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Regeneration of Transgenic Plants: Selectable Marker Genes

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Regeneration of Transgenic Plants: Selectable Marker Genes

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Questions and Answers

What is a key factor in almost all transformation efforts according to the text?

Recovery and multiplication of transgenic plants

Molecular and genetic characterization of transgenic plants

Evaluation of transgenic plants for their effectiveness in alleviating stresses

Selection (correct)

Which technique involves using Agrobacterium tumefaciens for gene transfer?

Microinjection (correct)

Electroporation

Laser mediated Biolistic - gene gun

PEG

What is a method used for transforming chloroplasts?

Pollution transformation

Protoplast (correct)

Meristem transformation

Embryogenic calli

Which of the following is not a method for gene transfer mentioned in the text?

Microfiber 'whiskers' Signup and view all the answers

What should be prepared along with gene constructs for successful transformation?

Suitable vectors Signup and view all the answers

Which step involves the introduction of genes into crop plants?

Efficient transformation techniques Signup and view all the answers

After transformation, what is done to ensure stable and efficient gene expression?

Molecular and genetic characterization of transgenic plants Signup and view all the answers

Which method is used for transferring genes to elite cultivars if necessary?

"Transfer of genes to elite cultivars by conventional breeding methods" Signup and view all the answers

Match the following with the step in transformation efforts: Preparation of gene constructs and transformation with suitable vectors

Electroporation = Efficient transformation techniques for the introduction of genes into crop plants Microinjection = Recovery and multiplication of transgenic plants Microfiber “whiskers” = Preparation of gene constructs and transformation with suitable vectors Laser mediated Biolistic - gene gun = Molecular and genetic characterization of transgenic plants for stable and efficient gene expression Signup and view all the answers

Match the following with the method of gene transfer: Virus vectors

Liposome fusion = Virus vectors Agrobacterium tumefaciens = Protoplast PEG = Meristem transformation Protoplast = Virus vectors Signup and view all the answers

Match the following with the technique for gene transfer: Embryogenic calli

Chloroplast = Embryogenic calli Meristem transformation = Pollen transformation Pollen transformation = Seed infection with Agrobacterium Seed infection with Agrobacterium = Embryogenic calli Signup and view all the answers

Match the following with the method used for transformation: Biolistic - gene gun

Electroporation = Microinjection Microfiber “whiskers” = Laser mediated Biolistic - gene gun Virus vectors = Liposome fusion Agrobacterium tumefaciens = Biolistic - gene gun Signup and view all the answers