Regeneration of Transgenic Plants: Selectable Marker Genes
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Questions and Answers

What is a key factor in almost all transformation efforts according to the text?

  • Recovery and multiplication of transgenic plants
  • Molecular and genetic characterization of transgenic plants
  • Evaluation of transgenic plants for their effectiveness in alleviating stresses
  • Selection (correct)

Which technique involves using Agrobacterium tumefaciens for gene transfer?

  • Microinjection (correct)
  • Electroporation
  • Laser mediated Biolistic - gene gun
  • PEG

What is a method used for transforming chloroplasts?

  • Pollution transformation
  • Protoplast (correct)
  • Meristem transformation
  • Embryogenic calli

Which of the following is not a method for gene transfer mentioned in the text?

<p>Microfiber 'whiskers' (D)</p> Signup and view all the answers

What should be prepared along with gene constructs for successful transformation?

<p>Suitable vectors (B)</p> Signup and view all the answers

Which step involves the introduction of genes into crop plants?

<p>Efficient transformation techniques (D)</p> Signup and view all the answers

After transformation, what is done to ensure stable and efficient gene expression?

<p>Molecular and genetic characterization of transgenic plants (C)</p> Signup and view all the answers

Which method is used for transferring genes to elite cultivars if necessary?

<p>&quot;Transfer of genes to elite cultivars by conventional breeding methods&quot; (C)</p> Signup and view all the answers

Match the following with the step in transformation efforts: Preparation of gene constructs and transformation with suitable vectors

<p>Electroporation = Efficient transformation techniques for the introduction of genes into crop plants Microinjection = Recovery and multiplication of transgenic plants Microfiber “whiskers” = Preparation of gene constructs and transformation with suitable vectors Laser mediated Biolistic - gene gun = Molecular and genetic characterization of transgenic plants for stable and efficient gene expression</p> Signup and view all the answers

Match the following with the method of gene transfer: Virus vectors

<p>Liposome fusion = Virus vectors Agrobacterium tumefaciens = Protoplast PEG = Meristem transformation Protoplast = Virus vectors</p> Signup and view all the answers

Match the following with the technique for gene transfer: Embryogenic calli

<p>Chloroplast = Embryogenic calli Meristem transformation = Pollen transformation Pollen transformation = Seed infection with Agrobacterium Seed infection with Agrobacterium = Embryogenic calli</p> Signup and view all the answers

Match the following with the method used for transformation: Biolistic - gene gun

<p>Electroporation = Microinjection Microfiber “whiskers” = Laser mediated Biolistic - gene gun Virus vectors = Liposome fusion Agrobacterium tumefaciens = Biolistic - gene gun</p> Signup and view all the answers

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