Recombinant DNA Diagnostics Quiz
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Questions and Answers

What are DNA microarrays sometimes called?

  • Genomic arrays
  • Gene slides
  • Nucleic acid plates
  • DNA chips (correct)
  • What is the size of DNA microarray supports typically compared to?

  • A pipette tip
  • A test tube
  • A petri dish
  • A microscope slide (correct)
  • What is the purpose of an expression microarray?

  • To synthesize gene-specific oligonucleotides
  • To detect single nucleotide polymorphisms
  • To measure gene expression (mRNA) (correct)
  • To compare genomic DNA samples
  • What does a comparative genomic microarray detect?

    <p>Loss or gain of genomic DNA</p> Signup and view all the answers

    How are the DNA samples attached to the support phase in a DNA microarray chip?

    <p>Spotted, printed, or synthesized directly</p> Signup and view all the answers

    What is the main technology underlying the development of DNA microarrays?

    <p>Genomics, PCR, and nucleic acid hybridization</p> Signup and view all the answers

    What is the purpose of the RT-PCR technique in COVID-19 detection?

    <p>To transcribe and amplify specific SARSCoV-2 genomic sequence</p> Signup and view all the answers

    Which body parts are samples taken from for COVID-19 detection using RT-PCR technique?

    <p>Nose and throat</p> Signup and view all the answers

    What does a lower Ct value in RT-PCR indicate?

    <p>Higher viral loads, indicating a positive COVID-19 case</p> Signup and view all the answers

    What is the purpose of the TaqMan probe in RT-PCR?

    <p>To release a fluorescent dye when attached to DNA strands</p> Signup and view all the answers

    How many cycles does RT-PCR usually go through?

    <p>35</p> Signup and view all the answers

    What does a higher Ct value in RT-PCR indicate?

    <p>Higher viral loads, indicating a positive COVID-19 case</p> Signup and view all the answers

    What is the main limitation of RT-PCR technique for COVID-19 detection mentioned in the text?

    <p>Possible cross contamination with other viral species</p> Signup and view all the answers

    What is measured by the computer during RT-PCR?

    <p>Amount of fluorescence in the sample after each cycle</p> Signup and view all the answers

    What is used to purify the sample taken for COVID-19 detection using RT-PCR?

    <p>Chemical solutions that remove substances such as proteins and fats</p> Signup and view all the answers

    What is tracked by the computer during RT-PCR?

    <p>Fluorescence in real time on the screen</p> Signup and view all the answers

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