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Reagent Functions and Karyotyping Methods Quiz

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Reagent Functions and Karyotyping Methods Quiz

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Questions and Answers

What is the function of Phytohemagglutinin in the context provided?

Bulge cells

Stimulate T-lymphocytes to division and growth (correct)

Breakdown formed spindle fibers

Prevent formation of spindle fibers

Which reagent is used to prevent the formation of spindle fibers?

Trypsin

Fixative

Colcemide (correct)

Hypotonic Solution

In Karyotyping, what does the use of a hypotonic solution aim to achieve?

Bulge cells (correct)

Remove red blood cells

Digest chromosome proteins

Prevent formation of spindle fibers

Which tube is specifically designed for chromosomal analysis during blood sampling in the context provided?

Green-covered lithium heparin and sodium heparin tube Signup and view all the answers

What is the purpose of adding colcemide to the blood culture tubes during harvesting?

Breakdown spindle fibers Signup and view all the answers

What is the function of trypsin in the context provided?

Digest chromosome proteins before staining Signup and view all the answers

After adding the fixative solution, what is the next step in the process?

Discard the supernatant Signup and view all the answers

What is the purpose of adding fixative to the cell suspension?

To maintain cell structure and prevent degradation Signup and view all the answers

What is the purpose of using a pasture pipette to drop the cell suspension on a slide?

To evenly distribute cell suspension for staining Signup and view all the answers

What is the function of centrifuging the tube at 1500 rpm for 10 minutes?

To create a cloudy pellet of cells Signup and view all the answers

What does the 1:4 V/V ratio refer to in the preparation of Giemsa stain?

Ratio of Giemsa stain to Sorenson's buffer Signup and view all the answers

What is the significance of leaving glass slides to dry for 24-48 hours before trypsin treatment?

To prepare for chromosome banding Signup and view all the answers