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Separation Mechanisms in Chromatography

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Questions and Answers

Partitioning is a separation mechanism in GC and LC.

True

SPE is widely used in environmental analysis, food testing, and forensic toxicology.

True

Positive pole at electrophoresis is cathode.

False

HPLC is characterized by high resolution power.

<p>True</p> Signup and view all the answers

Chromatography is basically a group of methods based on the absorption of light by a substance.

<p>False</p> Signup and view all the answers

Study Notes

Separation Mechanisms

Partitioning is a separation mechanism used in Gas Chromatography (GC) and Liquid Chromatography (LC)

Solid-Phase Extraction (SPE)

SPE is widely used in environmental analysis, food testing, and forensic toxicology

High-Performance Liquid Chromatography (HPLC)

HPLC is characterized by high resolution power
HPLC provides separation through high pressure created by small particles at the column (not low pressure)

Electrophoresis

One advantage of electrophoresis is that it has very high resolution
In electrophoresis, the positive pole is an anode (not cathode)
Adding coloring to a sample can slow down confirmation at gel electrophoresis

Spectrophotometry

The proportion of light absorbed depends on the number of molecules it interacts with in a spectrophotometer

Chromatography

Chromatography is a group of methods based on the separation of substances, not on the absorption of light
Chromatography can be used to separate the components of ink

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Description

This quiz covers different separation mechanisms used in chromatography, including partitioning, solid-phase extraction, high-performance liquid chromatography, and electrophoresis.