12 Questions
What is the function of Phytohemagglutinin in the context provided?
Stimulate T-lymphocytes to division and growth
Which reagent is used to prevent the formation of spindle fibers?
Colcemide
In Karyotyping, what does the use of a hypotonic solution aim to achieve?
Bulge cells
Which tube is specifically designed for chromosomal analysis during blood sampling in the context provided?
Green-covered lithium heparin and sodium heparin tube
What is the purpose of adding colcemide to the blood culture tubes during harvesting?
Breakdown spindle fibers
What is the function of trypsin in the context provided?
Digest chromosome proteins before staining
After adding the fixative solution, what is the next step in the process?
Discard the supernatant
What is the purpose of adding fixative to the cell suspension?
To maintain cell structure and prevent degradation
What is the purpose of using a pasture pipette to drop the cell suspension on a slide?
To evenly distribute cell suspension for staining
What is the function of centrifuging the tube at 1500 rpm for 10 minutes?
To create a cloudy pellet of cells
What does the 1:4 V/V ratio refer to in the preparation of Giemsa stain?
Ratio of Giemsa stain to Sorenson's buffer
What is the significance of leaving glass slides to dry for 24-48 hours before trypsin treatment?
To prepare for chromosome banding
Test your knowledge on the functions of various reagents used in cell biology, such as Phytohemagglutinin, Colcemide, Hypotonic Solution, Fixative, and Trypsin. Explore the methods involved in Karyotyping including blood sampling procedures.
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