Protein Synthesis and Ribosomes Quiz
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Questions and Answers

The enzyme adding amino acids to form proteins in a ribosome is a

  • enolase
  • synthase
  • synthetase
  • transferase (correct)

To bind to RER, the ribosome requires

  • tRNA
  • RBS
  • GTP
  • SRP (correct)

The enzyme adding amino acids to tRNA is a

  • synthase
  • synthetase
  • transferase (correct)
  • enolase

The RNA carrying the codon is the

<p>mRNA (D)</p> Signup and view all the answers

The decoding of mRNA is performed by the

<p>small subunit (B)</p> Signup and view all the answers

N-formyl Met is part of the

<p>initiator tRNA of prokaryotes (C)</p> Signup and view all the answers

A charged tRNA means it has

<p>an amino acid attached to 3' (B)</p> Signup and view all the answers

Addition of amino acids to form the polypeptide

<p>P site (B)</p> Signup and view all the answers

The stop codon in translation

<p>does not bind to tRNA (C)</p> Signup and view all the answers

A heritable change in genetic material is called

<p>mutation (B)</p> Signup and view all the answers

A mutation from a purine to a pyrimidine is called

<p>transversion (C)</p> Signup and view all the answers

A mutation from a purine to another purine is called

<p>transition (A)</p> Signup and view all the answers

Missense is

<p>change in amino acid inserted (C)</p> Signup and view all the answers

A mutation in which one base is deleted is called

<p>frameshift (D)</p> Signup and view all the answers

Sickle cell anemia is an example of a point mutation that is

<p>missense (A)</p> Signup and view all the answers

In sickle cell anemia Glu is changed to

<p>Val (D)</p> Signup and view all the answers

When a mutation involves more than 50 base pairs (bp) is called

<p>structural variation (B)</p> Signup and view all the answers

A copy number variation refers to differences in I number of copies of a

<p>gene (C)</p> Signup and view all the answers

The constant internal environment is also known as

<p>homeostasis (D)</p> Signup and view all the answers

Regulatory proteins bind to DNA modulating the binding of

<p>RNA polymerase (C), RNA polymerase (D)</p> Signup and view all the answers

The binding motifs that regulate gene expression are present in the

<p>DNA major groove (B)</p> Signup and view all the answers

A metal that is part of a DNA-binding motif is

<p>Zn (B)</p> Signup and view all the answers

Genes involved in the same metabolic pathway are called

<p>operons (D)</p> Signup and view all the answers

The lac operon does not include an enzyme type

<p>transaminase (B)</p> Signup and view all the answers

In the presence of allolactose

<p>lactose can be made made (B)</p> Signup and view all the answers

The Irp repressor has a motif that is

<p>helix-turn-helix (D)</p> Signup and view all the answers

The role of promoters in eukaryotes'

<p>form binding sites (D)</p> Signup and view all the answers

Enhancers are

<p>binding sites (B)</p> Signup and view all the answers

The only ones that are not physically independent substances are the

<p>mediators (D)</p> Signup and view all the answers

High levels of DNA methylation indicate an

<p>inactive gene (C)</p> Signup and view all the answers

Basis of epigenetics are alterations in

<p>chromatin (D)</p> Signup and view all the answers

Posttranscription is not done with

<p>siRNA (A)</p> Signup and view all the answers

One common e of miRNA and siRNA is that both

<p>are cleaved by Dicer (B)</p> Signup and view all the answers

Proteins are degraded specifically in the

<p>proteasome (A)</p> Signup and view all the answers

The enzyme used to introduce ubiquitin into proteins is a

<p>ligase (D)</p> Signup and view all the answers

CRISP/Cas9 is used to

<p>edit DNA (D)</p> Signup and view all the answers

To make DNA of exons only, one has to use

<p>RT-PCR (B)</p> Signup and view all the answers

PCR was created in

<p>1983 (D)</p> Signup and view all the answers

The first recombinant DNA was made in

<p>1970 (D)</p> Signup and view all the answers

Reverse transcriptase makes

<p>cDNA from mRNA (C)</p> Signup and view all the answers

Flashcards

Enzyme adding amino acids to proteins

Synthetase is the enzyme responsible for adding amino acids to form proteins in ribosomes.

Ribosome binding to RER

Ribosomes bind to the rough endoplasmic reticulum (RER) with the help of signal recognition particle (SRP).

Enzyme adding amino acids to tRNA

Aminoacyl-tRNA synthetase is the enzyme that attaches amino acids to tRNA molecules.

RNA carrying the codon

mRNA carries the codon sequence that specifies the order of amino acids in a protein.

