Podcast
Questions and Answers
What is the primary function of small nuclear RNAs (snRNAs)?
What is the primary function of small nuclear RNAs (snRNAs)?
What is the role of the spliceosome in RNA processing?
What is the role of the spliceosome in RNA processing?
Which molecules combine to form small nuclear ribonucleoproteins (snRNPs)?
Which molecules combine to form small nuclear ribonucleoproteins (snRNPs)?
During the splicing process, what does the specific adenine nucleotide interact with?
During the splicing process, what does the specific adenine nucleotide interact with?
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What is removed from pre-mRNA to form mature RNA?
What is removed from pre-mRNA to form mature RNA?
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What is the typical size range of introns?
What is the typical size range of introns?
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What is the consequence of the splicing process?
What is the consequence of the splicing process?
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What happens to the introns after they are spliced out?
What happens to the introns after they are spliced out?
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What is the primary difference between constitutive and inducible gene expression?
What is the primary difference between constitutive and inducible gene expression?
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Which of the following correctly describes the function of transcription factors?
Which of the following correctly describes the function of transcription factors?
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What is the role of alternative splicing in gene expression?
What is the role of alternative splicing in gene expression?
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Which type of RNA is primarily responsible for coding proteins?
Which type of RNA is primarily responsible for coding proteins?
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What sugar is found in RNA?
What sugar is found in RNA?
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How do extracellular cues affect gene expression?
How do extracellular cues affect gene expression?
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What is the significance of post-transcriptional regulation in gene expression?
What is the significance of post-transcriptional regulation in gene expression?
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What role do ribosomal RNAs (rRNAs) play?
What role do ribosomal RNAs (rRNAs) play?
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What is the function of promoters in transcription?
What is the function of promoters in transcription?
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Which of the following statements about DNA and RNA is correct?
Which of the following statements about DNA and RNA is correct?
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What is the main role of transfer RNAs (tRNAs)?
What is the main role of transfer RNAs (tRNAs)?
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Which type of RNA is involved in the splicing of pre-mRNA?
Which type of RNA is involved in the splicing of pre-mRNA?
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What does the transcriptional start site (TSS) signify?
What does the transcriptional start site (TSS) signify?
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What is the primary function of the basal promoter?
What is the primary function of the basal promoter?
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Which is a common feature of the TATA box?
Which is a common feature of the TATA box?
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During which phase does RNA polymerase II synthesize RNA?
During which phase does RNA polymerase II synthesize RNA?
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What happens during the termination phase of transcription?
What happens during the termination phase of transcription?
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What is the role of the 5' cap added to mRNA precursors?
What is the role of the 5' cap added to mRNA precursors?
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The process of adding a poly(A) tail is crucial for what purpose?
The process of adding a poly(A) tail is crucial for what purpose?
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Which statement correctly describes RNA polymerase II?
Which statement correctly describes RNA polymerase II?
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What is heterogenous nuclear RNA (hnRNA)?
What is heterogenous nuclear RNA (hnRNA)?
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Which sequence is part of the signal for polyadenylation in eukaryotic mRNA?
Which sequence is part of the signal for polyadenylation in eukaryotic mRNA?
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What feature distinguishes the CCAT box from the TATA box?
What feature distinguishes the CCAT box from the TATA box?
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What is the primary function of microRNA (miRNA)?
What is the primary function of microRNA (miRNA)?
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What are tRNAs primarily responsible for in protein synthesis?
What are tRNAs primarily responsible for in protein synthesis?
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What type of RNA is primarily involved in the splicing of pre-mRNA?
What type of RNA is primarily involved in the splicing of pre-mRNA?
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What percentage of the human genome is responsible for making proteins?
What percentage of the human genome is responsible for making proteins?
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How do microRNAs block gene expression?
How do microRNAs block gene expression?
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What term is used for non-coding RNAs that regulate gene expression?
What term is used for non-coding RNAs that regulate gene expression?
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What project changed the perception of non-coding DNA and RNA in 2012?
What project changed the perception of non-coding DNA and RNA in 2012?
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Which of the following is a function of non-coding RNAs?
Which of the following is a function of non-coding RNAs?
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What is the primary role of messenger RNA (mRNA) in gene expression?
What is the primary role of messenger RNA (mRNA) in gene expression?
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Which step is NOT involved in the process of transcription in eukaryotes?
Which step is NOT involved in the process of transcription in eukaryotes?
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How does alternative splicing contribute to gene regulation?
How does alternative splicing contribute to gene regulation?
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Which of the following describes a characteristic feature of transcription factors?
Which of the following describes a characteristic feature of transcription factors?
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What is the role of the 5' cap added to mRNA precursors?
