Recombinant DNA Technology Overview
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Questions and Answers

Which of the following chemicals would be involved in a step that comes before step 1 in the recombinant DNA technology process?

  • all - lysozyme, ethanol, ribonuclease, cellulase, protease (correct)
  • only lysozyme and ethanol
  • only cellulase, lysozyme and ethanol
  • only lysozyme, ribonuclease, protease, and ethanol
  • The gene ______ codes for the enzyme β-galactosidase, which is responsible for breaking down lactose.

    LacZ

    Cells containing recombinant DNA will produce β-galactosidase and appear blue when plated on an appropriate medium.

    False (B)

    What is the purpose of DNA ligase in the recombinant DNA technology process?

    <p>DNA ligase is used to join the foreign DNA fragment with the vector DNA, creating a recombinant molecule.</p> Signup and view all the answers

    Match these steps in the recombinant DNA technology process to their corresponding descriptions:

    <p>Step 1 = The recombinant DNA is inserted into a host cell. Step 2 = The desired gene is isolated from a donor organism. Step 3 = The foreign DNA is inserted into the vector DNA. Step 4 = The host cell is used to replicate the recombinant DNA.</p> Signup and view all the answers

    Which of the following natural processes does polymerase chain reaction (PCR) most closely mimic?

    <p>replication (B)</p> Signup and view all the answers

    Steam in a bio-processor is utilized to increase the rate of growth for organisms.

    <p>False (B)</p> Signup and view all the answers

    Recombinant DNA technology, similar to sexual reproduction, contributes to which of the following?

    <p>only genetic mutation and genetic variation (B)</p> Signup and view all the answers

    What is the name of the bird that was at risk of extinction due to the use of DDT, as mentioned in the research paper 'Ecological Risk of DDT - A Case Study of Biomagnification Causing Extinction Risk of Herring Gull in Long Island'?

    <p>Herring Gull</p> Signup and view all the answers

    What is one of the biggest disadvantages of recycling e-waste in developing countries?

    <p>It can lead to the release of toxic pollutants into the environment. (A)</p> Signup and view all the answers

    The primary advantage of using ______ in a landfill is that it can destroy organic constituents in e-waste.

    <p>incineration</p> Signup and view all the answers

    Impurities in domestic sewage account for a significant percentage (over 10%) of the total wastewater.

    <p>False (B)</p> Signup and view all the answers

    How can suspended solids be removed from sewage water?

    <p>The suspended solids can be removed using a process called sedimentation or filtration.</p> Signup and view all the answers

    Match the following types of impurities in domestic sewage with their examples:

    <p>Suspended solids = Sand, silt, clay Colloidal material = Faecal matter, bacteria Dissolved materials = Nitrates, ammonia, phosphate, sodium, calcium</p> Signup and view all the answers

    If untreated domestic sewage is released into a pond or lake, what is ONE possible impact it can have on the water quality?

    <p>It can lead to the depletion of dissolved oxygen in the water body.</p> Signup and view all the answers

    Describe how the extinction of the Herring Gull, a top predator, might impact the food chain.

    <p>The extinction of the Herring Gull would disrupt the food chain by causing an overpopulation of the prey species that the Herring Gull feeds on. This imbalance could lead to cascading effects throughout the ecosystem, potentially impacting other species as well.</p> Signup and view all the answers

    Individuals with cystic fibrosis exhibit a recessive pattern of inheritance.

    <p>True (A)</p> Signup and view all the answers

    Which of the following symptoms are associated with cystic fibrosis?

    <p>All of the above (D)</p> Signup and view all the answers

    Match the following terms related to cystic fibrosis with their descriptions.

    <p>cf gene = The gene responsible for cystic fibrosis Recessive inheritance = A pattern of inheritance where two copies of the defective gene are required for the condition to manifest Mucus buildup = Accumulation of thick, sticky mucus in the lungs, pancreas, and other organs Transport channels = Channels that regulate the movement of chloride ions across cell membranes</p> Signup and view all the answers

    What is the genotype of individual II-2 in the pedigree chart?

    <p>Cc</p> Signup and view all the answers

    What is the category of disorders caused by an abnormal number of chromosomes?

    <p>Chromosomal disorders (D)</p> Signup and view all the answers

    Individuals with an extra chromosome 21 will have Down syndrome.

    <p>True (A)</p> Signup and view all the answers

    A color blind male and a female with normal vision can have a child who is a carrier for color blindness.

    <p>True (A)</p> Signup and view all the answers

    Rett syndrome is a genetic disorder caused by a mutation in the ______ gene.

    <p>MECP2</p> Signup and view all the answers

    What is the difference in the chromosomal makeup of individuals with Klinefelter's syndrome and Turner's syndrome?

