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Questions and Answers
What is the primary function of restriction endonucleases in DNA manipulation?
To cut DNA at specific sites called recognition sites.
What characteristic of Type II restriction endonucleases makes them ideal for gene cloning?
They cut in a predictable and consistent manner at a site within or adjacent to the recognition sequence.
What is the name of the cofactor required by Type II restriction endonucleases?
Magnesium ion (Mg++)
What is the typical length of recognition sequences for Type II restriction endonucleases?
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What is the significance of the name 'EcoRI' in the context of restriction endonucleases?
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What is the role of ligase in the process of recombinant DNA technology?
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What is the purpose of genetic fusion in genetic engineering?
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What is the result of protoplast fusion in genetic engineering?
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What is the role of plasmids in gene amplification?
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What is the application of rapid reproduction of bacteriophages in gene amplification?
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What is the purpose of using Ice-minus GE Ps.syringae in genetic engineering?
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What is the limitation of protoplast fusion in genetic engineering?
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What is the problem associated with the disposal of whey in the dairy industry, and how can genetically engineered X. campestris help solve this problem?
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What is the main goal of recombinant DNA technology in genetic engineering?
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What is the role of enzymes in recombinant DNA technology?
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What is the purpose of using vectors in genetic engineering?
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What is the outcome of combining DNA from different species using recombinant DNA technology?
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What is the significance of nucleotide recognition in recombinant DNA technology?
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