RNA Splicing and Transcript Processing
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Questions and Answers

What is the structure that RNA polymerase recognizes to terminate transcription in prokaryotes?

  • Hairpin structure (correct)
  • 5' cap
  • Ribosome
  • Poly(A) signal
  • In eukaryotic transcription, what is transcribed downstream of the poly(A) signal?

  • mRNA (correct)
  • tRNA
  • snRNA
  • rRNA
  • Which component is responsible for adding the poly(A) tail in eukaryotic mRNA processing?

  • Poly(A) polymerase (correct)
  • DNA polymerase
  • RNA polymerase
  • Ribosome
  • What is the function of a 5' cap added to eukaryotic mRNA?

    <p>Enhances translation efficiency (D)</p> Signup and view all the answers

    What is the nucleotide added to the 3' end of a growing RNA polynucleotide during transcription?

    <p>Uracil (A)</p> Signup and view all the answers

    Which component is responsible for cutting introns and joining exons during RNA splicing?

    <p>Spliceosome (D)</p> Signup and view all the answers

    Which chemical modification process involves the excision of certain sequences known as introns?

    <p>RNA splicing (A)</p> Signup and view all the answers

    In eukaryotes, what process involves adding a long string of adenine nucleotides to the 3' end of the mRNA?

    <p>Polyadenylation (B)</p> Signup and view all the answers

    What is the name of the RNA molecule that combines with the ribosome to direct protein synthesis?

    <p>mRNA (D)</p> Signup and view all the answers

    Which of the following occurs before mRNA is translated by the ribosome in eukaryotes?

    <p>Addition of a 5' cap (C)</p> Signup and view all the answers

    What is the main function of a 5' cap added to mRNA in eukaryotes?

    <p>Prevent degradation (C)</p> Signup and view all the answers

    In prokaryotes, what is the relationship between the primary transcript and mRNA?

    <p>They are identical (C)</p> Signup and view all the answers

    What is the purpose of adding a 5' cap to a primary RNA transcript?

    <p>To enable ribosomes to bind and protect the mRNA from degradation (B)</p> Signup and view all the answers

    What is the function of a 3' poly(A) tail added to a primary RNA transcript?

    <p>To provide stability during translation and protect the mRNA from degradation (A)</p> Signup and view all the answers

    Which process allows a single gene to code for multiple proteins?

    <p>RNA splicing (A)</p> Signup and view all the answers

    What happens to introns during RNA processing in eukaryotes?

    <p>They are removed and remain inside the nucleus (B)</p> Signup and view all the answers

    What is the role of UTRs in mature mRNAs?

    <p>To protect the mRNA from degradation (D)</p> Signup and view all the answers

    Which term best describes alternative RNA splicing?

    <p>Production of different proteins from a single gene (A)</p> Signup and view all the answers

    Which statement accurately describes a characteristic of RNA processing in eukaryotes?

    <p>Exons are cut out before mRNA leaves the nucleus. (C)</p> Signup and view all the answers

    Why is it crucial to have a primary transcript that is longer than the final RNA molecule that leaves the nucleus?

    <p>To allow for alternative splicing and generating diversity in proteins. (D)</p> Signup and view all the answers

    What is the role of polyadenylation in RNA processing?

    <p>To add a poly-A tail to the 3' end of the RNA molecule. (C)</p> Signup and view all the answers

    Which aspect contributes to making an interaction between adenine and cytosine in DNA unstable?

    <p>Adenine forming only two hydrogen bonds instead of three. (C)</p> Signup and view all the answers

    What happens during 5' cap addition in mRNA processing?

    <p>Addition of methylated guanine nucleotide to the 5' end. (A)</p> Signup and view all the answers

    In RNA splicing, what is the function of ribozymes?

    <p>Catalyzing the removal of introns and joining of exons. (B)</p> Signup and view all the answers

    What is the purpose of the 5' cap addition in mRNA processing?

    <p>To protect the mRNA from degradation (C)</p> Signup and view all the answers

    How does the absence of the polyA tail affect mRNA translation?

    <p>It reduces mRNA stability (D)</p> Signup and view all the answers

    What role does RNA splicing play in mRNA processing?

