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Recombinant DNA (rDNA)

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Questions and Answers

What is the main characteristic of recombinant DNA?

It is formed by laboratory methods of genetic recombination (correct)

It can only be created using palindromic sequences

It always contains genetic material from two different species

It has a different chemical structure from natural DNA

What distinguishes the DNA molecules of different organisms?

Their length of DNA strands

Their resistance to recombination

Their chemical structure (correct)

Their ability to form palindromic sequences

What is the outcome of using recombinant DNA technology?

Creating new species with unique genetic material

Isolating and cloning a gene or DNA segment into an indefinite number of identical copies (correct)

Producing DNA with chimeric characteristics

Modifying the chemical structure of DNA

What is the purpose of introducing recombinant DNA molecules into host cells?

To propagate and multiply the new combinations of genetic material Signup and view all the answers

What is the role of a vector in the process of making recombinant DNA?

Carrying the DNA molecule into the host cell Signup and view all the answers

How is bacterial plasmid DNA commonly obtained for use as a vector?

Through ultracentrifugation in a cesium chloride density gradient Signup and view all the answers

What is the function of restriction enzymes in the process of making recombinant DNA?

Cutting the DNA molecule at specific target sequences Signup and view all the answers

How are phages used as vectors for cloning DNA in bacterial systems?

By introducing it through infection of a bacterial lawn growing on an agar plate Signup and view all the answers

What is the purpose of ligase enzyme in making recombinant DNA?

Joining vector DNA with the gene of interest Signup and view all the answers

How is vector DNA introduced into a host cell in the case of plasmid vectors?

By introducing it into a flask containing a culture of E.coli Signup and view all the answers