14 Questions
Which of the following is NOT part of the normal process of cloning recombinant DNA in bacteria?
Separation of recombinant DNA by electrophoresis and use of Southern Blotting to determine where the desired recombinant DNA migrates
Which of the following statements about electrophoresis is INCORRECT?
All proteins are positively charged therefore they will move towards the cathode
The PCR technique involves the use of:
All of the above
In one form of familial isolated growth hormone deficiency, deletion of an exon of a gene on chromosome 17 alters the protein product. What is the consequence of this alteration in heterozygotes?
The altered protein product binds with the normal protein product encoded by the normal copy of the gene on chromosome 17
Which of the following genotypes causes Turner syndrome?
XX
The process of extravasation is:
The escape of a tumour cell from a blood or lymphatic vessel
Trisomy 21 means…
Having an extra chromosome 21
Unaffected parents of a child with cystic fibrosis attend a genetic counseling clinic when trying to decide whether they should have another baby. The likelihood of them having another child with cystic fibrosis is:
1 in 4
A person with type B blood could have which genotype type?
IB/IB or IB/i
Which of the following outcomes is possible?
Answers a and b
The failure of a chromosome in the segregation process during cell division is known as:
Chromosomal non-disjunction
Which of the following does NOT play a role in the regulation of transcription?
The spliceosome
The major cause of small-scale mutations in human cells is:
Errors arising during DNA replication
Which one of these is the most correct?
Nonsense mutation
Test your knowledge of the steps involved in cloning recombinant DNA in bacteria with this multiple choice quiz. Identify which step is NOT part of the normal cloning process.
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