Recombinant DNA Diagnostics Quiz
16 Questions
2 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What are DNA microarrays sometimes called?

  • Genomic arrays
  • Gene slides
  • Nucleic acid plates
  • DNA chips (correct)
  • What is the size of DNA microarray supports typically compared to?

  • A pipette tip
  • A test tube
  • A petri dish
  • A microscope slide (correct)
  • What is the purpose of an expression microarray?

  • To synthesize gene-specific oligonucleotides
  • To detect single nucleotide polymorphisms
  • To measure gene expression (mRNA) (correct)
  • To compare genomic DNA samples
  • What does a comparative genomic microarray detect?

    <p>Loss or gain of genomic DNA</p> Signup and view all the answers

    How are the DNA samples attached to the support phase in a DNA microarray chip?

    <p>Spotted, printed, or synthesized directly</p> Signup and view all the answers

    What is the main technology underlying the development of DNA microarrays?

    <p>Genomics, PCR, and nucleic acid hybridization</p> Signup and view all the answers

    What is the purpose of the RT-PCR technique in COVID-19 detection?

    <p>To transcribe and amplify specific SARSCoV-2 genomic sequence</p> Signup and view all the answers

    Which body parts are samples taken from for COVID-19 detection using RT-PCR technique?

    <p>Nose and throat</p> Signup and view all the answers

    What does a lower Ct value in RT-PCR indicate?

    <p>Higher viral loads, indicating a positive COVID-19 case</p> Signup and view all the answers

    What is the purpose of the TaqMan probe in RT-PCR?

    <p>To release a fluorescent dye when attached to DNA strands</p> Signup and view all the answers

    How many cycles does RT-PCR usually go through?

    <p>35</p> Signup and view all the answers

    What does a higher Ct value in RT-PCR indicate?

    <p>Higher viral loads, indicating a positive COVID-19 case</p> Signup and view all the answers

    What is the main limitation of RT-PCR technique for COVID-19 detection mentioned in the text?

    <p>Possible cross contamination with other viral species</p> Signup and view all the answers

    What is measured by the computer during RT-PCR?

    <p>Amount of fluorescence in the sample after each cycle</p> Signup and view all the answers

    What is used to purify the sample taken for COVID-19 detection using RT-PCR?

    <p>Chemical solutions that remove substances such as proteins and fats</p> Signup and view all the answers

    What is tracked by the computer during RT-PCR?

    <p>Fluorescence in real time on the screen</p> Signup and view all the answers

    More Like This

    Clinical Genomics
    40 questions

    Clinical Genomics

    LogicalSquirrel avatar
    LogicalSquirrel
    Molecular Diagnostics in Medicine
    16 questions
    Diagnóstico Molecular en Salud
    37 questions
    Use Quizgecko on...
    Browser
    Browser