What is the role of the 5' cap added to mRNA precursors?
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Which process refers to the addition of a poly(A) tail to mRNA?
Which process refers to the addition of a poly(A) tail to mRNA?
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What is the effect of chromatin structure on gene regulation?
What is the effect of chromatin structure on gene regulation?
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Which statement correctly describes the relationship between gene expression and protein synthesis?
Which statement correctly describes the relationship between gene expression and protein synthesis?
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What type of RNA is primarily responsible for carrying the genetic code from DNA to the ribosome for protein synthesis?
What type of RNA is primarily responsible for carrying the genetic code from DNA to the ribosome for protein synthesis?
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What characteristic distinguishes RNA from DNA?
What characteristic distinguishes RNA from DNA?
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Which component of RNA plays a critical role in ribosome structure and protein synthesis?
Which component of RNA plays a critical role in ribosome structure and protein synthesis?
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In the transcription process, what is the role of the promoter?
In the transcription process, what is the role of the promoter?
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Which type of RNA serves as an adaptor between mRNA and amino acids during protein synthesis?
Which type of RNA serves as an adaptor between mRNA and amino acids during protein synthesis?
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What is the primary function of small nuclear RNAs (snRNAs)?
What is the primary function of small nuclear RNAs (snRNAs)?
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Which of the following factors influences the initiation of transcription?
Which of the following factors influences the initiation of transcription?
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Which sequence within DNA signals the start of transcription?
Which sequence within DNA signals the start of transcription?
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What is the function of the poly(A) tail in mRNA stability?
What is the function of the poly(A) tail in mRNA stability?
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What determines the stability of mRNA longevity?
What determines the stability of mRNA longevity?
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What is the primary role of Poly(A) Polymerase in mRNA processing?
What is the primary role of Poly(A) Polymerase in mRNA processing?
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What occurs after the endonuclease cleaves the mRNA?
What occurs after the endonuclease cleaves the mRNA?
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Which type of RNA is primarily responsible for coding for proteins?
Which type of RNA is primarily responsible for coding for proteins?
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Which protein binds to the poly(A) tail to enhance mRNA stability?
Which protein binds to the poly(A) tail to enhance mRNA stability?
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What role do destabilizing sequences in the 3’ UTR play?
What role do destabilizing sequences in the 3’ UTR play?
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How does the length of the poly(A) tail change over the mRNA’s lifetime?
How does the length of the poly(A) tail change over the mRNA’s lifetime?
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What is the primary role of inducible transcription factors?
What is the primary role of inducible transcription factors?
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How do steroid hormones affect transcription factor activity?
How do steroid hormones affect transcription factor activity?
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What regulates the rate of transcription of a given gene?
What regulates the rate of transcription of a given gene?
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What is the function of chromatin modifiers in transcription?
What is the function of chromatin modifiers in transcription?
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What dictates the specificity of transcription factors to their target genes?
What dictates the specificity of transcription factors to their target genes?
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In which scenario would the beta cells of the pancreas turn on insulin gene expression?
In which scenario would the beta cells of the pancreas turn on insulin gene expression?
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What characteristic of inducible transcription factors allows for a dynamic response to stimuli?
What characteristic of inducible transcription factors allows for a dynamic response to stimuli?
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How does the presence of multiple binding sites for a transcription factor in a promoter affect gene expression?
How does the presence of multiple binding sites for a transcription factor in a promoter affect gene expression?
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What is the role of microRNA (miRNA) in gene expression?
What is the role of microRNA (miRNA) in gene expression?
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Which type of RNA is primarily involved in regulating gene expression and has a length of approximately 20-25 nucleotides?
Which type of RNA is primarily involved in regulating gene expression and has a length of approximately 20-25 nucleotides?
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What significant finding was highlighted by the ENCODE project regarding non-coding DNA?
What significant finding was highlighted by the ENCODE project regarding non-coding DNA?
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What function do small nuclear RNAs (snRNAs) serve in cellular processes?
What function do small nuclear RNAs (snRNAs) serve in cellular processes?
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Which component of non-coding RNA functions in the regulation of gene expression?
Which component of non-coding RNA functions in the regulation of gene expression?
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What is a primary outcome of the actions taken by microRNA on mRNA?
What is a primary outcome of the actions taken by microRNA on mRNA?
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What percentage of the human genome is primarily involved in the expression of proteins?
What percentage of the human genome is primarily involved in the expression of proteins?
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Which statement accurately describes the relationship between microRNA and mRNA?
Which statement accurately describes the relationship between microRNA and mRNA?
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What distinguishes constitutive genes from inducible genes in terms of their expression?
