20 Questions
What is the function of Pepsin?
Digest proteins into amino acids
At what pH do proteins have no net charge?
Neutral pH
How can molecular size be determined?
By ultracentrifugation
Which protein is the second most predominant in cerebrospinal fluid?
Albumin
What technique is used to differentiate proteins based on electrical charge?
Ion-exchange chromatography
Which protein is primarily produced in the liver and is considered an acute phase protein?
Haptoglobin
In which conditions would you expect to find decreased levels of haptoglobin?
Liver disease
Which protein accounts for the majority of α-2-globulin and is found principally in the intravascular spaces?
Haptoglobin
What protein is a light chain component of the major human leukocyte antigen (HLA) and found on the surface of most nucleated cells, with high concentrations on lymphocytes?
Beta2-Microglobulin
Which marker can be used for acute myocardial infarction but is not specific for this condition?
Myoglobin
In which muscle-related disorders can myoglobin levels be elevated besides acute myocardial infarction?
Cardiac and skeletal muscle disorders
Which specimen is most commonly preferred for protein testing?
Serum
In which body fluid can protein levels help differentiate between transudate and exudate?
CSF
Which factor causes false decrease in protein levels in the biuret method?
High ammonia level
Which method is preferred for rapidly estimating protein at high concentrations?
Refractometry
What causes gamma globulins to migrate towards the cathodes during electrophoresis despite having a slightly negative charge?
Electroendosmosis
Which condition is associated with an increase in the alpha-2 region and a decrease in albumin?
Nephrotic syndrome
What is the main purpose of the Folin-Ciocalteu Method?
To detect proteins in low concentrations
What is the key factor that causes false increased reference range readings in the biuret method?
High bilirubin
What is used to complex cupric ions and prevent precipitation in the Biuret method?
Rochelle salt
Test your knowledge of proteins produced in the liver and components of the major histocompatibility complex. This quiz covers characteristics and functions of acute phase proteins and HLA components.
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