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Questions and Answers
What is the main function of restriction enzymes in DNA manipulation?
What is the main function of restriction enzymes in DNA manipulation?
- Adding methyl groups to DNA
- Cleaving DNA at specific sequences (correct)
- Joining DNA fragments together
- Increasing the absorbance ratio in plasmid isolation
Which of the following enzymes is responsible for joining DNA fragments together during gene cloning?
Which of the following enzymes is responsible for joining DNA fragments together during gene cloning?
- DNA Ligase (correct)
- Exonucleases
- Endonucleases
- Methylases
In DNA manipulation, what is the role of Methylases?
In DNA manipulation, what is the role of Methylases?
- Breaking phosphodiester bonds in DNA
- Adding phosphate groups to DNA
- Joining DNA fragments together
- Methylating specific sequences to protect them from restriction enzymes (correct)
What is the primary function of exonucleases in DNA manipulation?
What is the primary function of exonucleases in DNA manipulation?
Which enzyme category is responsible for cleaving phosphodiester bonds in DNA molecules?
Which enzyme category is responsible for cleaving phosphodiester bonds in DNA molecules?
What is the main purpose of gene cloning?
What is the main purpose of gene cloning?
Which enzyme is used to cut DNA at specific recognition sequences?
Which enzyme is used to cut DNA at specific recognition sequences?
Which type of restriction enzyme requires both ATP and S-adenosylmethionine (SAM) for its function?
Which type of restriction enzyme requires both ATP and S-adenosylmethionine (SAM) for its function?
What is the cleavage pattern of a Type II restriction enzyme?
What is the cleavage pattern of a Type II restriction enzyme?
Which process involves joining DNA fragments from different sources to create a new DNA molecule?
Which process involves joining DNA fragments from different sources to create a new DNA molecule?
What is the role of DNA ligase in recombinant DNA technology?
What is the role of DNA ligase in recombinant DNA technology?
Which of the following is NOT an application of restriction enzymes?
Which of the following is NOT an application of restriction enzymes?
What is the primary function of a DNA methylase enzyme?
What is the primary function of a DNA methylase enzyme?
What is the purpose of using methylases in recombinant DNA technology?
What is the purpose of using methylases in recombinant DNA technology?
What is the purpose of bacterial transformation in gene cloning?
What is the purpose of bacterial transformation in gene cloning?
Which term refers to the process of inserting a gene of interest into a vector, such as a plasmid, to create a recombinant DNA molecule?
Which term refers to the process of inserting a gene of interest into a vector, such as a plasmid, to create a recombinant DNA molecule?
What is the purpose of using DNA fragments in recombinant DNA technology?
What is the purpose of using DNA fragments in recombinant DNA technology?
What is the role of DNA ligase in gene cloning?
What is the role of DNA ligase in gene cloning?
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