16 Questions
What are DNA microarrays sometimes called?
DNA chips
What is the size of DNA microarray supports typically compared to?
A microscope slide
What is the purpose of an expression microarray?
To measure gene expression (mRNA)
What does a comparative genomic microarray detect?
Loss or gain of genomic DNA
How are the DNA samples attached to the support phase in a DNA microarray chip?
Spotted, printed, or synthesized directly
What is the main technology underlying the development of DNA microarrays?
Genomics, PCR, and nucleic acid hybridization
What is the purpose of the RT-PCR technique in COVID-19 detection?
To transcribe and amplify specific SARSCoV-2 genomic sequence
Which body parts are samples taken from for COVID-19 detection using RT-PCR technique?
Nose and throat
What does a lower Ct value in RT-PCR indicate?
Higher viral loads, indicating a positive COVID-19 case
What is the purpose of the TaqMan probe in RT-PCR?
To release a fluorescent dye when attached to DNA strands
How many cycles does RT-PCR usually go through?
35
What does a higher Ct value in RT-PCR indicate?
Higher viral loads, indicating a positive COVID-19 case
What is the main limitation of RT-PCR technique for COVID-19 detection mentioned in the text?
Possible cross contamination with other viral species
What is measured by the computer during RT-PCR?
Amount of fluorescence in the sample after each cycle
What is used to purify the sample taken for COVID-19 detection using RT-PCR?
Chemical solutions that remove substances such as proteins and fats
What is tracked by the computer during RT-PCR?
Fluorescence in real time on the screen
Test your knowledge on the utilization of recombinant DNA in diagnostics, including microarrays and PCR for COVID-19 detection. Learn about the principles and importance of microarray technology as a diagnostic tool.
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