18 Questions
What is the flow of Ty-1 sequence information during transposition?
DNA to RNA to DNA
What can transposable elements cause in the genome?
Additional rearrangements
What is the outcome of recombination between two transposable elements in the same orientation?
Deletion
What is the predicted mechanism if yeast Ty-1 moved as illustrated for DNA-mediated replicative transposition?
RNA-mediated transposition
What happens if recombination occurs between two transposable elements in opposite orientations?
Inversion
Does the retrotransposition machinery have an absolute preference for LINE1 mRNA?
No, the preference is not absolute
What is the primary defining feature of transposons?
They can move from one genetic location to another.
Which type of cells contain transposons?
Both prokaryotic and eukaryotic cells
What is one role of transposases in the transposition process?
Catalyzing the movement of transposons
How do transposons cause genetic mutations?
By adding new genetic material
Which statement accurately describes transposons in the human genome?
They constitute approximately 50 to 65% of the genome.
What distinguishes the two major classes of transposable elements?
The intermediates involved in the transposition process
What is the main difference between replicative and nonreplicative transposition?
DNA synthesis occurs at the crossover structure in replicative transposition but not in nonreplicative transposition.
How does replicative transposition differ from nonreplicative transposition in terms of DNA synthesis?
DNA synthesis results in a copy at both donor and target sites in replicative transposition.
What is the significance of the strand-transfer intermediate in transposition?
It attaches the transposable element to both donor and recipient replicons.
How does nonreplicative transposition differ from replicative transposition in terms of cointegrate intermediate formation?
Cointegrate intermediate is produced through DNA synthesis in replicative transposition.
What happens to the transposon in an alternative pathway for nonreplicative transposition?
It is joined to the recipient at a staggered break.
What is common between replicative and nonreplicative transposition regarding generating the strand-transfer intermediate?
DNA synthesis occurs at this stage for both types of transposition.
Test your understanding of RNA-mediated transposition with this exercise. Predict the outcomes of a transposition experiment involving yeast Ty-1 moving by a replicative transposition mechanism. Consider the effects of inducing transcription on transposition rates.
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