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Questions and Answers
What is the function of Phytohemagglutinin in the context provided?
What is the function of Phytohemagglutinin in the context provided?
- Bulge cells
- Stimulate T-lymphocytes to division and growth (correct)
- Breakdown formed spindle fibers
- Prevent formation of spindle fibers
Which reagent is used to prevent the formation of spindle fibers?
Which reagent is used to prevent the formation of spindle fibers?
- Trypsin
- Fixative
- Colcemide (correct)
- Hypotonic Solution
In Karyotyping, what does the use of a hypotonic solution aim to achieve?
In Karyotyping, what does the use of a hypotonic solution aim to achieve?
- Bulge cells (correct)
- Remove red blood cells
- Digest chromosome proteins
- Prevent formation of spindle fibers
Which tube is specifically designed for chromosomal analysis during blood sampling in the context provided?
Which tube is specifically designed for chromosomal analysis during blood sampling in the context provided?
What is the purpose of adding colcemide to the blood culture tubes during harvesting?
What is the purpose of adding colcemide to the blood culture tubes during harvesting?
What is the function of trypsin in the context provided?
What is the function of trypsin in the context provided?
After adding the fixative solution, what is the next step in the process?
After adding the fixative solution, what is the next step in the process?
What is the purpose of adding fixative to the cell suspension?
What is the purpose of adding fixative to the cell suspension?
What is the purpose of using a pasture pipette to drop the cell suspension on a slide?
What is the purpose of using a pasture pipette to drop the cell suspension on a slide?
What is the function of centrifuging the tube at 1500 rpm for 10 minutes?
What is the function of centrifuging the tube at 1500 rpm for 10 minutes?
What does the 1:4 V/V ratio refer to in the preparation of Giemsa stain?
What does the 1:4 V/V ratio refer to in the preparation of Giemsa stain?
What is the significance of leaving glass slides to dry for 24-48 hours before trypsin treatment?
What is the significance of leaving glass slides to dry for 24-48 hours before trypsin treatment?
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