Protozoa Overview and Characteristics
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Questions and Answers

Which of the following is NOT a bacterial pathogen included in the differential diagnosis of histolytica intestinal amebiasis?

  • Vibrio cholerae (correct)
  • Escherichia coli
  • Salmonella
  • Shigella
  • What is the typical dosage of metronidazole for adults with intestinal amoebiasis?

  • 500 to 750 mg orally 3 times a day for 7 to 10 days (correct)
  • 2000 mg orally 3 times a day for 7 to 10 days
  • 125 mg orally 3 times a day for 7 to 10 days
  • 1000 mg orally 3 times a day for 7 to 10 days
  • Which of the following is a characteristic of Entamoeba coli cysts?

  • They have 8 nuclei and splinter-like chromatoid bodies. (correct)
  • They contain a single chromatoid body.
  • They have a smooth, rounded shape.
  • They have a single nucleus.
  • What is NOT a preventive measure for Entamoeba histolytica infection?

    <p>Using a licensed vaccine (D)</p> Signup and view all the answers

    What is the name of the medication commonly used as first-line treatment for Entamoeba histolytica infections?

    <p>Metronidazole (D)</p> Signup and view all the answers

    Which of these is NOT listed as a possible differential diagnosis for extra-intestinal amebiasis caused by E. histolytica?

    <p>Appendicitis (D)</p> Signup and view all the answers

    What is the name of the stage of Entamoeba coli that has a sluggish movement and short, extended pseudopodia?

    <p>Trophozoite (C)</p> Signup and view all the answers

    Which of the following is NOT a characteristic of Entamoeba coli trophozoites?

    <p>They have a distinct ectoplasm and endoplasm. (A)</p> Signup and view all the answers

    What characterizes the Trophozoite stage of Entamoeba coli in comparison to Entamoeba histolytica?

    <p>It does not attack the mucosa and is considered non-pathogenic. (D)</p> Signup and view all the answers

    How can one differentiate the cyst of Entamoeba coli from that of Entamoeba histolytica?

    <p>E.coli cysts have a larger size and can have 4-8 nuclei. (D)</p> Signup and view all the answers

    What is a characteristic of Entamoeba gingivalis?

    <p>It exclusively lives in the mouth and does not invade tissues. (D)</p> Signup and view all the answers

    What kind of contact is involved in the transmission of Entamoeba gingivalis?

    <p>Oral to oral contact. (C)</p> Signup and view all the answers

    What type of vacuoles are commonly found in the Trophozoite of Entamoeba gingivalis?

    <p>Endocytotic vacuoles containing waste. (A)</p> Signup and view all the answers

    What is the term used for the cytoplasmic extensions that allow Amoeba to change shape and move?

    <p>Pseudopodia (A)</p> Signup and view all the answers

    Which stage do Amoeba primarily reproduce by binary fission?

    <p>Trophozoite stage (C)</p> Signup and view all the answers

    What is a common characteristic of protozoa?

    <p>Unicellular eukaryotic with no cell walls (B)</p> Signup and view all the answers

    What reproductive process occurs in some protozoal species like Sporozoa?

    <p>Meiotic reproduction (A)</p> Signup and view all the answers

    During which environmental conditions do Amoeba typically form an encysted stage?

    <p>During harsh or unfavorable conditions (C)</p> Signup and view all the answers

    Which part of the Amoeba's cytoplasm is primarily responsible for its movement?

    <p>Ectoplasm (C)</p> Signup and view all the answers

    What does the term 'Amoeba' derive from?

    <p>Greek for 'change' (C)</p> Signup and view all the answers

    What is the main method through which Amoeba engulf food?

    <p>Phagocytosis (A)</p> Signup and view all the answers

    What is the diagnostic stage of protozoa called?

    <p>Trophozoite (D)</p> Signup and view all the answers

    How do protozoa in the Class Rhizopoda primarily move?

    <p>Pseudopodia (A)</p> Signup and view all the answers

    Which of the following protozoa is known to be pathogenic?

    <p>Entamoeba histolytica (B)</p> Signup and view all the answers

    What are cysts in protozoa primarily characterized by?

    <p>Dormancy and resistance to environmental stress (B)</p> Signup and view all the answers

    Which of the following is NOT classified as an intestinal amoeba?

    <p>Acanthamoeba species (A)</p> Signup and view all the answers

    What type of environment is Entamoeba histolytica more commonly found in?

    <p>Tropical and subtropical countries (C)</p> Signup and view all the answers

    What is the primary method of multiplication for Trophozoites in protozoa?

    <p>Binary fission (C)</p> Signup and view all the answers

    Which free-living amoeba is associated with causing meningoencephalitis?

