12 Questions
What is a key factor in almost all transformation efforts according to the text?
Selection
Which technique involves using Agrobacterium tumefaciens for gene transfer?
Microinjection
What is a method used for transforming chloroplasts?
Protoplast
Which of the following is not a method for gene transfer mentioned in the text?
Microfiber 'whiskers'
What should be prepared along with gene constructs for successful transformation?
Suitable vectors
Which step involves the introduction of genes into crop plants?
Efficient transformation techniques
After transformation, what is done to ensure stable and efficient gene expression?
Molecular and genetic characterization of transgenic plants
Which method is used for transferring genes to elite cultivars if necessary?
"Transfer of genes to elite cultivars by conventional breeding methods"
Match the following with the step in transformation efforts: Preparation of gene constructs and transformation with suitable vectors
Electroporation = Efficient transformation techniques for the introduction of genes into crop plants Microinjection = Recovery and multiplication of transgenic plants Microfiber “whiskers” = Preparation of gene constructs and transformation with suitable vectors Laser mediated Biolistic - gene gun = Molecular and genetic characterization of transgenic plants for stable and efficient gene expression
Match the following with the method of gene transfer: Virus vectors
Liposome fusion = Virus vectors Agrobacterium tumefaciens = Protoplast PEG = Meristem transformation Protoplast = Virus vectors
Match the following with the technique for gene transfer: Embryogenic calli
Chloroplast = Embryogenic calli Meristem transformation = Pollen transformation Pollen transformation = Seed infection with Agrobacterium Seed infection with Agrobacterium = Embryogenic calli
Match the following with the method used for transformation: Biolistic - gene gun
Electroporation = Microinjection Microfiber “whiskers” = Laser mediated Biolistic - gene gun Virus vectors = Liposome fusion Agrobacterium tumefaciens = Biolistic - gene gun
Learn about the importance of selectable marker genes in regenerating transgenic plants and the application of selection pressure throughout different stages of plant development. Discover how selection is used for genetic analysis of inserted DNA during seed germination and seedling growth.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
