Protein Regulation Quiz
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Questions and Answers

What is the main function of restriction enzymes in DNA manipulation?

  • Adding methyl groups to DNA
  • Cleaving DNA at specific sequences (correct)
  • Joining DNA fragments together
  • Increasing the absorbance ratio in plasmid isolation

Which of the following enzymes is responsible for joining DNA fragments together during gene cloning?

  • DNA Ligase (correct)
  • Exonucleases
  • Endonucleases
  • Methylases

In DNA manipulation, what is the role of Methylases?

  • Breaking phosphodiester bonds in DNA
  • Adding phosphate groups to DNA
  • Joining DNA fragments together
  • Methylating specific sequences to protect them from restriction enzymes (correct)

What is the primary function of exonucleases in DNA manipulation?

<p>Removing nucleotides from the ends of DNA strands (D)</p> Signup and view all the answers

Which enzyme category is responsible for cleaving phosphodiester bonds in DNA molecules?

<p>Nucleases (C)</p> Signup and view all the answers

What is the main purpose of gene cloning?

<p>Creating genetically modified organisms (B)</p> Signup and view all the answers

Which enzyme is used to cut DNA at specific recognition sequences?

<p>Restriction enzyme (A)</p> Signup and view all the answers

Which type of restriction enzyme requires both ATP and S-adenosylmethionine (SAM) for its function?

<p>Type I (D)</p> Signup and view all the answers

What is the cleavage pattern of a Type II restriction enzyme?

<p>Cleaves the DNA within the recognition sequence (A)</p> Signup and view all the answers

Which process involves joining DNA fragments from different sources to create a new DNA molecule?

<p>Recombinant DNA technology (A)</p> Signup and view all the answers

What is the role of DNA ligase in recombinant DNA technology?

<p>To join DNA fragments together (B)</p> Signup and view all the answers

Which of the following is NOT an application of restriction enzymes?

<p>Protein Purification (D)</p> Signup and view all the answers

What is the primary function of a DNA methylase enzyme?

<p>To add methyl groups to specific DNA sequences (D)</p> Signup and view all the answers

What is the purpose of using methylases in recombinant DNA technology?

<p>To protect the host cell's DNA from restriction enzyme digestion (A)</p> Signup and view all the answers

What is the purpose of bacterial transformation in gene cloning?

<p>To amplify plasmid DNA containing the gene of interest (D)</p> Signup and view all the answers

Which term refers to the process of inserting a gene of interest into a vector, such as a plasmid, to create a recombinant DNA molecule?

<p>Gene cloning (C)</p> Signup and view all the answers

What is the purpose of using DNA fragments in recombinant DNA technology?

<p>To create new genetic combinations by inserting DNA fragments from different sources into a vector (B)</p> Signup and view all the answers

What is the role of DNA ligase in gene cloning?

<p>To join DNA fragments with compatible ends together (B)</p> Signup and view all the answers

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