What distinguishes constitutive genes from inducible genes in terms of their expression?
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How do acquiescence to intracellular signals transform gene expression?
How do acquiescence to intracellular signals transform gene expression?
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Which of the following statements accurately reflects the role of transcription factors in gene regulation?
Which of the following statements accurately reflects the role of transcription factors in gene regulation?
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What is the main consequence of differential splicing in cells?
What is the main consequence of differential splicing in cells?
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What role does alternative splicing play in tissue specificity during development?
What role does alternative splicing play in tissue specificity during development?
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What is the primary function of the poly(A) tail in mRNA stability?
What is the primary function of the poly(A) tail in mRNA stability?
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What happens to the 3' UTR sequences in mRNA?
What happens to the 3' UTR sequences in mRNA?
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What key event triggers the addition of a poly(A) tail to mRNA?
What key event triggers the addition of a poly(A) tail to mRNA?
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Which RNA type is primarily responsible for carrying genetic information for protein synthesis?
Which RNA type is primarily responsible for carrying genetic information for protein synthesis?
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Which process ensures the regulation of mRNA stability over its lifecycle?
Which process ensures the regulation of mRNA stability over its lifecycle?
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Which of the following accurately describes the role of introns in mRNA?
Which of the following accurately describes the role of introns in mRNA?
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What is the approximate length of the poly(A) tail added to the mRNA 3' end?
What is the approximate length of the poly(A) tail added to the mRNA 3' end?
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How does the poly(A) tail influence the degradation of mRNA?
How does the poly(A) tail influence the degradation of mRNA?
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What is the primary mechanism by which microRNA (miRNA) regulates gene expression?
What is the primary mechanism by which microRNA (miRNA) regulates gene expression?
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What percentage of the human genome is known to encode for proteins?
What percentage of the human genome is known to encode for proteins?
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In what way did the ENCODE project change the understanding of non-coding DNA?
In what way did the ENCODE project change the understanding of non-coding DNA?
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Which of the following best describes the primary role of non-coding RNAs?
Which of the following best describes the primary role of non-coding RNAs?
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What is the typical length of microRNA (miRNA) strands?
What is the typical length of microRNA (miRNA) strands?
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What function do small nuclear RNAs (snRNAs) primarily serve in the cellular processes?
What function do small nuclear RNAs (snRNAs) primarily serve in the cellular processes?
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How do non-coding RNAs influence gene expression?
How do non-coding RNAs influence gene expression?
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What is the main reason why microRNAs (miRNAs) are considered non-coding?
What is the main reason why microRNAs (miRNAs) are considered non-coding?
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What is the function of inducible transcription factors?
What is the function of inducible transcription factors?
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How do steroid receptor transcription factors function in gene expression?
How do steroid receptor transcription factors function in gene expression?
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What is the primary role of transcription factors in regulating transcription rates?
What is the primary role of transcription factors in regulating transcription rates?
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What mechanism allows beta cells of the pancreas to respond differently based on fasting or fed states?
What mechanism allows beta cells of the pancreas to respond differently based on fasting or fed states?
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What is a consequence of the interaction between transcription factors and chromatin modifiers?
What is a consequence of the interaction between transcription factors and chromatin modifiers?
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Which statement correctly describes how transcription factors regulate gene expression?
Which statement correctly describes how transcription factors regulate gene expression?
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What is the primary function of mRNA in the context of transcription factors activating genes?
What is the primary function of mRNA in the context of transcription factors activating genes?
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Which of the following processes is affected by the binding of transcription factors to response elements?
Which of the following processes is affected by the binding of transcription factors to response elements?
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What is the role of small nuclear ribonucleoproteins (snRNPs) during RNA processing?
What is the role of small nuclear ribonucleoproteins (snRNPs) during RNA processing?
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Which statement accurately describes the process of splicing as related to the spliceosome?
Which statement accurately describes the process of splicing as related to the spliceosome?
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What happens to the exons during the splicing process?
What happens to the exons during the splicing process?
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In which phase of RNA synthesis are introns spliced out?
In which phase of RNA synthesis are introns spliced out?
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Which of the following best describes the composition of the spliceosome?
Which of the following best describes the composition of the spliceosome?
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What is formed when the 5' end of the intron becomes attached to a specific adenine nucleotide during splicing?
What is formed when the 5' end of the intron becomes attached to a specific adenine nucleotide during splicing?
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What is the arrangement of introns and exons in pre-mRNA as it undergoes splicing?
What is the arrangement of introns and exons in pre-mRNA as it undergoes splicing?
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What is one of the key features of small nuclear RNAs (snRNAs) in the context of splicing?
