Molecular Biology and Diagnostics: PCR Clean-up Methods

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Enzymatic approach is faster, easier, and more affordable compared to ______ purification methods

bead/column-based

Enzymatic approach reduces cross-contamination of samples and lab material wastes such as tips and tubes, usually involving one single ______ step

pipetting

The 1st incubation in the enzymatic approach digests the excess ______ and dNTPs

primers

The 2nd incubation in the enzymatic approach inactivates enzymes at ______ temperature

<p>high</p> Signup and view all the answers

Enzymatic approach involves two enzymes, Phosphatase that removes leftover ______

<p>dNTPs</p> Signup and view all the answers

Enzymatic approach involves Exonuclease that removes leftover ______

<p>primers</p> Signup and view all the answers

A commercially available kit for enzymatic approach is called ______

<p>Exo-CIP</p> Signup and view all the answers

AccuPrep® PCR PURIFICATION KIT is a ______-based PCR Cleanup kit

<p>column</p> Signup and view all the answers

AccuPrep® PCR PURIFICATION KIT can purify up to 10μg of DNA fragments from PCR or other enzymatic reaction mixtures in ______ minutes

<p>5</p> Signup and view all the answers

1μL of Exo-CIP tube A and 1μL of Exo-CIP tube B are added to the PCR product to degrade excess primers and ______

<p>dNTPs</p> Signup and view all the answers

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