17 Questions
What is the function of Colcemide?
Prevent formation of spindle fibers and breakdown formed fibers
What is the purpose of using Hypotonic Solution in cell bulging?
To bulge cells
Why is Trypsin used in the Karyotyping process?
For chromosome digestion protein before staining
What is the function of Fixative in the process?
Remove red blood cell
Why is 1-Phytohemagglutinin used?
To stimulate T-lymphocytes to division and growth
What is the function of 1-Phytohemagglutinin in the Karyotyping process?
Stimulate T-lymphocytes to division and growth
What is the role of Colcemide in the Karyotyping process?
Prevent formation of spindle fibers and breakdown existing fibers
Why is Hypotonic Solution used in the Karyotyping process?
To bulge cells
What is the function of Fixative in the Karyotyping process?
Remove red blood cells
What should be the blood collection process for chromosomal analysis?
Venous blood in green-covered lithium heparin and sodium heparin tube
Why is Trypsin used before staining in the Karyotyping process?
For chromosome digestion protein
What is the purpose of fixing the cell suspension with methanol and glacial acetic acid?
To preserve the cell structure and prevent degradation.
How is the cell suspension prepared on a slide for staining?
By dropping 3 to 4 drops of the suspension from a pasture pipette onto a wet, chilled, and grease-free slide.
What staining solution is used after preparing the slide with the cell suspension?
Giemsa stain and Sorenson's buffer in a 1:4 ratio.
How long are the glass slides left to dry after staining before trypsin treatment?
24 - 48 hours at room temperature.
What is the significance of adding trypsin solution in the karyotyping process?
To facilitate banding patterns for better chromosome visualization.
What does the presence of symbols like 't', 'B', 'R', 'fra', 'Del', or 'DC' indicate in a karyotype?
They represent different chromosomal structural abnormalities.
Test your knowledge on the functions of various reagents like Phytohemagglutinin, Colcemide, Hypotonic Solution, Fixative, and Trypsin. Additionally, learn about the steps involved in the Karyotyping method such as blood sampling and chromosome digestion.
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