30 Questions
What is the primary function of Micro RNA (miRNA)?
To regulate endogenous gene expression
What is the role of Drosha in miRNA biogenesis?
To cleave pri-miRNA to produce pre-miRNA
What is the function of Exportin 5 in miRNA biogenesis?
To bind to pre-miRNA and transport it to the cytoplasm
What is the role of Dicer in miRNA biogenesis?
To cleave the terminal loop of pre-miRNA to create miRNA:miRNA duplex
What is the function of the RISC loading complex?
To load the guide strand of miRNA into the RISC
What is the mechanism of miRNA-mediated gene regulation?
Deadenylation followed by degradation
What is the characteristic of RNAase III enzymes?
Specificity for double-stranded RNA
What is the purpose of miRNA sequencing?
To identify novel miRNA molecules
What is the consequence of a mutation in miR-96?
Hereditary progressive hearing loss
What is the potential application of RNAi in therapy?
To silence detrimental genes and viruses
What is the primary site of pre-miRNA processing?
Nucleus
Which complex is responsible for the cleavage of pri-miRNA?
Microprocessor complex
What is the purpose of the double-stranded RNA-binding domain (dsRBD) in RNAase III enzymes?
To recognize and bind double-stranded RNA
What is the consequence of a mutation in miR-184?
Hereditary keratoconus and cataracts
What is the function of TRBP in miRNA biogenesis?
To bind and stabilize Dicer
What is the purpose of miRNA sequencing?
To study miRNA expression profiles
Which of the following is NOT a characteristic of RNAase III enzymes?
Cleave single-stranded RNA
What is the purpose of the RISC loading complex?
To load miRNA into the RISC complex
What is the consequence of down-regulating VEGF using siRNA?
Decreased angiogenesis
What is the function of Exportin 5 in miRNA biogenesis?
To export pre-miRNA from the nucleus
Which of the following is a characteristic of Class I RNAase III enzymes?
Contains 1 RNAase III domain and 1 dsRBD
What is the purpose of the microprocessor complex in miRNA biogenesis?
To cleave the pri-miRNA into a pre-miRNA hairpin
What is the function of the passenger strand in the RISC loading complex?
To be degraded and discarded
What is the consequence of a mutation in the DICER gene?
Abnormal miRNA processing and regulation
What is the purpose of miRNA arrays?
To study miRNA expression profiles
How does miRNA-mediated gene regulation occur?
By binding to the 3' end of the target mRNA
What is the function of the double-stranded RNA-binding domain (dsRBD) in RNAase III enzymes?
To recognize and bind to double-stranded RNA
What is the consequence of injecting siRNA that down-regulates VEGF into the eye?
Decreased VEGF expression
What is the function of TRBP in miRNA biogenesis?
To interact with Dicer and facilitate pre-miRNA processing
What is the characteristic of the RISC loading complex?
It is a complex that loads the guide strand into the RISC complex
Learn about the biogenesis and regulation of microRNA, a non-coding RNA molecule that plays a crucial role in regulating endogenous gene expression. Understand the pathway of miRNA biogenesis, including the formation of pri-miRNA, pre-miRNA, and its transport to the cytoplasm. Test your knowledge of RNA mediated regulation!
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