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Function of Reagents and Karyotyping Method Quiz

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Questions and Answers

What is the function of Colcemide?

Prevent formation of spindle fibers and breakdown formed fibers

What is the purpose of using Hypotonic Solution in cell bulging?

To bulge cells

Why is Trypsin used in the Karyotyping process?

For chromosome digestion protein before staining

What is the function of Fixative in the process?

Remove red blood cell Signup and view all the answers

Why is 1-Phytohemagglutinin used?

To stimulate T-lymphocytes to division and growth Signup and view all the answers

What is the function of 1-Phytohemagglutinin in the Karyotyping process?

Stimulate T-lymphocytes to division and growth Signup and view all the answers

What is the role of Colcemide in the Karyotyping process?

Prevent formation of spindle fibers and breakdown existing fibers Signup and view all the answers

Why is Hypotonic Solution used in the Karyotyping process?

To bulge cells Signup and view all the answers

What is the function of Fixative in the Karyotyping process?

Remove red blood cells Signup and view all the answers

What should be the blood collection process for chromosomal analysis?

Venous blood in green-covered lithium heparin and sodium heparin tube Signup and view all the answers

Why is Trypsin used before staining in the Karyotyping process?

For chromosome digestion protein Signup and view all the answers

What is the purpose of fixing the cell suspension with methanol and glacial acetic acid?

To preserve the cell structure and prevent degradation. Signup and view all the answers

How is the cell suspension prepared on a slide for staining?

By dropping 3 to 4 drops of the suspension from a pasture pipette onto a wet, chilled, and grease-free slide. Signup and view all the answers

What staining solution is used after preparing the slide with the cell suspension?

Giemsa stain and Sorenson's buffer in a 1:4 ratio. Signup and view all the answers

How long are the glass slides left to dry after staining before trypsin treatment?

24 - 48 hours at room temperature. Signup and view all the answers

What is the significance of adding trypsin solution in the karyotyping process?

To facilitate banding patterns for better chromosome visualization. Signup and view all the answers

What does the presence of symbols like 't', 'B', 'R', 'fra', 'Del', or 'DC' indicate in a karyotype?

They represent different chromosomal structural abnormalities. Signup and view all the answers

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