Podcast
Questions and Answers
What is the purpose of ligating the amplified fragment with a vector?
What is the purpose of ligating the amplified fragment with a vector?
- To express the foreign gene in the host cell
- To produce a desirable protein
- For further cloning (correct)
- To introduce the recombinant DNA into the host cell
What makes recipient cells competent to receive DNA?
What makes recipient cells competent to receive DNA?
- Making them transformation-competent (correct)
- Making them antibiotic-resistant
- Submitting them to high temperatures
- Treating them with a special enzyme
Why do untransformed recipient cells die on agar plates containing ampicillin?
Why do untransformed recipient cells die on agar plates containing ampicillin?
- Because they are sensitive to ampicillin (correct)
- Because they are not antibiotic-resistant
- Because they do not have a selectable marker
- Because they are not transformed
What is the function of a selectable marker?
What is the function of a selectable marker?
Why is it necessary to multiply the alien DNA in a host cell?
Why is it necessary to multiply the alien DNA in a host cell?
What is the ultimate aim of most recombinant technologies?
What is the ultimate aim of most recombinant technologies?
What is necessary for the expression of foreign genes in host cells?
What is necessary for the expression of foreign genes in host cells?
What is the purpose of spreading transformed cells on agar plates containing ampicillin?
What is the purpose of spreading transformed cells on agar plates containing ampicillin?
What is a result of introducing a piece of alien DNA into a cloning vector and transferring it into a host cell?
What is a result of introducing a piece of alien DNA into a cloning vector and transferring it into a host cell?
Why is it necessary to understand technical details in recombinant technologies?
Why is it necessary to understand technical details in recombinant technologies?
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