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Questions and Answers
What is the process of making cells acquire and express a new piece of foreign genetic material called?
What is the process of making cells acquire and express a new piece of foreign genetic material called?
- Transfection
- Transduction
- Transformation (correct)
- Transgenic
Which term refers to the organism that has had its genome artificially altered by introducing foreign DNA sequences?
Which term refers to the organism that has had its genome artificially altered by introducing foreign DNA sequences?
- Transgene (correct)
- Transduction
- Vector/Construct
- Recombinant protein
What is the term for a DNA molecule used to carry a foreign DNA segment into a host cell?
What is the term for a DNA molecule used to carry a foreign DNA segment into a host cell?
- Transformation
- Recombinant plasmid (correct)
- Transgenic
- pGLO
Which process involves introducing recombinant plasmid to animal eukaryotic cells via artificial means?
Which process involves introducing recombinant plasmid to animal eukaryotic cells via artificial means?
What is the term used for a cell that has taken up the recombinant plasmid and acquired a new trait?
What is the term used for a cell that has taken up the recombinant plasmid and acquired a new trait?
What is the term for the protein produced by the inserted foreign DNA?
What is the term for the protein produced by the inserted foreign DNA?
What is the function of the origin of replication in plasmids?
What is the function of the origin of replication in plasmids?
What is the purpose of antibiotic resistance in genetically engineered bacteria?
What is the purpose of antibiotic resistance in genetically engineered bacteria?
Where does the GFP chromophore reside within the GFP protein structure?
Where does the GFP chromophore reside within the GFP protein structure?
What is the primary function of plasmids in genetic engineering?
What is the primary function of plasmids in genetic engineering?
How is the GFP chromophore described in terms of its structure?
How is the GFP chromophore described in terms of its structure?
What makes the GFP molecule notable in scientific circles?
What makes the GFP molecule notable in scientific circles?
What enables cells with the plasmid to grow in culture media with ampicillin?
What enables cells with the plasmid to grow in culture media with ampicillin?
What prevents the GFP gene from being transcribed in the absence of arabinose?
What prevents the GFP gene from being transcribed in the absence of arabinose?
What is the role of arabinose in the system described?
What is the role of arabinose in the system described?
Why do cells without the plasmid fail to grow on plates with ampicillin?
Why do cells without the plasmid fail to grow on plates with ampicillin?
What happens if cells with the plasmid are grown in culture media without arabinose?
What happens if cells with the plasmid are grown in culture media without arabinose?
Which protein plays a role in switching on the transcription of the GFP gene?
Which protein plays a role in switching on the transcription of the GFP gene?
What is the first step in producing a protein of interest?
What is the first step in producing a protein of interest?
What happens after the gene for a protein is identified?
What happens after the gene for a protein is identified?
What is the role of bacteria in producing a protein of interest?
What is the role of bacteria in producing a protein of interest?
Which step involves making a plasmid with the gene for production?
Which step involves making a plasmid with the gene for production?
What happens after growing lots of bacteria in the protein production process?
What happens after growing lots of bacteria in the protein production process?
In genetic engineering using plasmids, what does bacterial transformation involve?
In genetic engineering using plasmids, what does bacterial transformation involve?
What is the purpose of Hydrophobic Interaction Chromatography (HIC) in GFP purification?
What is the purpose of Hydrophobic Interaction Chromatography (HIC) in GFP purification?
What is the purpose of washing less hydrophobic proteins from the HIC column?
What is the purpose of washing less hydrophobic proteins from the HIC column?
What is the function of the Elution buffer in HIC chromatography?
What is the function of the Elution buffer in HIC chromatography?
Why is it important to discard the liquid broth supernatant before resuspending the pellet with TE buffer?
Why is it important to discard the liquid broth supernatant before resuspending the pellet with TE buffer?
Which step ensures that only the hydrophobic protein of interest remains bound to the HIC column?
Which step ensures that only the hydrophobic protein of interest remains bound to the HIC column?
What is the primary characteristic that enables GFP to be isolated effectively using Hydrophobic Interaction Chromatography?
What is the primary characteristic that enables GFP to be isolated effectively using Hydrophobic Interaction Chromatography?
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