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Questions and Answers
Kastamonu garlic clone is suitable for summer consumption
Kastamonu garlic clone is suitable for summer consumption
False
Tunceli garlic is common in multiple provinces of Turkey
Tunceli garlic is common in multiple provinces of Turkey
False
The garlic production areas and seedling production areas are separated in Turkey
The garlic production areas and seedling production areas are separated in Turkey
False
Nematode is one of the most important diseases and pests affecting garlic production in Turkey
Nematode is one of the most important diseases and pests affecting garlic production in Turkey
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The white rot disease does not affect garlic production in Turkey
The white rot disease does not affect garlic production in Turkey
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The most common viruses affecting garlic production in Turkey are OYDV and LYSV
The most common viruses affecting garlic production in Turkey are OYDV and LYSV
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The viruses OYDV and LYSV together result in a yield loss of up to 50% in garlic production in Turkey
The viruses OYDV and LYSV together result in a yield loss of up to 50% in garlic production in Turkey
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Real-time PCR method is less sensitive and specific than other techniques for identifying plant viruses.
Real-time PCR method is less sensitive and specific than other techniques for identifying plant viruses.
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The meristem culture technique is widely used for obtaining virus-free plants in garlic production.
The meristem culture technique is widely used for obtaining virus-free plants in garlic production.
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Extracting the meristem regions of the plant is easy and requires minimal expertise.
Extracting the meristem regions of the plant is easy and requires minimal expertise.
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Nucleic acid production capacity in meristematic tissue during cell division is used for the formation of virus particles.
Nucleic acid production capacity in meristematic tissue during cell division is used for the formation of virus particles.
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The study evaluated the micropropagation capacity of A. tuncelianum, an endemic species of Turkey, and A. sativum, the Kastamonu garlic clone.
The study evaluated the micropropagation capacity of A. tuncelianum, an endemic species of Turkey, and A. sativum, the Kastamonu garlic clone.
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Transportation of viruses to the meristem region of the plant is prevented due to the lack of transport system in meristem.
Transportation of viruses to the meristem region of the plant is prevented due to the lack of transport system in meristem.
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Real-time PCR method was found to be more sensitive than other methods in the diagnosis of potato virus Y (PVY).
Real-time PCR method was found to be more sensitive than other methods in the diagnosis of potato virus Y (PVY).
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Two different nutrient media were tested in terms of micropropagation success, with Medium 2 found to be more successful than Medium 1.
Two different nutrient media were tested in terms of micropropagation success, with Medium 2 found to be more successful than Medium 1.
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There is an effective chemical application available against virus control in garlic production in Turkey.
There is an effective chemical application available against virus control in garlic production in Turkey.
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Meristem culture technique is the most common method for obtaining virus-free plants
Meristem culture technique is the most common method for obtaining virus-free plants
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Shoot tip culture technique is sufficient for all cases of virus-free plant propagation
Shoot tip culture technique is sufficient for all cases of virus-free plant propagation
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Real-time PCR assay can provide quantitative assessment of viruses in plants with high efficiency and very low chances of infection
Real-time PCR assay can provide quantitative assessment of viruses in plants with high efficiency and very low chances of infection
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The study was conducted at the University of Cukurova Turkey and Adana Veterinary Control Institute between 2009 and 2010
The study was conducted at the University of Cukurova Turkey and Adana Veterinary Control Institute between 2009 and 2010
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Allium sativum (Kastamonu garlic clone) and Allium tuncelianum garlic species infected with viruses were used as plant material
Allium sativum (Kastamonu garlic clone) and Allium tuncelianum garlic species infected with viruses were used as plant material
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Sixty in vitro plants were used for real-time PCR studies after 6 weeks of micropropagation
Sixty in vitro plants were used for real-time PCR studies after 6 weeks of micropropagation
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The qualitative detection program was not used for evaluation in the Roche LightCycler 2.0 Real-time PCR
The qualitative detection program was not used for evaluation in the Roche LightCycler 2.0 Real-time PCR
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Real-time PCR was carried out using 600s at 95°C for denaturation, 45 cycles of 10s at 95°C, 30s at 60°C, 2m at 72°C, and 30m at 40°C for cooling.
