RNA Mediated Regulation

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What is the primary function of Micro RNA (miRNA)?

To synthesise protein molecules

To regulate endogenous gene expression (correct)

To regulate exogenous gene expression

To activate RNAi silencing pathway

What is the role of Drosha in miRNA biogenesis?

To export pre-miRNA to the cytoplasm

To cleave pri-miRNA to produce pre-miRNA (correct)

To cleave the terminal loop of pre-miRNA

To bind to the double-stranded RNA binding protein DGCR8

What is the function of Exportin 5 in miRNA biogenesis?

To bind to pre-miRNA and transport it to the cytoplasm (correct)

To cleave pri-miRNA to produce pre-miRNA

To bind to the double-stranded RNA binding protein DGCR8

To select the guide strand of miRNA

What is the role of Dicer in miRNA biogenesis?

To cleave the terminal loop of pre-miRNA to create miRNA:miRNA duplex (D) Signup and view all the answers

What is the function of the RISC loading complex?

To load the guide strand of miRNA into the RISC (C) Signup and view all the answers

What is the mechanism of miRNA-mediated gene regulation?

Deadenylation followed by degradation (C) Signup and view all the answers

What is the characteristic of RNAase III enzymes?

Specificity for double-stranded RNA (B) Signup and view all the answers

What is the purpose of miRNA sequencing?

To identify novel miRNA molecules (A) Signup and view all the answers

What is the consequence of a mutation in miR-96?

Hereditary progressive hearing loss (C) Signup and view all the answers

What is the potential application of RNAi in therapy?

To silence detrimental genes and viruses (C) Signup and view all the answers

What is the primary site of pre-miRNA processing?

Nucleus (C) Signup and view all the answers

Which complex is responsible for the cleavage of pri-miRNA?

Microprocessor complex (A) Signup and view all the answers

What is the purpose of the double-stranded RNA-binding domain (dsRBD) in RNAase III enzymes?

To recognize and bind double-stranded RNA (C) Signup and view all the answers

What is the consequence of a mutation in miR-184?

Hereditary keratoconus and cataracts (D) Signup and view all the answers

What is the function of TRBP in miRNA biogenesis?

To bind and stabilize Dicer (C) Signup and view all the answers

What is the purpose of miRNA sequencing?

To study miRNA expression profiles (D) Signup and view all the answers

Which of the following is NOT a characteristic of RNAase III enzymes?

Cleave single-stranded RNA (A) Signup and view all the answers

What is the purpose of the RISC loading complex?

To load miRNA into the RISC complex (A) Signup and view all the answers

What is the consequence of down-regulating VEGF using siRNA?

Decreased angiogenesis (B) Signup and view all the answers

What is the function of Exportin 5 in miRNA biogenesis?

To export pre-miRNA from the nucleus (D) Signup and view all the answers

Which of the following is a characteristic of Class I RNAase III enzymes?

Contains 1 RNAase III domain and 1 dsRBD (A) Signup and view all the answers

What is the purpose of the microprocessor complex in miRNA biogenesis?

To cleave the pri-miRNA into a pre-miRNA hairpin (D) Signup and view all the answers

What is the function of the passenger strand in the RISC loading complex?

To be degraded and discarded (D) Signup and view all the answers

What is the consequence of a mutation in the DICER gene?

Abnormal miRNA processing and regulation (A) Signup and view all the answers

What is the purpose of miRNA arrays?

To study miRNA expression profiles (A) Signup and view all the answers

How does miRNA-mediated gene regulation occur?

By binding to the 3' end of the target mRNA (D) Signup and view all the answers

What is the function of the double-stranded RNA-binding domain (dsRBD) in RNAase III enzymes?

To recognize and bind to double-stranded RNA (C) Signup and view all the answers

What is the consequence of injecting siRNA that down-regulates VEGF into the eye?

Decreased VEGF expression (C) Signup and view all the answers

What is the function of TRBP in miRNA biogenesis?

To interact with Dicer and facilitate pre-miRNA processing (D) Signup and view all the answers

What is the characteristic of the RISC loading complex?

It is a complex that loads the guide strand into the RISC complex (B) Signup and view all the answers

RNA Mediated Regulation

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Questions and Answers

What is the primary function of Micro RNA (miRNA)?

What is the role of Drosha in miRNA biogenesis?

What is the function of Exportin 5 in miRNA biogenesis?

What is the role of Dicer in miRNA biogenesis?

What is the function of the RISC loading complex?

What is the mechanism of miRNA-mediated gene regulation?

What is the characteristic of RNAase III enzymes?

What is the purpose of miRNA sequencing?

What is the consequence of a mutation in miR-96?

What is the potential application of RNAi in therapy?

What is the primary site of pre-miRNA processing?

Which complex is responsible for the cleavage of pri-miRNA?

What is the purpose of the double-stranded RNA-binding domain (dsRBD) in RNAase III enzymes?

What is the consequence of a mutation in miR-184?

What is the function of TRBP in miRNA biogenesis?

What is the purpose of miRNA sequencing?

Which of the following is NOT a characteristic of RNAase III enzymes?

What is the purpose of the RISC loading complex?

What is the consequence of down-regulating VEGF using siRNA?

What is the function of Exportin 5 in miRNA biogenesis?

Which of the following is a characteristic of Class I RNAase III enzymes?

What is the purpose of the microprocessor complex in miRNA biogenesis?

What is the function of the passenger strand in the RISC loading complex?

What is the consequence of a mutation in the DICER gene?

What is the purpose of miRNA arrays?

How does miRNA-mediated gene regulation occur?

What is the function of the double-stranded RNA-binding domain (dsRBD) in RNAase III enzymes?

What is the consequence of injecting siRNA that down-regulates VEGF into the eye?

What is the function of TRBP in miRNA biogenesis?

What is the characteristic of the RISC loading complex?

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