RNA Splicing in Gene Coding Sequences Quiz
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Questions and Answers

What term is used for the coding sequences in eukaryotic genes?

  • Promoters
  • Exons (correct)
  • Enhancers
  • Introns
  • What are the non-coding sequences that interrupt the coding sequence in eukaryotic genes called?

  • Silencers
  • Transposons
  • Exons
  • Introns (correct)
  • In eukaryotic genes, what must be done to the introns and exons post-transcription?

  • Introns and exons must remain as they are.
  • Exons must be removed, and introns must be joined together.
  • Introns must be translated into proteins.
  • Introns must be removed, and exons must be joined together. (correct)
  • Which term applies to any region retained in a mature RNA, whether or not it is coding?

    <p>Exon</p> Signup and view all the answers

    What are examples of non-coding exons mentioned in the text?

    <p>50 and 30 untranslated regions of an mRNA</p> Signup and view all the answers

    How many introns are shown to interrupt the coding region in a typical eukaryotic gene in Figure 14-1?

    <p>Three</p> Signup and view all the answers

    What is the process that converts pre-mRNA into mature mRNA by removing introns?

    <p>Splicing</p> Signup and view all the answers

    Why is precision in the splicing process crucial?

    <p>To prevent the loss or addition of nucleotides</p> Signup and view all the answers

    What consequence could occur if a base is lost or gained at the boundary between two exons during splicing?

    <p>Incorrect amino acids incorporated into proteins</p> Signup and view all the answers

    Which term describes the phenomenon where some pre-mRNAs can be spliced in more than one way?

    <p>Alternative splicing</p> Signup and view all the answers

    What would be the consequence of having downstream codons out of register due to lack of precision in splicing?

    <p>Incorrect amino acid incorporation</p> Signup and view all the answers

    Which process enables a gene to produce more than one polypeptide product?

    <p>Alternative splicing</p> Signup and view all the answers

    What are the products generated by alternative splicing called?

    <p>Isoforms</p> Signup and view all the answers

    How many alternative versions can the Slo gene from rat potentially encode?

    <p>500</p> Signup and view all the answers

    What process can result in one particular Drosophila gene encoding as many as 38,000 possible products?

    <p>Alternative splicing</p> Signup and view all the answers

    In what organism was splicing discovered during studies of gene expression?

    <p>Mammalian adenovirus</p> Signup and view all the answers

    What is another way in which initial transcripts can be altered apart from alternative splicing?

    <p>RNA editing</p> Signup and view all the answers

    What determines where splicing occurs within the RNA molecule?

    <p>RNA sequences</p> Signup and view all the answers

    What marks the borders between introns and exons in pre-mRNAs?

    <p>Specific nucleotide sequences</p> Signup and view all the answers

    Which sequence is necessary for splicing and is usually close to the 3' end of the intron?

    <p>Branchpoint sequence</p> Signup and view all the answers

    What is found at the 5' end of an intron?

    <p>5' splice site</p> Signup and view all the answers

    What is the conserved base at the branch site during splicing?

    <p>Adenine (A)</p> Signup and view all the answers

    Which sequence is sometimes referred to as the 3' site in older nomenclature?

    <p>3' splice site</p> Signup and view all the answers

    What is the function of the 5' and 3' splice sites?

    <p>Mark the exon – intron boundary for splicing</p> Signup and view all the answers

    What is responsible for generating separate mRNAs for individual virion components?

    <p>Alternative splicing</p> Signup and view all the answers

    Which non-protein-coding sequences are involved in stitching together the 50 sequence shared by all mRNAs?

    <p>Tripartite leader</p> Signup and view all the answers

    What experiment involves incubating RNA with double-stranded DNA to observe the formation of loops?

    <p>R-loop mapping</p> Signup and view all the answers

    What can be observed in the electron microscope following the staining procedure used to visualize nucleic acids?

    <p>Thicker RNA – DNA duplexes</p> Signup and view all the answers

    Which region of the genome do the late viral mRNAs arise from?

    <p>Multiple regions of the genome</p> Signup and view all the answers

    What is displaced in the form of a loop during an R-loop mapping experiment?

    <p>A stretch of the noncomplementary strand</p> Signup and view all the answers

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