Real-time PCR was carried out using 600s at 95°C for denaturation, 45 cycles of 10s at 95°C, 30s at 60°C, 2m at 72°C, and 30m at 40°C for cooling.
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The primers used for OYDV and LYSV viruses were not registered in GenBank and their sequences were not specified.
The primers used for OYDV and LYSV viruses were not registered in GenBank and their sequences were not specified.
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Ninety meristems and ninety shoot tips were used in the experiment for A. tuncelianum.
Ninety meristems and ninety shoot tips were used in the experiment for A. tuncelianum.
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Meristem cultures resulted in 84 healthy plants, 1 unhealthy plant, and 5 infected plants for A. tuncelianum.
Meristem cultures resulted in 84 healthy plants, 1 unhealthy plant, and 5 infected plants for A. tuncelianum.
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The plantlets obtained from meristem cultures showed better micropropagation results in Medium 2 compared to Medium 1 for A. tuncelianum.
The plantlets obtained from meristem cultures showed better micropropagation results in Medium 2 compared to Medium 1 for A. tuncelianum.
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In the first micropropagation of A. sativum, 89 meristems and 87 shoot tips were used.
In the first micropropagation of A. sativum, 89 meristems and 87 shoot tips were used.
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The study concluded that shoot tip culture was more effective than meristem culture in obtaining virus-free plantlets.
The study concluded that shoot tip culture was more effective than meristem culture in obtaining virus-free plantlets.
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Study Notes
- A mixture was prepared for real-time PCR detection of OYDV and LYSV viruses in A. tuncelianum using SYBER Green. The mixture contained isolated DNA, SYBER Green master, primers, Mg, and water.
- The real-time PCR was carried out using 600s at 95°C for denaturation, 45 cycles of 10s at 95°C, 30s at 60°C, 2m at 72°C, and 30m at 40°C for cooling.
- The primers used for OYDV and LYSV viruses were registered in GenBank and their sequences were specified.
- Ninety meristems and ninety shoot tips were used in the experiment for A. tuncelianum. Meristems took 8 weeks to grow into plants, while shoot tips took 3-4 weeks.
- Meristem cultures resulted in 84 healthy plants, 1 unhealthy plant, and 5 infected plants. Shoot tip cultures resulted in 80 healthy plants, 1 unhealthy plant, and 9 infected plants.
- The plantlets obtained from meristem cultures showed better micropropagation results in Medium 2 compared to Medium 1. In the first micropropagation, 14.1 shoots/plant and 4.63 shoots/plant were obtained from Medium 2 and Medium 1, respectively.
- In the first micropropagation of A. sativum, 89 meristems and 87 shoot tips were used. Meristem cultures resulted in 89 healthy plants and 1 unhealthy plant. Shoot tip cultures resulted in 87 healthy plants and 3 infected plants.
- The micropropagation results showed that Medium 2 was more successful than Medium 1 in both garlic species.
- Sixty garlic plants were tested for OYDV and LYSV viruses using real-time PCR. No viruses were detected in meristem cultures of both garlic species. Viruses were detected in shoot tip cultures of both garlic species, with OYDV detected in 80% of tested plants and LYSV in 67% of tested plants for A. tuncelianum, and 73% and 87% for A. sativum, respectively.
- The study concluded that meristem culture was more effective than shoot tip culture in obtaining virus-free plantlets.
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Description
Learn about the preparation of a real-time PCR mixture for DNA detection using SYBER Green master and the protocol for the detection process. This quiz covers the components of the mixture, the PCR protocol, and the use of SYBER Green for DNA detection.