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mRNA decoding

The small ribosomal subunit decodes the mRNA sequence to assemble amino acids into a polypeptide chain.

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Initiator tRNA of prokaryotes

N-formylmethionine is the initiator tRNA in prokaryotic protein synthesis.

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Charged tRNA

A charged tRNA molecule has an amino acid attached to it.

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Amino acid attachment site

Amino acids are attached to the 3' end of tRNA.

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Protein synthesis site

The A (aminoacyl) site is the site for binding amino acids to the growing polypeptide chain.

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Stop codon in translation

Stop codons do not code for an amino acid and signal the end of protein synthesis.

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Heritable change in genetic material

A mutation is a heritable change in genetic material.

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Purine to pyrimidine change

A transition is a point mutation that changes a purine to a pyrimidine.

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Purine to purine change

A transversion is a point mutation where a purine is replaced by a pyrimidine or vice versa.

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Missense mutation

A missense mutation causes a change in one amino acid in the resulting protein.

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Base deletion

An indel, or frameshift mutation, involves the deletion of a base.

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Point mutation

A point mutation changes one base pair.

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Sickle cell anemia

Sickle cell anemia is a genetic disorder caused by a missense point mutation.

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Variable number of copies mutation

Copy number variation is an alteration in the number of copies of a DNA sequence.

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Constant internal environment

Homeostasis refers to the constancy of the internal environment within an organism.

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Regulatory proteins in DNA binding

Regulatory proteins bind to DNA and influence the binding of RNA polymerase.

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Metal in DNA binding motif

Zinc is a common metal found in DNA-binding motifs.

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Genes in same pathway

Operons are clusters of genes involved in the same metabolic pathway.

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Lac operon enzyme

The lac operon includes enzymes like beta-galactosidase, permease, and transacetylase related to lactose metabolism.

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Allolactose effect

In the presence of allolactose, lactose metabolism genes are expressed.

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Promoters in eukaryotes

Promoters in eukaryotes are DNA regions that signal initiation of transcription.

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Mechanism of promoters

Promoters influence gene expression by directing the binding of transcription factors.

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Study Notes

Protein synthesis and Ribosomes

  • Amino acid addition to proteins in ribosomes is done by synthetase enzymes, not synthase, transferase, or enolase.
  • Ribosomes need SRP (signal recognition particle) and GTP to bind to the rough endoplasmic reticulum (RER).
  • Transfer RNA (tRNA) is where amino acids are attached to by enzymes.

RNA Codon

  • Messenger RNA (mRNA) carries the codon.
  • Translation decoding is performed by the ribosome's small subunit.
  • The large subunit also functions in translation.

Initiation and Modification

  • N-formylmethionine (fMet) is part of the initiator tRNA in prokaryotes.
  • A charged tRNA has an amino acid attached to its 3' end.
  • A charged tRNA isn’t positively or negatively charged, it's part of the structure of the molecule.

Mutations

  • Mutations are heritable changes in genetic material.
  • A mutation where a purine is changed to a pyrimidine is called a transversion.
  • A change in one or more bases in a DNA sequence is a point mutation.
  • A mutation where one or more nucleotides are deleted is called an indel or frameshift mutation

Sickle Cell Anemia

  • Sickle cell anemia is an example of a point mutation.
  • In sickle cell anemia, glutamate is changed to valine.
  • Point mutations that result in a change to the amino acid sequence are called missense mutations.

Regulatory Proteins and DNA

  • Regulatory proteins and a piece of DNA bind to DNA in the major groove.
  • Metal ions, like zinc, are important components in some DNA-binding proteins.

Genetic Material and Transcriptions

  • DNA is transcribed into RNA before being translated in proteins.
  • miRNA and siRNA are small non-coding RNAs that regulate gene expression by causing cleavage or degradation of other RNA molecules.

Enhancers, Promoters, and Proteins

  • Promoters bind to areas of DNA to form sites, they encourage the binding of RNA polymerase.
  • Enhancers are regions of DNA that increase the transcription of a gene.
  • DNA methylation is a type of epigenetic modification that affects gene expression by altering the structure of DNA.

Genetic Analyses

  • PCR (polymerase chain reaction) is a technique used to amplify DNA segments.
  • Recombinant DNA technology involves combining DNA fragments from different sources.
  • Reverse transcriptase is an enzyme that makes cDNA (complementary DNA) from mRNA.

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Description

Test your knowledge on protein synthesis, the role of ribosomes, and the intricate processes involving RNA and mutations. This quiz covers essential concepts such as tRNA, mRNA, and translation mechanisms. Challenge yourself with questions related to amino acid addition and genetic mutations.

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