What is one of the key features of small nuclear RNAs (snRNAs) in the context of splicing?
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Study Notes
Ribosomes
- Ribosomes are particles important for the synthesis of new proteins
- Ribosomes are made up of ribosomal RNA (rRNA) and proteins
RNA and DNA
- RNA is a single-stranded nucleic acid that is involved in protein synthesis.
- DNA is a double-stranded nucleic acid that contains the genetic code.
- Both RNA and DNA contain the nitrogenous bases adenine (A), guanine (G), and cytosine (C).
- RNA has the nitrogenous base uracil (U) in place of thymine (T) found in DNA.
- RNA has a ribose sugar, while DNA has deoxyribose sugar.
Types of RNA
- Messenger RNA (mRNA): carries genetic information from DNA to ribosomes.
- Ribosomal RNA (rRNA): forms the core of the ribosome and catalyzes protein synthesis.
- Transfer RNA (tRNA): carries amino acids to ribosomes for protein synthesis.
- Small nuclear RNA (snRNA): involved in splicing pre-mRNA.
- Non-coding RNA (ncRNA): regulate gene expression and other cellular processes.
Transcription
- Transcription is the process of copying DNA into RNA.
- RNA polymerase II is the enzyme that catalyzes transcription.
- Promoter is a region of DNA upstream of the transcription start site (TSS).
- Enhancer elements are recognized by proteins that will aid transcription – transcription factors.
- The general transcription factors are required for RNA polymerase II binding.
- Transcription factors are proteins that bind to specific DNA sequences and regulate transcription.
- The transcription process involves initiation, elongation, and termination.
- During initiation, RNA polymerase II binds to DNA and unwinds a 17-18 bp segment of the promoter.
- During elongation, RNA polymerase II moves along the template synthesizing RNA until it reaches the terminator region.
- During termination, a polyadenylation signal sequence is recognized by RNA polymerase II, causing it to dissociate from the DNA.
Post-transcriptional Processing
- Post-transcriptional processing is the modification of RNA molecules after transcription.
- 5' capping is the addition of a 7-methylguanosine residue to the 5' end of mRNA.
- 3' polyadenylation is the addition of a poly(A) tail to the 3' end of mRNA.
- Splicing is the removal of introns from pre-mRNA.
- Spliceosome is a molecular machine that carries out splicing.
- Alternative splicing allows for the production of multiple proteins from a single gene.
Gene Regulation
- Gene regulation is the control of gene expression.
- Constitutive genes are expressed continuously.
- Inducible genes are expressed only when needed.
- Transcription factors can regulate gene expression by binding to DNA and activating or repressing transcription.
- MicroRNA (miRNA) is a small non-coding RNA that regulates gene expression.
- miRNA can downregulate gene expression by promoting RNA degradation or blocking translation.
- Transcription factors can determine tissue specificity by regulating the expression of genes specific to a particular cell type.
ENCODE
- The ENCODE project was completed in 2012 and identified that only 1% of the human genome makes protein.
- There are other elements required for gene expression such as introns, promoters and enhancers.
RNA Structure and Function
- RNA is similar to DNA but has key differences: a ribose sugar instead of deoxyribose, uracil instead of thymine, and a single-stranded structure.
- RNA is essential for copying genetic info from DNA and forming ribosomes for protein synthesis.
- mRNA (messenger RNA) carries the genetic code for proteins from DNA to ribosomes & is the "photocopy".
- rRNA (ribosomal RNA) forms the structure of ribosomes and creates proteins.
- tRNA (transfer RNA) transfers amino acids to ribosomes for protein synthesis.
Transcription and Gene Structure
- Transcription is the process of creating RNA from a DNA template.
- Gene expression is the process of turning a gene into a protein through transcription & translation.
- Genes are segments of DNA encoding for proteins.
- Genes have a promoter region, which is a DNA sequence that initiates transcription.
- Enhancers are DNA sequences that increase transcription rate.
- The Transcription Start Site (TSS) marks the beginning of transcription.
Post-Transcriptional RNA Processing
- mRNA processing modifies pre-mRNA into mature mRNA before translation.
- Introns are non-coding sequences removed from pre-mRNA.
- Exons are coding sequences spliced together in mature mRNA.
- Polyadenylation adds a "poly-A" tail to the 3' end of mRNA for stability, protecting it from degradation.
- Capping adds a 5' cap to the 5' end of mRNA, protecting it from degradation and aiding in ribosome binding.
- Alternative splicing allows multiple proteins to be produced from a single gene by splicing exons differently.
Regulation of Gene Expression
- Constitutive gene expression occurs continuously, regardless of external factors.
