10 Questions
What is the primary purpose of using TE buffer to store DNA?
To chelate cations that can degrade DNA
Which of the following is the primary mechanism by which DNA is precipitated from a solution using ethanol?
Reduction in the dielectric constant of the solvent
What is the primary purpose of the washing step with 70% ethanol during DNA extraction?
To remove excess salts from the DNA
Which of the following properties of DNA allows it to be separated by size using gel electrophoresis?
The negative charge of the DNA backbone
What is the primary function of Tris in the TE buffer used to store DNA?
To maintain a stable pH for the DNA
Which of the following is the primary mechanism by which EDTA in TE buffer protects DNA from degradation?
By chelating divalent metal ions required for DNase activity
What is the primary function of the gel matrix in gel electrophoresis?
To create small pores that the DNA molecules must travel through
Which of the following is a key advantage of using magnetic beads for DNA extraction compared to traditional organic extraction methods?
Faster processing time
What is the primary mechanism by which DNA is separated from cellular components during organic extraction methods?
Differential precipitation of DNA and other cellular components
What is the primary advantage of using spectrophotometry to assess the quality and quantity of extracted DNA compared to gel electrophoresis?
Providing information on DNA purity and concentration
Learn about the salting-out method, a simple and nontoxic DNA extraction technique based on the principle that proteins and cellular contaminants precipitate in a saturated salt solution while DNA does not. Discover how to recover DNA using this method.
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