15 Questions
In the Cre-lox technique, what is the function of Cre-recombinase?
It binds to the loxP sequences flanking exon 2 and removes that exon
What paracrine factors does the optic vesicle secrete to initiate lens formation?
BMP and FGF
What is the promoter that activates the gene for Cre-recombinase in the Cre-lox technique?
Albumin gene promoter
What happens when Pax6 is lost in rats according to the text?
Failure to form eyes and reduced nasal structures
How does paracrine signaling differ from juxtracrine signaling?
Paracrine signaling occurs across multiple cell distances, while juxtracrine signaling occurs locally between neighboring cells
In which cells does Pax6 play a role for proper lens induction according to the text?
Surface ectoderm
What is the purpose of flanking the second exon with loxP sequences in the Cre-lox technique?
To allow for conditional mutagenesis in specific cells
What is a characteristic phenotype resulting from mutations in the Pax6 gene, as mentioned in the text?
Reduction in the iris of the eye
What do cells exposed to intermediate concentrations of morphogen do?
Activate a different set of genes and inhibit those induced at higher concentrations
What happens when Cre-recombinase binds to the loxP sequences flanking exon 2 in the Cre-lox technique?
The second exon is removed, causing gene mutations in specific cells
Where is ribosomal protein Rpl38 concentrated in mice?
In ribosomes found in somites
What is the consequence of Rpl38 deficiency in mice?
Reduced Hox gene translation and an extra pair of vertebrae
What happens to futile cycle mutant embryos?
They consistently show only 2 labeled nuclei
Where are miRNAs processed by the Drosha RNAase in the cell?
In the nucleus
What primarily makes up the RNA-induced silencing complex (RISC)?
Dicer and Argonaute
Explore the model of ribosomal heterogeneity in mice, focusing on the variation in ribosomal proteins and its impact on tissue-specific translation. Learn about the role of ribosomal protein Rpl38 in somites and its influence on vertebrae development and Hox gene translation.
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