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Salting-Out Method of DNA Extraction

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Questions and Answers

What is the primary purpose of using TE buffer to store DNA?

To provide a suitable pH for DNA storage

To precipitate DNA from the solution

To increase the solubility of DNA in water

To chelate cations that can degrade DNA (correct)

Which of the following is the primary mechanism by which DNA is precipitated from a solution using ethanol?

Dehydration of the DNA by the ethanol

Reduction in the dielectric constant of the solvent (correct)

Neutralization of the DNA's negative charges

Formation of hydrogen bonds between DNA and ethanol

What is the primary purpose of the washing step with 70% ethanol during DNA extraction?

To remove excess salts from the DNA (correct)

To precipitate the DNA more efficiently

To denature any contaminating proteins

To increase the solubility of the DNA in water

Which of the following properties of DNA allows it to be separated by size using gel electrophoresis?

The negative charge of the DNA backbone Signup and view all the answers

What is the primary function of Tris in the TE buffer used to store DNA?

To maintain a stable pH for the DNA Signup and view all the answers

Which of the following is the primary mechanism by which EDTA in TE buffer protects DNA from degradation?

By chelating divalent metal ions required for DNase activity Signup and view all the answers

What is the primary function of the gel matrix in gel electrophoresis?

To create small pores that the DNA molecules must travel through Signup and view all the answers

Which of the following is a key advantage of using magnetic beads for DNA extraction compared to traditional organic extraction methods?

Faster processing time Signup and view all the answers

What is the primary mechanism by which DNA is separated from cellular components during organic extraction methods?

Differential precipitation of DNA and other cellular components Signup and view all the answers

What is the primary advantage of using spectrophotometry to assess the quality and quantity of extracted DNA compared to gel electrophoresis?

Providing information on DNA purity and concentration Signup and view all the answers