    <p>Removing introns and joining exons (C)</p> Signup and view all the answers

    In eukaryotes, where does the process of transcription occur?

    <p>Nucleus (C)</p> Signup and view all the answers

    Which of the following best describes the function of tRNA in protein synthesis?

    <p>Carrying amino acids to the ribosome based on codon recognition (C)</p> Signup and view all the answers

    What impact would a mutation in the DNA sequence have on mRNA translation?

    <p>It might change the amino acid sequence in the resulting protein (B)</p> Signup and view all the answers

    Where does the small ribosomal subunit bind during translation initiation in bacteria?

    <p>Ribosome binding site (D)</p> Signup and view all the answers

    In translation, what is the role of the initiator tRNA?

    <p>Carries a modified methionine (A)</p> Signup and view all the answers

    What happens at the termination phase of translation?

    <p>Release factor binds to the stop codon (A)</p> Signup and view all the answers

    Where are amino acids added during translation?

    <p>A site (B)</p> Signup and view all the answers

    During translation elongation, what happens when the ribosome moves down the mRNA by one codon?

    <p>P site tRNA exits (D)</p> Signup and view all the answers

    What defines where the next tRNA will bind during protein synthesis?

    <p>Codon-anticodon recognition (A)</p> Signup and view all the answers

    What is the main role of RNA splicing in mRNA processing?

    <p>Joining exons and excising introns (B)</p> Signup and view all the answers

    Which component plays a vital role in protecting mRNA from degradation?

    <p>Poly(A) tail (B)</p> Signup and view all the answers

    What is the function of the 5' cap added to eukaryotic mRNA?

    <p>Initiating translation by attracting ribosomes (A)</p> Signup and view all the answers

    Which process leads to the removal of introns during mRNA processing?

    <p>RNA splicing (B)</p> Signup and view all the answers

    What occurs during translocation in translation?

    <p>The tRNA in the P site moves into the E site and exits the ribosome. (C)</p> Signup and view all the answers

    What is the primary purpose of adding a poly(A) tail to mRNA?

    <p>Stabilizing mRNA against degradation (D)</p> Signup and view all the answers

    What happens to the tRNA attached to the growing protein during translation termination?

    <p>It moves to the E site for ejection. (C)</p> Signup and view all the answers

    What is the role of a release factor in translation termination?

    <p>Hydrolyzes the bond linking the A-site tRNA to the polypeptide chain. (B)</p> Signup and view all the answers

    Which region of mature mRNAs plays a crucial role in regulating translation efficiency?

    <p>UTRs (A)</p> Signup and view all the answers

    What is the purpose of post-translational modifications in protein processing?

    <p>Determine protein shape and function. (A)</p> Signup and view all the answers

    Which step follows translation in protein synthesis?

    <p>Post-Translational Modifications (C)</p> Signup and view all the answers

    What determines a protein's shape and function before it becomes functional?

    <p>Post-translational modifications (D)</p> Signup and view all the answers

    During transcription, what do promoters indicate and where are they located?

    <p>The start of a gene and the template DNA strand; downstream of the transcription start site (B)</p> Signup and view all the answers

    What happens when RNA polymerase binds to the DNA duplex during initiation in transcription?

    <p>It opens the DNA double helix and creates a transcription bubble. (B)</p> Signup and view all the answers

    Which direction does the new RNA strand grow during transcription?

    <p>5' to 3' (C)</p> Signup and view all the answers

    What is the function of nucleoside triphosphates (NTPs) in RNA synthesis?

    <p>They are used to produce an RNA transcript complementary to the template DNA strand. (B)</p> Signup and view all the answers

    What role do tRNAs play in the process of translation?

    <p>They carry amino acids to the ribosome for protein synthesis. (C)</p> Signup and view all the answers

    Which cellular component is primarily responsible for post-translational modifications of proteins?

    <p>Golgi apparatus (C)</p> Signup and view all the answers

    What is the primary function of RNA splicing in mRNA processing?

    <p>Excising introns from the primary transcript (A)</p> Signup and view all the answers

    How is the primary transcript different from mRNA in eukaryotes?

    <p>Primary transcript undergoes RNA splicing (C)</p> Signup and view all the answers

    Which modification occurs at the 3' end of the mRNA during RNA processing?

