Cloning and Recombinant DNA Technology
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Cloning and Recombinant DNA Technology

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What is the purpose of ligating the amplified fragment with a vector?

  • To express the foreign gene in the host cell
  • To produce a desirable protein
  • For further cloning (correct)
  • To introduce the recombinant DNA into the host cell

    • What makes recipient cells competent to receive DNA?

  • Making them transformation-competent (correct)
  • Making them antibiotic-resistant
  • Submitting them to high temperatures
  • Treating them with a special enzyme

    • Why do untransformed recipient cells die on agar plates containing ampicillin?

  • Because they are sensitive to ampicillin (correct)
  • Because they are not antibiotic-resistant
  • Because they do not have a selectable marker
  • Because they are not transformed

    • What is the function of a selectable marker?

    <p>To select transformed cells in the presence of an antibiotic</p> Signup and view all the answers

    Why is it necessary to multiply the alien DNA in a host cell?

    <p>To express the foreign gene</p> Signup and view all the answers

    What is the ultimate aim of most recombinant technologies?

    <p>To produce a desirable protein</p> Signup and view all the answers

    What is necessary for the expression of foreign genes in host cells?

    <p>Appropriate conditions</p> Signup and view all the answers

    What is the purpose of spreading transformed cells on agar plates containing ampicillin?

    <p>To select transformed cells</p> Signup and view all the answers

    What is a result of introducing a piece of alien DNA into a cloning vector and transferring it into a host cell?

    <p>The alien DNA gets multiplied</p> Signup and view all the answers

    Why is it necessary to understand technical details in recombinant technologies?

    <p>To express foreign genes in host cells</p> Signup and view all the answers

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