3 Questions
What is the first step in the process of Recombinant DNA technology?
Obtaining a DNA fragment with the desired gene from a donor cell
What is the role of a restriction enzyme in Recombinant DNA technology?
Cutting both the DNA fragment and the plasmid at specific points
What is the function of DNA ligase in Recombinant DNA technology?
Forming a recombinant plasmid by joining the cut DNA fragment to the open plasmid
Study Notes
- Recombinant DNA technology is a method for transferring DNA from one organism to another, introducing new traits or characteristics
- Obtaining a DNA fragment with the desired gene from a donor cell is the first step
- An appropriate plasmid, commonly used as a vector to transfer the gene, is secured from a bacterium
- Restriction enzyme is used to cut both the DNA fragment and the plasmid at specific points
- DNA ligase joins the cut DNA fragment to the open plasmid, forming a recombinant plasmid
- This recombinant plasmid can then be introduced into a host cell to produce proteins of other species or genetically modified organisms with new traits.
Learn the fundamental steps of recombinant DNA technology, from obtaining a DNA fragment to introducing the recombinant plasmid into a host cell. Understand how this method is used to transfer DNA and introduce new traits in organisms.
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