    <p>Klinefelter's syndrome occurs due to an additional copy of the X chromosome (XXY), while Turner's syndrome results from the absence of one X chromosome (XO).</p> Signup and view all the answers

    Match the following chromosomal disorders with their characteristics:

    <p>Klinefelter's syndrome = Presence of an additional X chromosome Turner's syndrome = Absence of one X chromosome Rett syndrome = Mutation in the MECP2 gene Color blindness = Recessive allele on the X chromosome</p> Signup and view all the answers

    A female with Rett syndrome and a healthy male have a child. Which of the following statements is true about the child's likelihood of inheriting Rett syndrome?

    <p>The son will not have Rett syndrome, and the daughter will have Rett syndrome. (D)</p> Signup and view all the answers

    During meiosis, all daughter cells receive half the number of chromosomes from the parent cell.

    <p>True (A)</p> Signup and view all the answers

    The process of ______ is responsible for the formation of gametes, which have half the number of chromosomes as the parent cell.

    <p>meiosis</p> Signup and view all the answers

    What is the outcome of a cross between two individuals who each produce 8 different gametes?

    <p>64 offsprings (C)</p> Signup and view all the answers

    What are the two main differences between aneuploidy and polyploidy regarding chromosome number?

    <p>Aneuploidy is the loss or gain of one or a few chromosomes, while polyploidy is the gain of an entire set of chromosomes.</p> Signup and view all the answers

    Match the following genetic terms with their corresponding definitions:

    <p>Aneuploidy = The gain of an entire chromosome set(s) Polyploidy = The loss or gain of one or a few chromosomes Gamete = A haploid cell involved in sexual reproduction Mitosis = A type of cell division that produces two identical daughter cells</p> Signup and view all the answers

    Which of the following statements is TRUE about X-linked recessive transmission?

    <p>Carrier females have a 50% chance of passing the trait to their sons. (C)</p> Signup and view all the answers

    Explain why a color blind male will have a carrier but normal vision daughter if his mother is a carrier.

    <p>The color blind father contributes a Y chromosome and the carrier mother contributes her normal X chromosome to the daughter, making her a carrier but with normal vision.</p> Signup and view all the answers

    Which stage of cell division does polyploidy typically occur?

    <p>Anaphase/Cytokinesis (A)</p> Signup and view all the answers

    What are the possible blood groups of a child if one parent has the genotype IAi and the other parent has the genotype IBi?

    <p>IAi, IBi, IAIB, ii (D)</p> Signup and view all the answers

    A child with the blood group ii can inherit the IA allele from their mother and the IB allele from their father.

    <p>False (B)</p> Signup and view all the answers

    What is the genotype of a person with blood group B?

    <p>IBi or IBIB</p> Signup and view all the answers

    The Punnett square shows that the offspring of a cross between XhXh and XHY individuals have a ______ chance of inheriting the recessive allele Xh.

    <p>50%</p> Signup and view all the answers

    Match the genotype with the corresponding phenotype for offspring in the Punnett square provided.

    <p>XHXh = Carrier female XhXh = Female with muscular dystrophy XHY = Normal male XhY = Male with muscular dystrophy</p> Signup and view all the answers

    The phenotypic ratio for the cross shown in the Punnett square is 1:1:1:1.

    <p>True (A)</p> Signup and view all the answers

    Which genotype(s) would result in a purple axial flower in a plant with alleles for flower color (W for white and w for purple) and flower position (T for terminal and t for axial)?

    <p>wwTT and wwTt (C)</p> Signup and view all the answers

    To determine the genotype of a plant with purple axial flowers, what type of cross should be performed?

    <p>Test cross</p> Signup and view all the answers

    Flashcards

    Recombinant DNA Technology

    A method for combining DNA from different sources to create new genetic combinations.

    LacZ gene

    A gene that encodes for β-galactosidase, used as a reporter in recombinant DNA.

    β-galactosidase

    An enzyme that breaks down lactose and is produced by cells containing the LacZ gene.

    Competent cells

    Bacterial cells that are able to take up foreign DNA, often used in transformation.

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    DNA ligase

    An enzyme that joins DNA fragments by forming phosphodiester bonds.

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    Polymerase Chain Reaction (PCR)

    A technique to amplify DNA sequences by mimicking natural DNA replication.

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    Purpose of steam in bioprocessing

    Steam is used to maintain sterility in bioprocessing operations.

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    Genetic variation in rDNA

    Recombinant DNA technology introduces genetic variation similar to sexual reproduction.

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    Color Blindness Inheritance

    A color blind male passes the Y chromosome, mother passes normal vision trait to child, non-carrier.

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    Rett Syndrome Transmission

    A son will not have Rett syndrome; a daughter will have Rett syndrome.

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    Klinefelter's Syndrome

    A condition caused by an extra X chromosome, affecting males primarily.

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    Turner's Syndrome

    A condition caused by the absence of one X chromosome, affecting females only.

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    Chromosomal Disorders Similarities

    Both Klinefelter's and Turner's syndromes are related to sex chromosomes and result in sterility.

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    Cystic fibrosis

    A genetic condition causing mucus buildup in organs.

