12 Questions
What is the purpose of using RT-PCR technique in COVID-19 detection?
To transcribe and amplify specific SARSCoV-2 genomic sequence
What is the purpose of the Cycle threshold (Ct value) in RT-PCR?
To measure the amount of fluorescence in the sample after each cycle
What does a lower Ct value than 35 indicate in COVID-19 testing?
Positive COVID-19 case
What is a possible limitation of RT-PCR for COVID-19 detection mentioned in the text?
Possible cross-contamination with other viral species
What is the main purpose of DNA microarrays?
To measure gene expression (mRNA)
What is the size of typical DNA microarray supports?
The size of a microscope slide
What is the main application of comparative genomic microarrays?
To detect loss or gain of genomic DNA associated with certain genetic disorders
What is the main function of mutation microarrays?
To detect single nucleotide polymorphisms (SNPs)
Which technology allows the rapid and simultaneous screening of many thousands of genes?
DNA microarrays
What is the main material used to make DNA microarray supports?
Glass
Which type of DNA samples are spotted, printed, or synthesized directly on to the support phase?
Genomic DNA
Which technique is used for detecting loss or gain of genomic DNA associated with certain genetic disorders?
Comparative genomic microarray
Test your knowledge about the use of recombinant DNA in diagnostic tools such as microarrays and PCR. Learn about the principles and importance of microarrays, as well as the use of PCR in COVID-19 detection.
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