    <p>Naegleria fowleri (C)</p> Signup and view all the answers

    What form of E. histolytica is primarily responsible for invasiveness in the host?

    <p>Trophozoites (D)</p> Signup and view all the answers

    Which route is the most common for the transmission of amoebic cysts?

    <p>Fecal-oral route (A)</p> Signup and view all the answers

    What is a characteristic symptom of amoebic dysentery?

    <p>Bloody diarrhea with mucus and pus cells (C)</p> Signup and view all the answers

    What happens to amoebic cysts in the stomach after ingestion?

    <p>They are disintegrated by gastric juice. (D)</p> Signup and view all the answers

    During the life cycle of E. histolytica, where do trophozoites invade to cause disease?

    <p>Large intestine (D)</p> Signup and view all the answers

    Which of the following statements is NOT true regarding the cysts of E. histolytica?

    <p>They can serve as an infective form. (B)</p> Signup and view all the answers

    What are the clinical features of Intestinal Amoebiasis during the incubation period?

    <p>Symptoms can appear after one to four weeks. (D)</p> Signup and view all the answers

    What term describes the lesions caused by trophozoites invading the colonic mucosa?

    <p>Amoebic ulcers (C)</p> Signup and view all the answers

    What presents as intense colicky pain, rectal tenesmus, and more than 20 motions per day?

    <p>Fulminant colitis (B)</p> Signup and view all the answers

    In which type of stool would trophozoites typically be found?

    <p>Liquid stool (B)</p> Signup and view all the answers

    Which clinical feature is NOT associated with amoebic abscesses?

    <p>Palpable abdominal mass (A)</p> Signup and view all the answers

    What is the primary method for diagnosing intestinal amoebiasis?

    <p>Direct microscopic examination of stool (D)</p> Signup and view all the answers

    Which investigation method is specifically NOT useful for demonstrating trophozoites?

    <p>Concentration procedures (A)</p> Signup and view all the answers

    Which of the following is a characteristic of amoebic appendicitis?

    <p>Acute right lower abdominal pain (D)</p> Signup and view all the answers

    Which serological test can be used for diagnosing amoebic infections?

    <p>Indirect hemagglutination (IHA) (B)</p> Signup and view all the answers

    What is a common symptom of amoebic abscesses in extra-intestinal locations?

    <p>Weight loss (C)</p> Signup and view all the answers

    Signup and view all the answers

    Flashcards

    Protozoa

    Unicellular eukaryotic microorganisms with no cell walls.

    Amoeba

    A single-celled organism that can change its shape.

    Pseudopodia

    Cytoplasmic extensions used for movement and feeding in amoebas.

    Binary Fission

    Asexual reproduction where a single cell splits into two identical cells.

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    Trophozoite Stage

    Active, growing stage of amoebas that reproduces by binary fission.

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    Encysted Stage

    A resistant form of amoeba that survives unfavorable conditions.

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    Ectoplasm

    Outer layer of cytoplasm in amoebas, involved in shape change.

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    Endoplasm

    Inner layer of cytoplasm in amoebas, contains organelles.

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    Entamoeba histolytica

    A protozoan that causes intestinal amebiasis and extra-intestinal infections.

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    Bacterial pathogens

    Bacteria like Shigella, E.coli, Salmonella, causing intestinal diseases.

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    Preventive measures for E.histolytica

    Avoiding contaminated food/water and treating asymptomatic carriers.

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    First-line treatment for amebiasis

    Metronidazole is the primary medication for intestinal amebiasis.

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    Entamoeba coli

    A common non-pathogenic amoeba found in humans.

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    Entamoeba coli life cycle stages

    Includes trophozoite and cyst stages.

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    Symptoms of intestinal amebiasis

    Symptoms include diarrhea, abdominal pain, and weight loss.

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    Cyst structure in Entamoeba coli

    Mature cysts have 8 nuclei and splinter-like bodies.

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    Trophozoites

    The active, feeding stage of E. histolytica after ingestion.

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    Amoebic cysts

    Infective stage of E. histolytica, resistant in environment.

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    Mode of Infection

    Ways E. histolytica enters the body, mainly through food or water.

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    Life cycle of E. histolytica

    Process including ingestion of cysts and transformation into trophozoites.

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    Intestinal Amoebiasis

    Infection characterized by ulcerative lesions and dysentery symptoms.

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    Amoebic dysentery

    Severe form of intestinal amoebiasis with bloody diarrhea.

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    Virulence factors

    Properties of trophozoites that enable them to invade and cause damage.

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    Extraintestinal Amoebiasis

    Infection that spreads beyond the intestine, e.g., liver abscess.

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    Amoebic appendicitis

    A condition presenting with acute right lower abdominal pain due to amoebic infection.

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    Fulminant colitis

    A severe intestinal condition with intense pain, rectal urgency, and multiple daily motions.