- Inducible gene expression is controlled by transcription factors and is responsive to external stimuli.
- Transcription factors are proteins that bind to specific DNA regions to regulate transcription.
- Transcription factor activity can regulate the rate of transcription.
- Non-coding DNA is DNA that does not code for proteins, but plays a crucial role in gene regulation.
- MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression by blocking translation or promoting degradation.
- miRNA binds to target mRNA sequences, preventing protein synthesis.
Other Key Points
- ENCODE project revealed the importance of non-coding DNA in regulating gene expression.
- Transcription factors can bind to specific DNA sequences, known as response elements, to regulate gene expression.
- Chromatin structure can influence gene expression by making DNA more or less accessible to transcription factors.
- mRNA stability is influenced by the 3' UTR (untranslated region), which determines the mRNA’s lifespan.
Polyadenylation
- 3' poly(A) tail is added to 1' transcript after the polyadenylation signal sequence (AAUAAA) is recognised.
- Endonuclease cleaves mRNA after polyadenylation signal is recognised.
- Poly(A) Polymerase adds ~ 40-250 adenine residues to the cleaved 3’ end.
Post-Transcriptional Regulation
- mRNA processing is essential for the mature mRNA molecule to be translated.
- mRNA processing involves:
- mRNA stability
- Differential mRNA splicing
mRNA Stability
- 3’ UTR sequence determines the stability of mRNA.
- The 5’ CAP protects against degradation by exonucleases.
- Exonucleases will degrade mRNA from the poly A tail.
- The poly A tail confers mRNA stability.
- Poly-A binding protein (PABP) binds to the poly A tail and stabilises mRNA.
- PABP binds approximately 30 residues.
- The poly A tail gradually shortens over the lifetime of mRNA.
- Once the poly A tail is less than 30bp, PABP no longer binds and the mRNA is degraded.
Splicing of mRNA precursors
- Introns are non-coding sequences that are removed from pre-mRNA.
- Exons are coding sequences that remain in mature mRNA.
- Introns are removed from mRNA during synthesis after the cap is added, but before the transcript is transported into the cytoplasm.
- Introns vary in size from 50 to 10,000 nucleotides.
The Spliceosome
- Splicing is carried out by a molecular machine called the spliceosome.
- The spliceosome is a complex structure made from RNA and protein.
- Small nuclear RNAs (snRNA) combine with proteins to form snRNPs (small nuclear ribonucleoproteins).
- snRNPs facilitate splicing by recognising and interacting with specific sequences at each end of the intron.
Differential Splicing
- Differential splicing allows for the production of multiple proteins from a single gene.
- Different cell types can express different isoforms of the same protein.
- The example of calcitonin processing illustrates this:
- Calcitonin is produced in the thyroid and acts as a hormone.
- cGRP (Calcitonin gene-related peptide) is produced in the brain and acts as a neurotransmitter.
Inducible Gene Expression
- Inducible gene expression allows cells to respond to environmental cues.
- Inducible transcription factors are only turned on when necessary.
- Inducible transcription factors determine tissue specificity.
Tissue Specificity
- Stem cells differentiate into different cell types through the expression of specific transcription factors.
Constitutive Gene Expression
- Constitutive genes are always expressed and are required for essential life processes.
- Examples include genes involved in metabolism, DNA repair, transcription and translation.
Inducible Gene Expression
- Inducible genes are only expressed when required.
- Inducible gene expression:
- Is controlled by transcription factors.
- Allows cells to respond to external cues such as hormones, cytokines, and cell-cell interactions.
Transcription Factors
- Transcription factors regulate the rate of transcription by:
- Binding to specific DNA sequences (response elements).
- Interacting with RNA polymerase II to promote transcription.
- Bringing in chromatin modifiers to aid unwinding.
- Bringing in coactivators.
Regulation of Gene Expression by MicroRNA (miRNA)
- miRNA are small, non-coding RNA molecules (20-25 nucleotides) that regulate gene expression.
- miRNA are transcribed from DNA but don't encode proteins.
- miRNA are partially complementary to mRNA.
- miRNA bind to specific mRNA sequences and downregulate gene expression.
Mechanisms of miRNA Activity
- miRNA can block gene expression in two ways:
- Promotion of RNA degradation: miRNA binds to complementary sequences in the 3’UTR of mRNA and induces degradation.
- Blocking of translation: miRNA binds to mRNA and blocks translation.
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Description
Test your knowledge on the roles and structures of RNA, DNA, and ribosomes in protein synthesis. This quiz covers the differences between RNA and DNA as well as the various types of RNA involved in genetic processes. Dive into the fascinating world of molecular biology and see how well you understand these essential biomolecules.