    <p>Polyadenylation (B)</p> Signup and view all the answers

    What is the significance of the 5' cap addition in mRNA processing?

    <p>Enhancing translation efficiency (C)</p> Signup and view all the answers

    During transcription, the template strand is used to synthesize an RNA transcript that is complementary to the non-template strand of DNA.

    <p>False (B)</p> Signup and view all the answers

    Nucleoside triphosphates (NTPs) pair with complementary RNA bases during transcription.

    <p>False (B)</p> Signup and view all the answers

    RNA polymerase binds to the DNA duplex at terminator sequences during transcription initiation.

    <p>False (B)</p> Signup and view all the answers

    The new RNA strand grows in the 3' to 5' direction during transcription.

    <p>False (B)</p> Signup and view all the answers

    Promoters in transcription indicate where a gene ends and which DNA strand is the template strand.

    <p>False (B)</p> Signup and view all the answers

    During transcription, RNA polymerase creates a transcription bubble by separating the two strands of DNA.

    <p>True (A)</p> Signup and view all the answers

    RNA polymerase transcribes a transcription-initiation signal.

    <p>False (B)</p> Signup and view all the answers

    In prokaryotes, a hairpin structure is formed by the RNA downstream of the termination signal.

    <p>False (B)</p> Signup and view all the answers

    Eukaryotic transcription uses a hairpin structure as a termination signal.

    <p>False (B)</p> Signup and view all the answers

    DNA polymerase is directly involved in transcription in both prokaryotes and eukaryotes.

    <p>False (B)</p> Signup and view all the answers

    In prokaryotic transcription, ribosomes are required for the process.

    <p>False (B)</p> Signup and view all the answers

    RNA nucleotides can be added to either end of a growing RNA polynucleotide during transcription.

    <p>False (B)</p> Signup and view all the answers

    In prokaryotes, the primary transcript is synonymous with mRNA.

    <p>True (A)</p> Signup and view all the answers

    RNA splicing involves the excision of exons, leaving only the introns intact.

    <p>False (B)</p> Signup and view all the answers

    Polyadenylation is a type of chemical modification that occurs before mRNA translation by the ribosome.

    <p>True (A)</p> Signup and view all the answers

    RNA splicing occurs in both prokaryotes and eukaryotes at the same efficiency rate.

    <p>False (B)</p> Signup and view all the answers

    The primary function of a 5' cap added to mRNA is to indicate the start codon for translation.

    <p>False (B)</p> Signup and view all the answers

    In eukaryotes, the primary transcript contains only the gene information transcribed, while mRNA includes additional regulatory elements.

    <p>True (A)</p> Signup and view all the answers

    DNA and RNA have the same nucleotide bases.

    <p>False (B)</p> Signup and view all the answers

    In RNA molecules, uracil pairs with thymine.

    <p>False (B)</p> Signup and view all the answers

    During transcription, the DNA double helix separates to allow the synthesis of RNA.

    <p>True (A)</p> Signup and view all the answers

    RNA polymerase adds nucleotides to the 5' end of the growing RNA strand during transcription.

    <p>False (B)</p> Signup and view all the answers

    The mRNA produced during transcription is ready for translation without any further processing.

    <p>False (B)</p> Signup and view all the answers

    Transcription occurs in the cytoplasm of eukaryotic cells.

    <p>False (B)</p> Signup and view all the answers

    During translation elongation, the uncharged tRNA from the A site moves into the E site and is ejected from the ribosome.

    <p>True (A)</p> Signup and view all the answers

    In translation, the initiator tRNA is in the P site at the start of elongation.

    <p>True (A)</p> Signup and view all the answers

    Translocation during elongation occurs when the ribosome moves one codon towards the 5' end of the mRNA.

    <p>False (B)</p> Signup and view all the answers

    During translation termination, a release factor enters the P site of the ribosome.

    <p>False (B)</p> Signup and view all the answers

    Aminoacyl tRNA binds to the codon in the E site during translation elongation.

    <p>False (B)</p> Signup and view all the answers

    In translation termination, a release factor resembles tRNAs in size and shape.

    <p>True (A)</p> Signup and view all the answers

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