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Recombinant DNA in Diagnostics Quiz

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Questions and Answers

What is the purpose of using RT-PCR technique in COVID-19 detection?

To transcribe and amplify specific SARSCoV-2 genomic sequence (correct)

To measure the severity of the infection by counting the number of cycles

To purify the viral genetic material from the sample

To extract proteins and fats from the sample

What is the purpose of the Cycle threshold (Ct value) in RT-PCR?

To remove substances such as proteins and fats from the sample

To track the severity of the infection by measuring the number of cycles

To measure the amount of fluorescence in the sample after each cycle (correct)

To monitor the quality and technique followed in the kit used

What does a lower Ct value than 35 indicate in COVID-19 testing?

Positive COVID-19 case (correct)

A gender effect on the test results

Negative COVID-19 case

The presence of substances such as proteins and fats in the sample

What is a possible limitation of RT-PCR for COVID-19 detection mentioned in the text?

Possible cross-contamination with other viral species (D) Signup and view all the answers

What is the main purpose of DNA microarrays?

To measure gene expression (mRNA) (C) Signup and view all the answers

What is the size of typical DNA microarray supports?

The size of a microscope slide (B) Signup and view all the answers

What is the main application of comparative genomic microarrays?

To detect loss or gain of genomic DNA associated with certain genetic disorders (A) Signup and view all the answers

What is the main function of mutation microarrays?

To detect single nucleotide polymorphisms (SNPs) (A) Signup and view all the answers

Which technology allows the rapid and simultaneous screening of many thousands of genes?

DNA microarrays (A) Signup and view all the answers

What is the main material used to make DNA microarray supports?

Glass (B) Signup and view all the answers

Which type of DNA samples are spotted, printed, or synthesized directly on to the support phase?

Genomic DNA (B) Signup and view all the answers

Which technique is used for detecting loss or gain of genomic DNA associated with certain genetic disorders?

Comparative genomic microarray (D) Signup and view all the answers