5 Questions
Which type of ELISA involves the direct immobilization of the antigen onto the solid phase?
Direct ELISA
What is the characteristic feature of Sandwich ELISA that enhances both specificity and sensitivity?
Use of a capture antibody and a detection antibody
What is crucial for maintaining optimal pH and stability throughout the ELISA process?
Bufffer, such as PBS or TBS
What is the purpose of utilizing a two-step process in Indirect ELISA?
A primary antibody binds to the immobilized antigen and a secondary enzyme-conjugated antibody is added
What is the purpose of coating the solid phase in the initial step of the ELISA process?
To immobilize the target antigen or antibody of interest
Study Notes
ELISA Types
- Direct ELISA involves the direct immobilization of the antigen onto the solid phase.
Sandwich ELISA
- The characteristic feature of Sandwich ELISA that enhances both specificity and sensitivity is the use of a capture antibody that specifically binds to the target antigen, allowing for the detection of specific antibodies.
ELISA Process
- The maintenance of optimal pH and stability throughout the ELISA process is crucial, as it affects the accuracy and reliability of the results.
Indirect ELISA
- The two-step process in Indirect ELISA involves the initial binding of the antigen to the solid phase, followed by the addition of the primary antibody, allowing for the detection of specific antibodies.
ELISA Initial Step
- Coating the solid phase in the initial step of the ELISA process is crucial, as it allows for the immobilization of the antigen or antibody, enabling the detection of specific antibodies.
Explore the principles and variations of ELISA, a crucial immunological technique for detecting specific proteins, antibodies, or antigens in biological samples. Learn about the unique features of the Sandwich ELISA, including its 'sandwich' structure and enhanced specificity and sensitivity.
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