RNA Transcription and Modification Quiz

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What is the function of the long C-terminal tail of RNA polymerase II in eukaryotic mRNA processing?

Cleaves the pre-mRNA to form mature mRNA.

Directly transfers capping and splicing components to the emerging RNA. (correct)

Coordinates with ribosomes for translation initiation.

Binds to the consensus sequence AAUAAA.

Which protein binds to the hexamer AAUAAA in eukaryotic mRNA processing?

RNA polymerase II

Poly-A polymerase

CPSF (correct)

CstF

In eukaryotic mRNA processing, what is the role of the CA sequence?

Promotes splicing of exons.

Facilitates transcription initiation.

Binds to the hexamer AAUAAA.

Required for the cleavage step. (correct)

What happens when a mutation leaves a normal splice site without a partner in eukaryotic mRNA processing?

An exon is skipped. (A) Signup and view all the answers

Which process ensures that the 3’ end of the pre-mRNA is appropriately processed by RNA polymerase II?

Polyadenylation (C) Signup and view all the answers

What happens when RNA polymerase II terminates transcription at the end of a gene?

It uses a mechanism to ensure proper processing of the 3’ end of the pre-mRNA. (D) Signup and view all the answers

What is the role of the RNA polymerase tail in RNA splicing?

It binds only a few critical components that act as a nucleation site for the remaining splicing components. (C) Signup and view all the answers

Which enzyme is responsible for adding a modified guanine nucleotide at the 5’ end of a new RNA molecule?

Guanyl transferase (C) Signup and view all the answers

In eukaryotic mRNA processing, what is the function of a methyl transferase enzyme?

Adds a methyl group to the guanosine. (D) Signup and view all the answers

What happens once RNA polymerase II has produced about 25 nucleotides of RNA?

The 5’ end of the new RNA molecule is modified by addition of a ‘cap’. (C) Signup and view all the answers

Which proteins seem to 'hop' from the polymerase tail onto the nascent RNA molecule for processing?

Splicing enzymes (D) Signup and view all the answers

What can RNA polymerase II in elongation mode be viewed as?

An RNA factory that transcribes DNA into RNA and processes the RNA it produces (B) Signup and view all the answers

What modifications are made to the ends of eukaryotic mRNA?

Capping on the 5' end and polyadenylation of the 3' end (D) Signup and view all the answers

What is responsible for removing intron sequences from eukaryotic RNA transcripts?

RNA splicing (B) Signup and view all the answers

What is added to the RNA before transcription is completed?

RNA cap (C) Signup and view all the answers

What type of sequences are present in eukaryotic mRNA resulting from transcription?

Coding (exon) and noncoding (intron) sequences (B) Signup and view all the answers

What is depicted as occurring concurrently in the RNA processing steps?

The addition of the RNA cap and RNA splicing (D) Signup and view all the answers

Which enzyme is responsible for adding the RNA cap to eukaryotic mRNA?

Poly-A polymerase (C) Signup and view all the answers