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Questions and Answers
What is the primary purpose of using TE buffer to store DNA?
What is the primary purpose of using TE buffer to store DNA?
- To provide a suitable pH for DNA storage
- To precipitate DNA from the solution
- To increase the solubility of DNA in water
- To chelate cations that can degrade DNA (correct)
Which of the following is the primary mechanism by which DNA is precipitated from a solution using ethanol?
Which of the following is the primary mechanism by which DNA is precipitated from a solution using ethanol?
- Dehydration of the DNA by the ethanol
- Reduction in the dielectric constant of the solvent (correct)
- Neutralization of the DNA's negative charges
- Formation of hydrogen bonds between DNA and ethanol
What is the primary purpose of the washing step with 70% ethanol during DNA extraction?
What is the primary purpose of the washing step with 70% ethanol during DNA extraction?
- To remove excess salts from the DNA (correct)
- To precipitate the DNA more efficiently
- To denature any contaminating proteins
- To increase the solubility of the DNA in water
Which of the following properties of DNA allows it to be separated by size using gel electrophoresis?
Which of the following properties of DNA allows it to be separated by size using gel electrophoresis?
What is the primary function of Tris in the TE buffer used to store DNA?
What is the primary function of Tris in the TE buffer used to store DNA?
Which of the following is the primary mechanism by which EDTA in TE buffer protects DNA from degradation?
Which of the following is the primary mechanism by which EDTA in TE buffer protects DNA from degradation?
What is the primary function of the gel matrix in gel electrophoresis?
What is the primary function of the gel matrix in gel electrophoresis?
Which of the following is a key advantage of using magnetic beads for DNA extraction compared to traditional organic extraction methods?
Which of the following is a key advantage of using magnetic beads for DNA extraction compared to traditional organic extraction methods?
What is the primary mechanism by which DNA is separated from cellular components during organic extraction methods?
What is the primary mechanism by which DNA is separated from cellular components during organic extraction methods?
What is the primary advantage of using spectrophotometry to assess the quality and quantity of extracted DNA compared to gel electrophoresis?
What is the primary advantage of using spectrophotometry to assess the quality and quantity of extracted DNA compared to gel electrophoresis?
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