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    cf gene

    Gene responsible for cystic fibrosis production.

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    Pattern of inheritance

    The method by which traits are passed down generations.

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    Genotype of II-2

    Genetic makeup of individual II-2 in the pedigree.

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    Reproductive features of karyotype disorders

    Characteristic features affecting reproduction and development.

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    Physical features in karyotype disorders

    Distinct physical traits resulting from chromosomal abnormalities.

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    Category of disorders from karyotype

    Genetic disorders caused by chromosomal abnormalities.

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    Cause of karyotype disorders

    Due to errors in cell division producing abnormal numbers of chromosomes.

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    Herring Gull Extinction

    The extinction of Herring Gull affects ecosystem balance as it is a top predator.

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    Bio-magnification

    Bio-magnification refers to the increased concentration of toxins in organisms at higher trophic levels.

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    E-waste Recycling Disadvantages

    One disadvantage of e-waste recycling is exposure to hazardous materials, risking health and safety.

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    Incineration Advantage

    Incineration reduces waste volume and can generate energy from burning e-waste.

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    Suspended Solids Removal

    Suspended solids can be removed from sewage using sedimentation or filtration methods.

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    Colloidal Impurities Use

    Colloidal impurities can be utilized for composting or as fertilizers in agriculture.

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    Domestic Sewage Impact

    Untreated domestic sewage can lead to nutrient pollution, causing algae blooms and poor water quality.

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    Ecological Risk of DDT

    DDT poses ecological risks, such as the potential extinction of top predators like Herring Gulls due to bio-magnification.

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    Spermatogonium

    A germ cell that undergoes mitosis to produce sperm cells.

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    Meiosis

    A type of cell division resulting in four cells with half the chromosomes.

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    X-linked recessive inheritance

    A mode of inheritance where traits are passed via the X chromosome.

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    Aneuploidy

    The loss or gain of individual chromosomes in the cell.

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    Polyploidy

    The condition of having one or more extra sets of chromosomes.

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    Gamete Genotype

    The genetic constitution of reproductive cells (gametes).

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    Offspring Count

    Total number of offspring produced from a genetic cross.

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    Blood group genotypes

    A child's blood groups can be IAi, IBi, IAIB, or ii.

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    Parent genotypes for blood group

    The required parent genotypes for blood group inheritance are IAi and IBi.

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    Punnett square

    A diagram used to predict genotype and phenotype ratios of offspring.

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    Genotypic ratio from punnett square

    The resulting ratio from a cross is 1 XHXh : 1 XhXh : 1 XHY : 1 XhY.

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    Phenotypic ratio example

    1 female with muscular dystrophy : 1 carrier female : 1 male with muscular dystrophy : 1 normal male.

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    Test cross

    A cross between an individual with an unknown genotype and a homozygous recessive individual.

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    Cross with homozygous purple flowers

    Involves a test cross to determine genotype of the unknown flower.

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    Inference from flower cross

    If genotype is wwTt, then 50% progeny will have purple terminal flowers.

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    Study Notes

    Competency Focused Practice Questions

    • This document is a collection of competency-based practice questions for Biology (Volume 2), Grade 12, following the CBSE curriculum.
    • The questions are designed to assess conceptual understanding and application of biological concepts.
    • The questions include a mix of free response and multiple-choice questions.
    • All items are aligned to the NCERT curriculum and learning outcomes.
    • Multiple-choice questions have carefully constructed options (correct and distractors) meant to expose gaps in student learning.
    • Subjective questions include detailed scoring rubrics for evaluation.
    • The items are designed for teachers to use, gauge student learning, provide feedback and insights into learning gaps.
    • The document is co-created by CBSE Centre for Excellence in Assessment and Educational Initiatives (Ei).

    Contents

    • The document covers various biological topics, including biodiversity, conservation, biotechnology, ecosystems, environmental issues, human health and disease, microbes, molecular basis of inheritance, principles of inheritance, reproduction, and food production.
    • Each topic is associated with a dedicated answer key and marking scheme, adding to the resourcefulness of the book.

    Preface

    • Assessments are crucial tools to evaluate learning and offer feedback about instructional effectiveness and student understanding.
    • Competency-based assessments are emphasized, focusing on higher-order skills like analysis, critical thinking and problem-solving.
    • The document aims to help students learn with understanding and apply concepts to solve complex problems.
    • CBSE and Ei have collaborated on this project, drawing on resources such as Essential Concepts and A Question A Day.

    Biodiversity and Conservation

    • The content on Biodiversity and Conservation deals with related questions.
    • There are multiple choice questions and free response questions along with related answer keys and marking schemes.

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    Description

    Test your knowledge on recombinant DNA technology and its processes, including the role of enzymes and natural processes. The quiz covers various aspects like the function of β-galactosidase and DNA ligase, along with relevant ecological concerns. Prepare to delve into the critical steps and terminology of this advanced field.

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