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Protein

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Questions and Answers

What is the standard method for measuring protein content in foods?

Kjeldahl method (correct)

Dumas method

Refractive index measurement

Direct measurement methods using UV-spectroscopy

What is the importance of protein in our diet?

It helps with cellular membrane function and enzyme activity, but not energy.

It has no importance in our diet.

It only provides energy.

It provides energy and helps with cellular membrane function and enzyme activity. (correct)

What is the Kjeldahl method used for measuring protein content in foods, and how is protein quantity calculated using this method?

The Kjeldahl method involves the digestion of food with a strong acid so that nitrogen is released, which is then quantified using a titration technique. Protein quantity is then calculated from the nitrogen concentration of the food using a conversion factor (usually 6.25 which is equivalent to 0.16 g nitrogen per gram of protein).

What is the disadvantage of the Kjeldahl method?

It does not measure true protein Signup and view all the answers

What is sarcopenia?

A disease that causes muscle strength and mass to decline rapidly. Signup and view all the answers

What are the disadvantages of using the Kjeldahl method for measuring protein content, and why is the conversion factor of 6.25 not suitable for all protein types?

The Kjeldahl method does not measure true protein, and the conversion/correction factor of 6.25 is not suitable for all protein types and should be corrected based on the amino acid composition of the protein in question. A number of studies have identified species-specific nitrogen correction/conversion factors, and the conversion factor of 6.25 may need to be adjusted depending on the protein being measured. Signup and view all the answers

What are some methods used to quantify protein content in foods?

Kjeldahl, Lowry, Bradford, and total amino acid content methods. Signup and view all the answers

What are the advantages and disadvantages of using UV spectrophotometric methods for measuring protein content in foods?

UV spectrophotometric methods, including the Biuret, Bradford and Lowry methods, are easy to use, not costly and can quantify small amounts of protein. However, they can give false positive protein readings depending on the sample preparation method used and the solubility of the test sample. Signup and view all the answers

Which method is recommended for measuring protein content in red seaweed?

A conversion factor of 4.59 using the Kjeldahl method Signup and view all the answers

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Description

"Test Your Knowledge on Protein: Importance, Sources, and Health Benefits" - Take this quiz to explore the significance of protein in our diet and its crucial role in maintaining overall health. From understanding the various sources of protein to its impact on preventing diseases like sarcopenia, this quiz covers it all. Challenge yourself and see how much you know about this essential nutrient!