Podcast
Questions and Answers
What is the main reason that restriction enzymes may exhibit 'star activity' and cut at sequences other than their expected recognition sequence?
What is the main reason that restriction enzymes may exhibit 'star activity' and cut at sequences other than their expected recognition sequence?
- The enzymes are inherently less specific under non-standard conditions. (correct)
- The enzymes have a divalent cation imbalance.
- The enzymes are exposed to prolonged reaction times.
- The enzymes are contaminated with organic solvents.
What is the significance of Restriction Fragment Length Polymorphisms (RFLPs)?
What is the significance of Restriction Fragment Length Polymorphisms (RFLPs)?
- RFLPs are used to detect structural changes in chromosomes associated with disease.
- RFLPs are the basis for the first molecular-based human identification and mapping methods.
- RFLPs are used to analyze the size and number of restriction fragments in an individual's DNA.
- All of the above. (correct)
Which of the following is NOT a condition that can induce 'star activity' in restriction enzymes?
Which of the following is NOT a condition that can induce 'star activity' in restriction enzymes?
- Suboptimal buffer conditions.
- Prolonged incubation at low temperature. (correct)
- pH greater than 8.0.
- High concentration of enzymes.
What is the main purpose of procedures performed in the clinical molecular laboratory?
What is the main purpose of procedures performed in the clinical molecular laboratory?
What is the significance of the Southern blot technique?
What is the significance of the Southern blot technique?
Which of the following can contribute to differences in the number and location of restriction sites for a given restriction enzyme in human DNA?
Which of the following can contribute to differences in the number and location of restriction sites for a given restriction enzyme in human DNA?
What is the primary purpose of the depurination step when preparing larger DNA fragments for blotting?
What is the primary purpose of the depurination step when preparing larger DNA fragments for blotting?
What is the primary indication that the restriction enzyme digest was incomplete based on the information provided?
What is the primary indication that the restriction enzyme digest was incomplete based on the information provided?
What is the recommended voltage and run time for resolving 10,000- to 20,000-bp DNA fragments in a 0.7% agarose gel?
What is the recommended voltage and run time for resolving 10,000- to 20,000-bp DNA fragments in a 0.7% agarose gel?
What is the purpose of staining the agarose gel with ethidium bromide and illuminating it with UV light?
What is the purpose of staining the agarose gel with ethidium bromide and illuminating it with UV light?
What is the primary indication that the DNA sample is degraded based on the information provided?
What is the primary indication that the DNA sample is degraded based on the information provided?
What is the purpose of the blotting (transfer) step after the DNA fragments have been resolved by electrophoresis?
What is the purpose of the blotting (transfer) step after the DNA fragments have been resolved by electrophoresis?
What does sequence complexity refer to?
What does sequence complexity refer to?
What does Cot 1/2 represent in nucleic acid sequences?
What does Cot 1/2 represent in nucleic acid sequences?
How do longer probes differ from shorter probes in terms of specificity?
How do longer probes differ from shorter probes in terms of specificity?
Why are short probes ideal for mutation analysis?
Why are short probes ideal for mutation analysis?
What is the effect of increased probe concentration on hybridization sensitivity?
What is the effect of increased probe concentration on hybridization sensitivity?
Where is hybridization generally performed?
Where is hybridization generally performed?
What is the recommended volume of hybridization buffer for 100 cm2 of membrane surface area?
What is the recommended volume of hybridization buffer for 100 cm2 of membrane surface area?
What is the primary function of formamide in the hybridization buffer?
What is the primary function of formamide in the hybridization buffer?
Which method is recommended for maintaining a constant temperature during hybridization?
Which method is recommended for maintaining a constant temperature during hybridization?
What is the optimal hybridization time for short probes (less than 20 bases)?
What is the optimal hybridization time for short probes (less than 20 bases)?
Which polymers can accelerate the hybridization rates for probes longer than 250 bases?
Which polymers can accelerate the hybridization rates for probes longer than 250 bases?
What is the optimal hybridization temperature for a double-stranded DNA probe?
What is the optimal hybridization temperature for a double-stranded DNA probe?
Which of the following is a key characteristic of bead array systems used for clinical tests?
Which of the following is a key characteristic of bead array systems used for clinical tests?
What is the main advantage of solution hybridization compared to other hybridization techniques?
What is the main advantage of solution hybridization compared to other hybridization techniques?
What key technological advancement allowed microarrays to become more versatile compared to macroarrays?
What key technological advancement allowed microarrays to become more versatile compared to macroarrays?
Which of the following is a common application of bead array systems in clinical testing?
Which of the following is a common application of bead array systems in clinical testing?
What is the key difference between solution hybridization and microarray techniques in terms of target and probe immobilization?
What is the key difference between solution hybridization and microarray techniques in terms of target and probe immobilization?
What is the main advantage of microarrays compared to macroarrays in terms of the number of targets that can be screened simultaneously?
What is the main advantage of microarrays compared to macroarrays in terms of the number of targets that can be screened simultaneously?
