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Reverse Vaccinology: Identifying Vaccine Candidates

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Reverse Vaccinology: Identifying Vaccine Candidates

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Questions and Answers

What is the purpose of taking a small piece of DNA from a virus or bacterium?

To study the pathogen's genetic sequence

To create a toxin that fights the pathogen

To produce a protein antigen for vaccination (correct)

To create a clone of the pathogen

What type of cells are commonly used as heterologous hosts for expressing immunogenic proteins?

Yeast cells, bacteria, insect cells, plant cells, and mammalian cell lines (correct)

Plant cells and mammalian cell lines

Fungal cells and mammalian cells

Bacterial cells and insect cells

What is the term for the part of an antigen molecule that an antibody attaches to?

Vaccine

Epitope (correct)

Antigen

Immunogen

How does the yeast cell contribute to the production of the Hepatitis B surface antigen (HBsAg)?

It performs post-translational modifications, including glycosylation (B) Signup and view all the answers

What is the form in which the HBsAg assembles after expression in yeast cells?

Virus-like particles (VLPs) (D) Signup and view all the answers

What is the advantage of producing HBsAg in yeast cells?

All of the above (D) Signup and view all the answers

What is the term for the process of using a part of the pathogen's DNA to produce a vaccine?

Recombinant protein vaccine production (D) Signup and view all the answers

What is the purpose of transforming the recombinant plasmids into yeast cells?

To produce the desired antigen (B) Signup and view all the answers

What is the benefit of using yeast cells for the production of HBsAg?

They allow for high expression levels and easy purification (A) Signup and view all the answers

What is the term for the study of the epitopes of a pathogen to identify potential vaccine targets?

Reverse vaccinology (D) Signup and view all the answers