Podcast
Questions and Answers
What is the function of Colcemide?
What is the function of Colcemide?
Prevent formation of spindle fibers and breakdown formed fibers
What is the purpose of using Hypotonic Solution in cell bulging?
What is the purpose of using Hypotonic Solution in cell bulging?
To bulge cells
Why is Trypsin used in the Karyotyping process?
Why is Trypsin used in the Karyotyping process?
For chromosome digestion protein before staining
What is the function of Fixative in the process?
What is the function of Fixative in the process?
Signup and view all the answers
Why is 1-Phytohemagglutinin used?
Why is 1-Phytohemagglutinin used?
Signup and view all the answers
What is the function of 1-Phytohemagglutinin in the Karyotyping process?
What is the function of 1-Phytohemagglutinin in the Karyotyping process?
Signup and view all the answers
What is the role of Colcemide in the Karyotyping process?
What is the role of Colcemide in the Karyotyping process?
Signup and view all the answers
Why is Hypotonic Solution used in the Karyotyping process?
Why is Hypotonic Solution used in the Karyotyping process?
Signup and view all the answers
What is the function of Fixative in the Karyotyping process?
What is the function of Fixative in the Karyotyping process?
Signup and view all the answers
What should be the blood collection process for chromosomal analysis?
What should be the blood collection process for chromosomal analysis?
Signup and view all the answers
Why is Trypsin used before staining in the Karyotyping process?
Why is Trypsin used before staining in the Karyotyping process?
Signup and view all the answers
What is the purpose of fixing the cell suspension with methanol and glacial acetic acid?
What is the purpose of fixing the cell suspension with methanol and glacial acetic acid?
Signup and view all the answers
How is the cell suspension prepared on a slide for staining?
How is the cell suspension prepared on a slide for staining?
Signup and view all the answers
What staining solution is used after preparing the slide with the cell suspension?
What staining solution is used after preparing the slide with the cell suspension?
Signup and view all the answers
How long are the glass slides left to dry after staining before trypsin treatment?
How long are the glass slides left to dry after staining before trypsin treatment?
Signup and view all the answers
What is the significance of adding trypsin solution in the karyotyping process?
What is the significance of adding trypsin solution in the karyotyping process?
Signup and view all the answers
What does the presence of symbols like 't', 'B', 'R', 'fra', 'Del', or 'DC' indicate in a karyotype?
What does the presence of symbols like 't', 'B', 'R', 'fra', 'Del', or 'DC' indicate in a karyotype?
Signup and view all the answers