RDT DNA Modifying Enzymes Quiz
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Questions and Answers

What is needed to avoid self-ligation in vector-vector ligation?

  • Use of two blunt-ended enzymes
  • Use of two sticky-ended enzymes
  • Incompatible vector ends
  • Compatible vector ends (correct)

In the context of DNA modifying enzymes, what is the purpose of using two enzymes with incompatible sticky ends?

  • To prevent ligation
  • To achieve directional ligation (correct)
  • To enhance DNA replication
  • To promote self-ligation

Which enzyme is used to chew away the single-stranded overhangs of DNA in a 3’ to 5’ direction?

  • Alkaline Phosphatase (correct)
  • Ligase enzyme
  • HindIII
  • BamHI

What type of reaction is used to polish the overhangs of DNA?

<p>Fill-in reaction (C)</p> Signup and view all the answers

What is the purpose of using linkers in DNA modifying procedures?

<p>To add specific sequences to DNA fragments (D)</p> Signup and view all the answers

Which component is necessary for a ligation reaction to be successful?

<p>Buffer (C)</p> Signup and view all the answers

What enzymatic action is involved in making DNA ends compatible during the chew-in reaction?

<p>Removing bases from overhangs (B)</p> Signup and view all the answers

Why are two enzymes, one blunt and one sticky, used together in some DNA modifying processes?

<p>To enhance ligation efficiency (B)</p> Signup and view all the answers

What is the importance of using ligase enzyme in a ligation reaction?

<p>To join DNA fragments together (B)</p> Signup and view all the answers

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