Recombinant DNA (rDNA)
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Questions and Answers

What is the main characteristic of recombinant DNA?

  • It is formed by laboratory methods of genetic recombination (correct)
  • It can only be created using palindromic sequences
  • It always contains genetic material from two different species
  • It has a different chemical structure from natural DNA

What distinguishes the DNA molecules of different organisms?

  • Their length of DNA strands
  • Their resistance to recombination
  • Their chemical structure (correct)
  • Their ability to form palindromic sequences

What is the outcome of using recombinant DNA technology?

  • Creating new species with unique genetic material
  • Isolating and cloning a gene or DNA segment into an indefinite number of identical copies (correct)
  • Producing DNA with chimeric characteristics
  • Modifying the chemical structure of DNA

What is the purpose of introducing recombinant DNA molecules into host cells?

<p>To propagate and multiply the new combinations of genetic material (C)</p> Signup and view all the answers

What is the role of a vector in the process of making recombinant DNA?

<p>Carrying the DNA molecule into the host cell (A)</p> Signup and view all the answers

How is bacterial plasmid DNA commonly obtained for use as a vector?

<p>Through ultracentrifugation in a cesium chloride density gradient (B)</p> Signup and view all the answers

What is the function of restriction enzymes in the process of making recombinant DNA?

<p>Cutting the DNA molecule at specific target sequences (D)</p> Signup and view all the answers

How are phages used as vectors for cloning DNA in bacterial systems?

<p>By introducing it through infection of a bacterial lawn growing on an agar plate (A)</p> Signup and view all the answers

What is the purpose of ligase enzyme in making recombinant DNA?

<p>Joining vector DNA with the gene of interest (A)</p> Signup and view all the answers

How is vector DNA introduced into a host cell in the case of plasmid vectors?

<p>By introducing it into a flask containing a culture of E.coli (D)</p> Signup and view all the answers

Flashcards

Recombinant DNA

A DNA molecule created by combining DNA from different sources in a laboratory setting.

What distinguishes DNA molecules?

The specific sequence of nucleotides within a DNA molecule.

Outcome of recombinant DNA technology

The process of producing multiple identical copies of a specific gene or DNA segment.

Purpose of introducing recombinant DNA

To introduce recombinant DNA molecules into host cells so they can multiply and produce the desired product.

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What is a vector in recombinant DNA?

A carrier that delivers the recombinant DNA molecule into the host cell.

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How bacterial plasmid DNA is obtained

A method used to isolate bacterial plasmid DNA, utilizing differences in density.

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Function of restriction enzymes

Enzymes that cut DNA molecules at specific target sequences, creating fragments.

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Phages as vectors

Using bacteriophages to introduce recombinant DNA into bacteria.

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Purpose of ligase enzyme

An enzyme that joins the ends of vector DNA with the gene of interest, creating a complete recombinant molecule.

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Introducing vector DNA into a host cell

A method used to introduce plasmid vectors into bacterial cells.

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