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    Amoebic abscess

    An infection leading to fever, weight loss, and potential abscesses in the liver or other organs.

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    Stool examination

    Analysis of stool to find cysts or trophozoites in diagnosing intestinal amoebiasis.

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    Stool antigen detection

    Tests like ELISA to detect amoebic antigens in stool samples.

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    Serology

    Blood tests to identify amoebic antigens and antibodies for diagnosis.

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    Molecular diagnosis

    Advanced techniques like PCR used to detect Entamoeba histolytica genetic material.

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    E. gingivalis

    A parasite residing in human mouths; observed only in the trophozoite stage without cysts.

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    Ingested particles in E. coli

    E. coli trophozoite may contain RBCs and glycogen but these are not clearly visible.

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    Transmission of E. gingivalis

    Transmission occurs solely through oral contact; no cysts are formed.

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    Cyst

    The dormant, resistant stage of protozoa, often infectious.

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    Pathogenic Amoebae

    Amoebae that can cause disease, like E. histolytica.

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    Nonpathogenic Amoebae

    Amoebae that do not cause disease, like E. dispar.

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    Class Rhizopoda

    Protozoa that move by pseudopodia and reproduce via binary fission.

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    Free-living Amoebae

    Amoebae that exist outside hosts and may be opportunistic pathogens.

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    Study Notes

    Protozoa Overview

    • Protozoa are single-celled eukaryotic microorganisms.
    • They lack cell walls.
    • Many protozoa live singly, some in colonies.
    • Only a few are pathogenic to humans.

    Protozoa Reproduction

    • Asexual reproduction: Mitotic binary fission produces two new daughter cells from one parent cell.
    • Sexual reproduction: Meiotic process, occurs in some species like Sporozoa.

    Amoeba Characteristics

    • Amoeba is a single-celled protozoan that changes shape continuously.
    • Pseudopodia are cytoplasmic extensions that allow movement and engulfing of food (phagocytosis).
    • Amoeba has an outer ectoplasm layer and an inner endoplasm layer.
    • They multiply by binary fission when conditions are favorable.
    • Many species form cysts for survival during unfavorable conditions, transfer to new hosts

    Classification of Clinically Important Protozoa

    • Classified based on mode of locomotion and site of infection.
    • Amoebas move using extensions.
    • Flagellates use whip-like structures.
    • Ciliates use cilia beating.
    • Sporozoans are non-motile in adult form.
    • Intestinal: Entamoeba histolytica, Giardia lamblia, Cryptosporidium parvum, Trichomonas vaginalis
    • Blood & tissues: Plasmodium, Toxoplasma gondii, Trypanosoma, Leishmania

    Entamoeba histolytica

    • Worldwide, more common in warm regions.
    • Three subspecies: E. dispar, E. moshkovskii, E. bangladeshi.
    • Causes amoebic dysentery (intestinal) and amoebic liver abscess.
    • Has a trophozoite and cyst stage.
    • Trophozoite multiplies in the intestines, eventually cysts are formed to survive unfavorable conditions.
    • Humans get infected through ingesting cysts in contaminated food/water.
    • Diagnosis: microscopic, stool culture, antigen tests, serology, and molecular.

    Entamoeba coli

    • Cosmopolitan (found worldwide).
    • Two stages: Trophozoite and cyst.
    • Trophozoite doesn’t invade the intestinal mucosa, non-pathogenic.
    • Has 8 nuclei in the cyst stage..

    Entamoeba gingivalis

    • Primarily found in the oral cavity of humans and other mammals.
    • Only the trophozoite stage is known.
    • Non-pathogenic but can ingest bacteria, leukocytes, and erythrocytes.
    • Transmission by oral-oral contact.

    Lifecycle of E. histolytica

    • Infectious form: mature quadrinucleated cyst.
    • Cyst passes through the stomach, digestive enzymes break the cyst wall, and it transforms to the trophozoite.
    • Trophozoite multiplies in intestines, causing tissue damage and diarrhea (dysentery).
    • Cysts are formed and passed out in feces, completing the cycle

    Prevention & Treatment of E. histolytica

    • Avoidance of ingesting contaminated food and water.
    • Treatment with metronidazole or similar drugs.

    Pathogenesis of Amebiasis

    • Intestinal amoebiasis: Trophozoites invade colonic mucosa, causing ulcerative lesions and bloody diarrhea.
    • Extraintestinal amoebiasis: (e.g. liver abscess) involves the spread via blood to other organs.

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    Description

    Explore the fascinating world of protozoa in this quiz covering their characteristics, reproduction methods, and classification. Learn about the different ways these microorganisms move and the few pathogenic species that affect humans. Test your knowledge on amoebas and their unique features.

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