enteric fever.
    • Over 2400 O-serotypes (species) based on Kauffman-White antigenic schema.
    • Biochemical tests assist in classification.
    • Salmonella are rod-shaped prokaryotes and cause salmonellosis, commonly from food contamination.
    • Transmission is fecal-oral.
    • Important in understanding taxonomy and the clinical syndromes of Salmonella infection.

    Vibrio Cholerae

    • Vibrio cholerae is a Gram-negative, curved, motile rod.
    • It's sensitive to low pH and tolerant to salt, typically found in aquatic habitats.
    • Transmitted through the oral-fecal route.
    • High infective dose is needed to cause infection.
    • Cyclic epidemics are possible.
    • Diseases caused by various types of Vibrio cholerae are characterized through mapping water supplies, which leads to identifying and fixing the source of the infection.

    Helicobacter Pylori

    • Helicobacter pylori is a curved gram-negative bacterium
    • It's microaerophilic and acid-tolerant.
    • It produces urease, lives in the mucus and epithelial surfaces of the human stomach.
    • Its genome size is 1.7 MB
    • It is associated with conditions like asymptomatic colonization, chronic atrophic gastritis, peptic ulcer disease, gastric adenocarcinoma, and lymphoma.
    • Transmission occurs through oral ingestion.
    • Historically, Dr. Robin Warren and Dr. Barry Marshall successfully cultured H. pylori, which later earned them a Nobel Prize in 2005.
    • Non-invasive tests like serology, urea breath, and fecal antigen tests can detect H. pylori.
    • Invasive tests like endoscopy-based histologic examination (biopsy), urease test and culture can also be performed.

    Pseudomonas Aeruginosa

    • Pseudomonas aeruginosa is an opportunistic pathogen in people, plants, insects, etc.
    • It's a Gram-negative rod found in water and oxygen environments.
    • Important clinical manifestations include colonization (initial site), severe and disseminated conditions (like pneumonia, cellulitis, etc.).
    • Pathogenesis is complex and includes virulence factors like alginate, LPS, flagella, pili, and secreted exotoxins (like exotoxin A, elastases, etc.).
    • It has antimicrobial resistance mechanisms like chromosomal and plasmid-mediated multi-drug efflux pumps.
    • Biofilm formation by these bacteria is vital to understanding their success and causing disease.

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    Description

    Test your knowledge on Shigella infections and their characteristics. This quiz covers key topics such as transmission modes, common species in developing countries, symptoms of shigellosis, and incubation periods. Perfect for students learning about microbiology and infectious diseases.

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