18 Questions
What is the main function of restriction enzymes in DNA manipulation?
Cleaving DNA at specific sequences
Which of the following enzymes is responsible for joining DNA fragments together during gene cloning?
DNA Ligase
In DNA manipulation, what is the role of Methylases?
Methylating specific sequences to protect them from restriction enzymes
What is the primary function of exonucleases in DNA manipulation?
Removing nucleotides from the ends of DNA strands
Which enzyme category is responsible for cleaving phosphodiester bonds in DNA molecules?
Nucleases
What is the main purpose of gene cloning?
Creating genetically modified organisms
Which enzyme is used to cut DNA at specific recognition sequences?
Restriction enzyme
Which type of restriction enzyme requires both ATP and S-adenosylmethionine (SAM) for its function?
Type I
What is the cleavage pattern of a Type II restriction enzyme?
Cleaves the DNA within the recognition sequence
Which process involves joining DNA fragments from different sources to create a new DNA molecule?
Recombinant DNA technology
What is the role of DNA ligase in recombinant DNA technology?
To join DNA fragments together
Which of the following is NOT an application of restriction enzymes?
Protein Purification
What is the primary function of a DNA methylase enzyme?
To add methyl groups to specific DNA sequences
What is the purpose of using methylases in recombinant DNA technology?
To protect the host cell's DNA from restriction enzyme digestion
What is the purpose of bacterial transformation in gene cloning?
To amplify plasmid DNA containing the gene of interest
Which term refers to the process of inserting a gene of interest into a vector, such as a plasmid, to create a recombinant DNA molecule?
Gene cloning
What is the purpose of using DNA fragments in recombinant DNA technology?
To create new genetic combinations by inserting DNA fragments from different sources into a vector
What is the role of DNA ligase in gene cloning?
To join DNA fragments with compatible ends together
Test your knowledge on protein regulation processes such as degradation and post-translational control. Identify the correct terms related to protein targeting and activity modulation.
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