Recombinant DNA Cloning Process Quiz
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Questions and Answers

Which of the following is NOT part of the normal process of cloning recombinant DNA in bacteria?

  • Production of recombinant DNA using DNA ligase and a mixture of digested cellular and plasmid DNA
  • Restriction endonuclease digestion of cellular and plasmid DNA
  • Transformation of bacteria by recombinant DNA plasmids and selection using antibiotics
  • Separation of recombinant DNA by electrophoresis and use of Southern Blotting to determine where the desired recombinant DNA migrates (correct)

    • Which of the following statements about electrophoresis is INCORRECT?

  • All proteins are positively charged therefore they will move towards the cathode (correct)
  • Negatively charged molecules will move towards the positively charged electrode (anode)
  • In gel electrophoresis molecules are separated by charge and size
  • Electrophoresis is the movement of charged molecules in an electrical field

    • The PCR technique involves the use of:

  • Synthesized oligonucleotide primers
  • DNA template
  • DNA polymerase
  • All of the above (correct)

    • In one form of familial isolated growth hormone deficiency, deletion of an exon of a gene on chromosome 17 alters the protein product. What is the consequence of this alteration in heterozygotes?

    <p>The altered protein product binds with the normal protein product encoded by the normal copy of the gene on chromosome 17</p> Signup and view all the answers

    Which of the following genotypes causes Turner syndrome?

    <p>XX</p> Signup and view all the answers

    The process of extravasation is:

    <p>The escape of a tumour cell from a blood or lymphatic vessel</p> Signup and view all the answers

    Trisomy 21 means…

    <p>Having an extra chromosome 21</p> Signup and view all the answers

    Unaffected parents of a child with cystic fibrosis attend a genetic counseling clinic when trying to decide whether they should have another baby. The likelihood of them having another child with cystic fibrosis is:

    <p>1 in 4</p> Signup and view all the answers

    A person with type B blood could have which genotype type?

    <p>IB/IB or IB/i</p> Signup and view all the answers

    Which of the following outcomes is possible?

    <p>Answers a and b</p> Signup and view all the answers

    The failure of a chromosome in the segregation process during cell division is known as:

    <p>Chromosomal non-disjunction</p> Signup and view all the answers

    Which of the following does NOT play a role in the regulation of transcription?

    <p>The spliceosome</p> Signup and view all the answers

    The major cause of small-scale mutations in human cells is:

    <p>Errors arising during DNA replication</p> Signup and view all the answers

    Which one of these is the most correct?

    <p>Nonsense mutation</p> Signup and view all the answers

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