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Protein Regulation Quiz

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Questions and Answers

What is the main function of restriction enzymes in DNA manipulation?

Adding methyl groups to DNA

Cleaving DNA at specific sequences (correct)

Joining DNA fragments together

Increasing the absorbance ratio in plasmid isolation

Which of the following enzymes is responsible for joining DNA fragments together during gene cloning?

DNA Ligase (correct)

Exonucleases

Endonucleases

Methylases

In DNA manipulation, what is the role of Methylases?

Breaking phosphodiester bonds in DNA

Adding phosphate groups to DNA

Joining DNA fragments together

Methylating specific sequences to protect them from restriction enzymes (correct)

What is the primary function of exonucleases in DNA manipulation?

Removing nucleotides from the ends of DNA strands Signup and view all the answers

Which enzyme category is responsible for cleaving phosphodiester bonds in DNA molecules?

Nucleases Signup and view all the answers

What is the main purpose of gene cloning?

Creating genetically modified organisms Signup and view all the answers

Which enzyme is used to cut DNA at specific recognition sequences?

Restriction enzyme Signup and view all the answers

Which type of restriction enzyme requires both ATP and S-adenosylmethionine (SAM) for its function?

Type I Signup and view all the answers

What is the cleavage pattern of a Type II restriction enzyme?

Cleaves the DNA within the recognition sequence Signup and view all the answers

Which process involves joining DNA fragments from different sources to create a new DNA molecule?

Recombinant DNA technology Signup and view all the answers

What is the role of DNA ligase in recombinant DNA technology?

To join DNA fragments together Signup and view all the answers

Which of the following is NOT an application of restriction enzymes?

Protein Purification Signup and view all the answers

What is the primary function of a DNA methylase enzyme?

To add methyl groups to specific DNA sequences Signup and view all the answers

What is the purpose of using methylases in recombinant DNA technology?

To protect the host cell's DNA from restriction enzyme digestion Signup and view all the answers

What is the purpose of bacterial transformation in gene cloning?

To amplify plasmid DNA containing the gene of interest Signup and view all the answers

Which term refers to the process of inserting a gene of interest into a vector, such as a plasmid, to create a recombinant DNA molecule?

Gene cloning Signup and view all the answers

What is the purpose of using DNA fragments in recombinant DNA technology?

To create new genetic combinations by inserting DNA fragments from different sources into a vector Signup and view all the answers

What is the role of DNA ligase in gene cloning?

To join DNA fragments with compatible ends together Signup and view all the answers