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Cloning and Recombinant DNA Technology

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Questions and Answers

What is the purpose of ligating the amplified fragment with a vector?

To express the foreign gene in the host cell

To produce a desirable protein

For further cloning (correct)

To introduce the recombinant DNA into the host cell

What makes recipient cells competent to receive DNA?

Making them transformation-competent (correct)

Making them antibiotic-resistant

Submitting them to high temperatures

Treating them with a special enzyme

Why do untransformed recipient cells die on agar plates containing ampicillin?

Because they are sensitive to ampicillin (correct)

Because they are not antibiotic-resistant

Because they do not have a selectable marker

Because they are not transformed

What is the function of a selectable marker?

To select transformed cells in the presence of an antibiotic Signup and view all the answers

Why is it necessary to multiply the alien DNA in a host cell?

To express the foreign gene Signup and view all the answers

What is the ultimate aim of most recombinant technologies?

To produce a desirable protein Signup and view all the answers

What is necessary for the expression of foreign genes in host cells?

Appropriate conditions Signup and view all the answers

What is the purpose of spreading transformed cells on agar plates containing ampicillin?

To select transformed cells Signup and view all the answers

What is a result of introducing a piece of alien DNA into a cloning vector and transferring it into a host cell?

The alien DNA gets multiplied Signup and view all the answers

Why is it necessary to understand technical details in recombinant technologies?

To express foreign genes in host cells Signup and view all the answers