10 Questions
What is the purpose of ligating the amplified fragment with a vector?
For further cloning
What makes recipient cells competent to receive DNA?
Making them transformation-competent
Why do untransformed recipient cells die on agar plates containing ampicillin?
Because they are sensitive to ampicillin
What is the function of a selectable marker?
To select transformed cells in the presence of an antibiotic
Why is it necessary to multiply the alien DNA in a host cell?
To express the foreign gene
What is the ultimate aim of most recombinant technologies?
To produce a desirable protein
What is necessary for the expression of foreign genes in host cells?
Appropriate conditions
What is the purpose of spreading transformed cells on agar plates containing ampicillin?
To select transformed cells
What is a result of introducing a piece of alien DNA into a cloning vector and transferring it into a host cell?
The alien DNA gets multiplied
Why is it necessary to understand technical details in recombinant technologies?
To express foreign genes in host cells
Learn about the process of cloning and recombinant DNA technology, including the role of the origin of replication in DNA multiplication. Understand how alien DNA can be linked with the origin of replication to replicate and multiply itself in a host organism.
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